Unveiling cellular changes in leukaemia cell lines after cannabidiol treatment through lipidomics
Abstract The present study was aimed at revealing the metabolic changes that occurred in the cellular lipid pattern of acute and chronic myeloid leukaemia cells following treatment with cannabidiol (CBD). CBD is a non-psychoactive compound present in Cannabis sativa L., which has shown an antiprolif...
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Nature Portfolio
2025-01-01
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| author | David Chamoso-Sanchez Martina Panini Clarissa Caroli Matilde Marani Lorenzo Corsi Francisco J Rupérez Antonia Garcia Federica Pellati |
| author_facet | David Chamoso-Sanchez Martina Panini Clarissa Caroli Matilde Marani Lorenzo Corsi Francisco J Rupérez Antonia Garcia Federica Pellati |
| author_sort | David Chamoso-Sanchez |
| collection | DOAJ |
| description | Abstract The present study was aimed at revealing the metabolic changes that occurred in the cellular lipid pattern of acute and chronic myeloid leukaemia cells following treatment with cannabidiol (CBD). CBD is a non-psychoactive compound present in Cannabis sativa L., which has shown an antiproliferative action in these type of cancer cells. CBD treatment reduced cell viability and initiated apoptotic and necrotic processes in both cancer cell lines in a time and dose-dependent manner, showing acute myeloid leukaemia (HL-60) cells greater sensitivity than chronic myeloid leukaemia ones (K-562), without differences in the activation of caspases 3/7. Then, control and treated cells of HL-60 and K-562 cell lines were studied through an untargeted lipidomic approach. The treatment was carried out with CBD at a concentration of 10 μM for HL-60 cells and 23 µM CBD for K-562 cells for 48 h. After the extraction of the lipid content from cell lysates, the samples were analysed by UHPLC-QTOF-MS/MS both in the positive and the negative ionization modes. The comprehensive characterization of cellular lipids unveiled several classes significantly affected by CBD treatment. Most of the differences correspond to phospholipids, including cardiolipins (CL), phosphatidylcholines (PC) and phosphosphingolipids (SM), and also triacylglycerols (TG), being many TG species increased after CBD treatment in the acute and chronic models, whereas phospholipids were found to be decreased. The results highlight some important lipid alterations related to CBD treatment, plausibly connected with different metabolic mechanisms involved in the process of cell death by apoptosis in cancer cell lines. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-15e46fa1c2b54a2d8d283b56654b857b2025-01-19T12:21:18ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-025-86044-5Unveiling cellular changes in leukaemia cell lines after cannabidiol treatment through lipidomicsDavid Chamoso-Sanchez0Martina Panini1Clarissa Caroli2Matilde Marani3Lorenzo Corsi4Francisco J Rupérez5Antonia Garcia6Federica Pellati7Centro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización MontepríncipeDepartment of Life Sciences, University of Modena and Reggio EmiliaDepartment of Life Sciences, University of Modena and Reggio EmiliaDepartment of Life Sciences, University of Modena and Reggio EmiliaDepartment of Life Sciences, University of Modena and Reggio EmiliaCentro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización MontepríncipeCentro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización MontepríncipeDepartment of Life Sciences, University of Modena and Reggio EmiliaAbstract The present study was aimed at revealing the metabolic changes that occurred in the cellular lipid pattern of acute and chronic myeloid leukaemia cells following treatment with cannabidiol (CBD). CBD is a non-psychoactive compound present in Cannabis sativa L., which has shown an antiproliferative action in these type of cancer cells. CBD treatment reduced cell viability and initiated apoptotic and necrotic processes in both cancer cell lines in a time and dose-dependent manner, showing acute myeloid leukaemia (HL-60) cells greater sensitivity than chronic myeloid leukaemia ones (K-562), without differences in the activation of caspases 3/7. Then, control and treated cells of HL-60 and K-562 cell lines were studied through an untargeted lipidomic approach. The treatment was carried out with CBD at a concentration of 10 μM for HL-60 cells and 23 µM CBD for K-562 cells for 48 h. After the extraction of the lipid content from cell lysates, the samples were analysed by UHPLC-QTOF-MS/MS both in the positive and the negative ionization modes. The comprehensive characterization of cellular lipids unveiled several classes significantly affected by CBD treatment. Most of the differences correspond to phospholipids, including cardiolipins (CL), phosphatidylcholines (PC) and phosphosphingolipids (SM), and also triacylglycerols (TG), being many TG species increased after CBD treatment in the acute and chronic models, whereas phospholipids were found to be decreased. The results highlight some important lipid alterations related to CBD treatment, plausibly connected with different metabolic mechanisms involved in the process of cell death by apoptosis in cancer cell lines.https://doi.org/10.1038/s41598-025-86044-5CannabidiolLeukaemiaCancer cellsApoptosisLipidomicsUHPLC-MS/MS |
| spellingShingle | David Chamoso-Sanchez Martina Panini Clarissa Caroli Matilde Marani Lorenzo Corsi Francisco J Rupérez Antonia Garcia Federica Pellati Unveiling cellular changes in leukaemia cell lines after cannabidiol treatment through lipidomics Scientific Reports Cannabidiol Leukaemia Cancer cells Apoptosis Lipidomics UHPLC-MS/MS |
| title | Unveiling cellular changes in leukaemia cell lines after cannabidiol treatment through lipidomics |
| title_full | Unveiling cellular changes in leukaemia cell lines after cannabidiol treatment through lipidomics |
| title_fullStr | Unveiling cellular changes in leukaemia cell lines after cannabidiol treatment through lipidomics |
| title_full_unstemmed | Unveiling cellular changes in leukaemia cell lines after cannabidiol treatment through lipidomics |
| title_short | Unveiling cellular changes in leukaemia cell lines after cannabidiol treatment through lipidomics |
| title_sort | unveiling cellular changes in leukaemia cell lines after cannabidiol treatment through lipidomics |
| topic | Cannabidiol Leukaemia Cancer cells Apoptosis Lipidomics UHPLC-MS/MS |
| url | https://doi.org/10.1038/s41598-025-86044-5 |
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