Encapsulated human islets in alginate fiber maintain long-term functionality
Maintenance of islet function after in vitro culture is crucial for both transplantation and research. Here we evaluated the effects of encapsulation in alginate fiber on the function of human islets which were distributed by the Alberta Islet Distribution Program. Encapsulated human islets from 15...
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| Format: | Article |
| Language: | English |
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The Japan Endocrine Society
2024-03-01
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| Series: | Endocrine Journal |
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| Online Access: | https://www.jstage.jst.go.jp/article/endocrj/71/3/71_EJ23-0474/_html/-char/en |
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| author | Junko Nishida Takahiro Tsuno Shigeharu G. Yabe Tatsuya Kin Satsuki Fukuda Fujie Takeda Jun Shirakawa Hitoshi Okochi |
| author_facet | Junko Nishida Takahiro Tsuno Shigeharu G. Yabe Tatsuya Kin Satsuki Fukuda Fujie Takeda Jun Shirakawa Hitoshi Okochi |
| author_sort | Junko Nishida |
| collection | DOAJ |
| description | Maintenance of islet function after in vitro culture is crucial for both transplantation and research. Here we evaluated the effects of encapsulation in alginate fiber on the function of human islets which were distributed by the Alberta Islet Distribution Program. Encapsulated human islets from 15 deceased donors were cultured under 5.5 or 25 mM glucose conditions in vitro. The amounts of C-peptide and glucagon secreted from encapsulated islets into the culture media were measured periodically, and immunohistochemical studies were performed. Encapsulated islets maintained C-peptide and glucagon secretion for more than 75 days in 5 cases; in two cases, their secretion was also successfully detected even on day 180. α- and β-cell composition and β-cell survival in islets were unaltered in the fiber after 75 or 180 days of culture. The encapsulated islets cultured with 5.5 mM glucose, but not those with 25 mM glucose, exhibited glucose responsiveness of C-peptide secretion until day 180. We demonstrate that alginate encapsulation enabled human islets to maintain their viability and glucose responsiveness of C-peptide secretion after long-term in vitro culture, potentially for more than for 180 days. |
| format | Article |
| id | doaj-art-17d1b3ba3e4c4e2ebeddff52c66c992b |
| institution | Kabale University |
| issn | 1348-4540 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | The Japan Endocrine Society |
| record_format | Article |
| series | Endocrine Journal |
| spelling | doaj-art-17d1b3ba3e4c4e2ebeddff52c66c992b2025-01-22T06:35:05ZengThe Japan Endocrine SocietyEndocrine Journal1348-45402024-03-0171325326410.1507/endocrj.EJ23-0474endocrjEncapsulated human islets in alginate fiber maintain long-term functionalityJunko Nishida0Takahiro Tsuno1Shigeharu G. Yabe2Tatsuya Kin3Satsuki Fukuda4Fujie Takeda5Jun Shirakawa6Hitoshi Okochi7Department of Regenerative Medicine, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, JapanLaboratory of Diabetes and Metabolic Disorders, Institute for Molecular and Cellular Regulation (IMCR), Gunma University, Maebashi 371-8512, JapanDepartment of Regenerative Medicine, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, JapanClinical Islet Laboratory, University of Alberta Hospital, Edmonton, Alberta T6G2C8, CanadaDepartment of Regenerative Medicine, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, JapanDepartment of Regenerative Medicine, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, JapanLaboratory of Diabetes and Metabolic Disorders, Institute for Molecular and Cellular Regulation (IMCR), Gunma University, Maebashi 371-8512, JapanDepartment of Regenerative Medicine, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, JapanMaintenance of islet function after in vitro culture is crucial for both transplantation and research. Here we evaluated the effects of encapsulation in alginate fiber on the function of human islets which were distributed by the Alberta Islet Distribution Program. Encapsulated human islets from 15 deceased donors were cultured under 5.5 or 25 mM glucose conditions in vitro. The amounts of C-peptide and glucagon secreted from encapsulated islets into the culture media were measured periodically, and immunohistochemical studies were performed. Encapsulated islets maintained C-peptide and glucagon secretion for more than 75 days in 5 cases; in two cases, their secretion was also successfully detected even on day 180. α- and β-cell composition and β-cell survival in islets were unaltered in the fiber after 75 or 180 days of culture. The encapsulated islets cultured with 5.5 mM glucose, but not those with 25 mM glucose, exhibited glucose responsiveness of C-peptide secretion until day 180. We demonstrate that alginate encapsulation enabled human islets to maintain their viability and glucose responsiveness of C-peptide secretion after long-term in vitro culture, potentially for more than for 180 days.https://www.jstage.jst.go.jp/article/endocrj/71/3/71_EJ23-0474/_html/-char/enalginatehuman isletlong-term culture |
| spellingShingle | Junko Nishida Takahiro Tsuno Shigeharu G. Yabe Tatsuya Kin Satsuki Fukuda Fujie Takeda Jun Shirakawa Hitoshi Okochi Encapsulated human islets in alginate fiber maintain long-term functionality Endocrine Journal alginate human islet long-term culture |
| title | Encapsulated human islets in alginate fiber maintain long-term functionality |
| title_full | Encapsulated human islets in alginate fiber maintain long-term functionality |
| title_fullStr | Encapsulated human islets in alginate fiber maintain long-term functionality |
| title_full_unstemmed | Encapsulated human islets in alginate fiber maintain long-term functionality |
| title_short | Encapsulated human islets in alginate fiber maintain long-term functionality |
| title_sort | encapsulated human islets in alginate fiber maintain long term functionality |
| topic | alginate human islet long-term culture |
| url | https://www.jstage.jst.go.jp/article/endocrj/71/3/71_EJ23-0474/_html/-char/en |
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