The Impact of Different Oxygen Delivery Methods on Corneal Epithelial Repair after Injury
The hyperbaric oxygen therapy is often used in the management of acid and base burns of the eyes. However, oxygen is rarely supplied locally through goggles or face mask in ophthalmology. Therefore, in this study, we aim to investigate how oxygen delivery affects eye recovery after injury. We used a...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2022/3260087 |
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| author | Shanshan Li Qingfen Tian Gang Ding Yuqin Sun Zhongkai Hao Xu Wang Chenming Zhang Yuan Tao |
| author_facet | Shanshan Li Qingfen Tian Gang Ding Yuqin Sun Zhongkai Hao Xu Wang Chenming Zhang Yuan Tao |
| author_sort | Shanshan Li |
| collection | DOAJ |
| description | The hyperbaric oxygen therapy is often used in the management of acid and base burns of the eyes. However, oxygen is rarely supplied locally through goggles or face mask in ophthalmology. Therefore, in this study, we aim to investigate how oxygen delivery affects eye recovery after injury. We used a rabbit model with corneal epithelial injury to examine the effects of local oxygen supply via goggles or face mask on the recovery of cornea. A total of 75 healthy New Zealand white rabbits were randomly divided into three groups, A, B, and C, with 25 rabbits in each group. Then, on each rabbit eye (150 eyes in total), a circle of corneal epithelium with 5 mm in diameter was scraped off from the center of the cornea with a corneal epithelial scraper. Group A was given oxygen goggles every day (the oxygen flow rate was 3 L/min, once a day, 2 hours each time); group B was given nasal inhalation of oxygen every day (the oxygen flow rate was 3 L/min, once a day, 2 hours each time); and group C did not receive any treatment and was healed naturally. We found that the group A, which received oxygen supply via goggles, showed the best eye recovery. Transmission electron microscopy showed that the cornea with local oxygen supply via goggles or face mask exhibited intact capillary structure and obvious desmosome/hemidesmosome connections between cells. Moreover, the protein and RNA levels of hypoxia-related genes were lower in group A and B, suggesting that the hypoxia factor is a sensitive and early regulator in the low oxygen environment. |
| format | Article |
| id | doaj-art-1954f1eba4f7473399f61fd226bc2525 |
| institution | Kabale University |
| issn | 2090-0058 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
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| series | Journal of Ophthalmology |
| spelling | doaj-art-1954f1eba4f7473399f61fd226bc25252025-02-03T05:57:30ZengWileyJournal of Ophthalmology2090-00582022-01-01202210.1155/2022/3260087The Impact of Different Oxygen Delivery Methods on Corneal Epithelial Repair after InjuryShanshan Li0Qingfen Tian1Gang Ding2Yuqin Sun3Zhongkai Hao4Xu Wang5Chenming Zhang6Yuan Tao7Jinan Second People’s Hospital (Jinan Eye Hospital)Jinan Second People’s Hospital (Jinan Eye Hospital)Jinan Second People’s Hospital (Jinan Eye Hospital)Jinan Second People’s Hospital (Jinan Eye Hospital)Jinan Second People’s Hospital (Jinan Eye Hospital)Jinan Second People’s Hospital (Jinan Eye Hospital)Jinan Second People’s Hospital (Jinan Eye Hospital)Jinan Second People’s Hospital (Jinan Eye Hospital)The hyperbaric oxygen therapy is often used in the management of acid and base burns of the eyes. However, oxygen is rarely supplied locally through goggles or face mask in ophthalmology. Therefore, in this study, we aim to investigate how oxygen delivery affects eye recovery after injury. We used a rabbit model with corneal epithelial injury to examine the effects of local oxygen supply via goggles or face mask on the recovery of cornea. A total of 75 healthy New Zealand white rabbits were randomly divided into three groups, A, B, and C, with 25 rabbits in each group. Then, on each rabbit eye (150 eyes in total), a circle of corneal epithelium with 5 mm in diameter was scraped off from the center of the cornea with a corneal epithelial scraper. Group A was given oxygen goggles every day (the oxygen flow rate was 3 L/min, once a day, 2 hours each time); group B was given nasal inhalation of oxygen every day (the oxygen flow rate was 3 L/min, once a day, 2 hours each time); and group C did not receive any treatment and was healed naturally. We found that the group A, which received oxygen supply via goggles, showed the best eye recovery. Transmission electron microscopy showed that the cornea with local oxygen supply via goggles or face mask exhibited intact capillary structure and obvious desmosome/hemidesmosome connections between cells. Moreover, the protein and RNA levels of hypoxia-related genes were lower in group A and B, suggesting that the hypoxia factor is a sensitive and early regulator in the low oxygen environment.http://dx.doi.org/10.1155/2022/3260087 |
| spellingShingle | Shanshan Li Qingfen Tian Gang Ding Yuqin Sun Zhongkai Hao Xu Wang Chenming Zhang Yuan Tao The Impact of Different Oxygen Delivery Methods on Corneal Epithelial Repair after Injury Journal of Ophthalmology |
| title | The Impact of Different Oxygen Delivery Methods on Corneal Epithelial Repair after Injury |
| title_full | The Impact of Different Oxygen Delivery Methods on Corneal Epithelial Repair after Injury |
| title_fullStr | The Impact of Different Oxygen Delivery Methods on Corneal Epithelial Repair after Injury |
| title_full_unstemmed | The Impact of Different Oxygen Delivery Methods on Corneal Epithelial Repair after Injury |
| title_short | The Impact of Different Oxygen Delivery Methods on Corneal Epithelial Repair after Injury |
| title_sort | impact of different oxygen delivery methods on corneal epithelial repair after injury |
| url | http://dx.doi.org/10.1155/2022/3260087 |
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