Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France
The presence of Lactococcus bacteriophages in milk can partly or completely inhibit milk fermentation. To prevent the problems associated with the bacteriophages, the real-time PCR was developed in this study for direct detection from whey and milk of three main groups of Lactococcus bacteriophages,...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2011/594369 |
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| author | Mai Huong Ly-Chatain Loïc Durand Véronique Rigobello Annabelle Vera Yann Demarigny |
| author_facet | Mai Huong Ly-Chatain Loïc Durand Véronique Rigobello Annabelle Vera Yann Demarigny |
| author_sort | Mai Huong Ly-Chatain |
| collection | DOAJ |
| description | The presence of Lactococcus bacteriophages in milk can partly or completely inhibit milk fermentation. To prevent the problems associated with the bacteriophages, the real-time PCR was developed in this study for direct detection from whey and milk of three main groups of Lactococcus bacteriophages, c2, 936, and P335. The optimization of DNA extraction protocol from complex matrices such as whey and milk was optimized allowed the amplification of PCR without any matrix and nontarget contaminant interference. The real-time PCR program was specific and with the detection limit of 102 PFU/mL. The curve slopes were −3.49, −3.69, and −3.45 with the amplification efficiency estimated at 94%, 94%, and 98% and the correlation coefficient (𝑅2) of 0.999, 0.999, and 0.998 for c2, 936 and P335 group, respectively. This method was then used to detect the bacteriophages in whey and goat's raw milk coming from three farms located in the Rhône-Alpes region (France). |
| format | Article |
| id | doaj-art-195deaa35cf24ebea6a5ad7f3c42f478 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-195deaa35cf24ebea6a5ad7f3c42f4782025-02-03T05:45:32ZengWileyInternational Journal of Microbiology1687-918X1687-91982011-01-01201110.1155/2011/594369594369Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in FranceMai Huong Ly-Chatain0Loïc Durand1Véronique Rigobello2Annabelle Vera3Yann Demarigny4Department of Food Industry and Quality, ISARA-Lyon, 23 Jean Baldassini, 69364 Lyon Cedex 07, FranceDepartment of Food Industry and Quality, ISARA-Lyon, 23 Jean Baldassini, 69364 Lyon Cedex 07, FranceDepartment of Food Industry and Quality, ISARA-Lyon, 23 Jean Baldassini, 69364 Lyon Cedex 07, FranceDepartment of Food Industry and Quality, ISARA-Lyon, 23 Jean Baldassini, 69364 Lyon Cedex 07, FranceDepartment of Food Industry and Quality, ISARA-Lyon, 23 Jean Baldassini, 69364 Lyon Cedex 07, FranceThe presence of Lactococcus bacteriophages in milk can partly or completely inhibit milk fermentation. To prevent the problems associated with the bacteriophages, the real-time PCR was developed in this study for direct detection from whey and milk of three main groups of Lactococcus bacteriophages, c2, 936, and P335. The optimization of DNA extraction protocol from complex matrices such as whey and milk was optimized allowed the amplification of PCR without any matrix and nontarget contaminant interference. The real-time PCR program was specific and with the detection limit of 102 PFU/mL. The curve slopes were −3.49, −3.69, and −3.45 with the amplification efficiency estimated at 94%, 94%, and 98% and the correlation coefficient (𝑅2) of 0.999, 0.999, and 0.998 for c2, 936 and P335 group, respectively. This method was then used to detect the bacteriophages in whey and goat's raw milk coming from three farms located in the Rhône-Alpes region (France).http://dx.doi.org/10.1155/2011/594369 |
| spellingShingle | Mai Huong Ly-Chatain Loïc Durand Véronique Rigobello Annabelle Vera Yann Demarigny Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France International Journal of Microbiology |
| title | Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France |
| title_full | Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France |
| title_fullStr | Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France |
| title_full_unstemmed | Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France |
| title_short | Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France |
| title_sort | direct quantitative detection and identification of lactococcal bacteriophages from milk and whey by real time pcr application for the detection of lactococcal bacteriophages in goat s raw milk whey in france |
| url | http://dx.doi.org/10.1155/2011/594369 |
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