First Report on the Emergence of <i>Neopestalotiopsis rosae</i> as a Severe Economic Threat to Strawberry Production in Germany
Strawberries hold significant economic importance in both German and global agriculture. However, their yield is often adversely affected by fungal diseases. This study describes <i>Neopestalotiopsis rosae</i> as a newly emerging pathogen responsible for leaf blight and fruit rot in stra...
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MDPI AG
2024-12-01
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| author | Tom E. Schierling Ralf T. Voegele Abbas El-Hasan |
| author_facet | Tom E. Schierling Ralf T. Voegele Abbas El-Hasan |
| author_sort | Tom E. Schierling |
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| description | Strawberries hold significant economic importance in both German and global agriculture. However, their yield is often adversely affected by fungal diseases. This study describes <i>Neopestalotiopsis rosae</i> as a newly emerging pathogen responsible for leaf blight and fruit rot in strawberries in Germany. Infected plants were observed in Hohenheim, Germany. A combination of morphological and molecular analyses, along with pathogenicity tests, confirmed the identity of <i>N. rosae</i> as the causal agent. Morphological examination of conidia and mycelium revealed key characteristics including the presence of versicolorous median cells, conidial appendages, black spherical conidiomata formation as well as changing colony color and fluffy texture. These properties align with the established descriptions for the species. Molecular analysis, particularly the sequencing of the internal transcribed spacer and β-tubulin regions allowed the precise identification of the pathogen. Artificial inoculation of healthy strawberry plants with conidial suspension derived from the isolated strain resulted in the development of characteristic symptoms, including necrotic leaf spots and water-soaked fruit lesions, similar to those observed on the original infected plants. To our knowledge, this study presents the first documented occurrence of <i>N. rosae</i> in Germany, highlighting its emergence as a significant threat to strawberry production in Europe. |
| format | Article |
| id | doaj-art-165b8a0d55b0414f8c40cb638e3512cb |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Microorganisms |
| spelling | doaj-art-165b8a0d55b0414f8c40cb638e3512cb2025-01-24T13:42:15ZengMDPI AGMicroorganisms2076-26072024-12-01131610.3390/microorganisms13010006First Report on the Emergence of <i>Neopestalotiopsis rosae</i> as a Severe Economic Threat to Strawberry Production in GermanyTom E. Schierling0Ralf T. Voegele1Abbas El-Hasan2Department of Phytopathology, Institute of Phytomedicine, Faculty of Agricultural Sciences, University of Hohenheim, Otto-Sander-Str. 5, D-70599 Stuttgart, GermanyDepartment of Phytopathology, Institute of Phytomedicine, Faculty of Agricultural Sciences, University of Hohenheim, Otto-Sander-Str. 5, D-70599 Stuttgart, GermanyDepartment of Phytopathology, Institute of Phytomedicine, Faculty of Agricultural Sciences, University of Hohenheim, Otto-Sander-Str. 5, D-70599 Stuttgart, GermanyStrawberries hold significant economic importance in both German and global agriculture. However, their yield is often adversely affected by fungal diseases. This study describes <i>Neopestalotiopsis rosae</i> as a newly emerging pathogen responsible for leaf blight and fruit rot in strawberries in Germany. Infected plants were observed in Hohenheim, Germany. A combination of morphological and molecular analyses, along with pathogenicity tests, confirmed the identity of <i>N. rosae</i> as the causal agent. Morphological examination of conidia and mycelium revealed key characteristics including the presence of versicolorous median cells, conidial appendages, black spherical conidiomata formation as well as changing colony color and fluffy texture. These properties align with the established descriptions for the species. Molecular analysis, particularly the sequencing of the internal transcribed spacer and β-tubulin regions allowed the precise identification of the pathogen. Artificial inoculation of healthy strawberry plants with conidial suspension derived from the isolated strain resulted in the development of characteristic symptoms, including necrotic leaf spots and water-soaked fruit lesions, similar to those observed on the original infected plants. To our knowledge, this study presents the first documented occurrence of <i>N. rosae</i> in Germany, highlighting its emergence as a significant threat to strawberry production in Europe.https://www.mdpi.com/2076-2607/13/1/6strawberryfirst reportfungal pathogenKoch’s postulatesPCRsequencing |
| spellingShingle | Tom E. Schierling Ralf T. Voegele Abbas El-Hasan First Report on the Emergence of <i>Neopestalotiopsis rosae</i> as a Severe Economic Threat to Strawberry Production in Germany Microorganisms strawberry first report fungal pathogen Koch’s postulates PCR sequencing |
| title | First Report on the Emergence of <i>Neopestalotiopsis rosae</i> as a Severe Economic Threat to Strawberry Production in Germany |
| title_full | First Report on the Emergence of <i>Neopestalotiopsis rosae</i> as a Severe Economic Threat to Strawberry Production in Germany |
| title_fullStr | First Report on the Emergence of <i>Neopestalotiopsis rosae</i> as a Severe Economic Threat to Strawberry Production in Germany |
| title_full_unstemmed | First Report on the Emergence of <i>Neopestalotiopsis rosae</i> as a Severe Economic Threat to Strawberry Production in Germany |
| title_short | First Report on the Emergence of <i>Neopestalotiopsis rosae</i> as a Severe Economic Threat to Strawberry Production in Germany |
| title_sort | first report on the emergence of i neopestalotiopsis rosae i as a severe economic threat to strawberry production in germany |
| topic | strawberry first report fungal pathogen Koch’s postulates PCR sequencing |
| url | https://www.mdpi.com/2076-2607/13/1/6 |
| work_keys_str_mv | AT tomeschierling firstreportontheemergenceofineopestalotiopsisrosaeiasasevereeconomicthreattostrawberryproductioningermany AT ralftvoegele firstreportontheemergenceofineopestalotiopsisrosaeiasasevereeconomicthreattostrawberryproductioningermany AT abbaselhasan firstreportontheemergenceofineopestalotiopsisrosaeiasasevereeconomicthreattostrawberryproductioningermany |