Reference genes for gene expression profiling in mouse models of Listeria monocytogenes infection

Reference genes for gene expression profiling in mouse models of Listeria monocytogenes infection

RT-qPCR dissects transcription-based processes but requires reference genes (RGs) for data normalization. This study prospected RGs for mouse macrophages (pMØ) and spleen infected with Listeria monocytogenes. The pMØ were infected in vitro with L. monocytogenes or vehicle for 4 h. Mice were injected...

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Bibliographic Details
Main Authors: Lethicia Souza Tavares, Roberta Lane Oliveira-Silva, Marcelo Tigre Moura, Jéssica Barboza da Silva, Ana Maria Benko-Iseppon, José Vitor Lima-Filho
Format: Article
Language:English
Published: Taylor & Francis Group 2023-12-01
Series:BioTechniques
Subjects:
cytokine
housekeeping genes
immune response
internal control genes
listeriosis
macrophages
Online Access:https://www.future-science.com/doi/10.2144/btn-2023-0063
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https://www.future-science.com/doi/10.2144/btn-2023-0063

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