Protection against SHIV-KB9 Infection by Combining rDNA and rFPV Vaccines Based on HIV Multiepitope and p24 Protein in Chinese Rhesus Macaques
Developing an effective vaccine against HIV infection remains an urgent goal. We used a DNA prime/fowlpox virus boost regimen to immunize Chinese rhesus macaques. The animals were challenged intramuscularly with pathogenic molecularly cloned SHIV-KB9. Immunogenicity and protective efficacy of vaccin...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/958404 |
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| author | Chang Li Zhenwei Shen Xiao Li Jieying Bai Lin Zeng Mingyao Tian Ying Jin Song Ming Ye Shouwen Du Dayong Ren Cunxia Liu Na Zhu Dandan Sun Yi Li Ningyi Jin |
| author_facet | Chang Li Zhenwei Shen Xiao Li Jieying Bai Lin Zeng Mingyao Tian Ying Jin Song Ming Ye Shouwen Du Dayong Ren Cunxia Liu Na Zhu Dandan Sun Yi Li Ningyi Jin |
| author_sort | Chang Li |
| collection | DOAJ |
| description | Developing an effective vaccine against HIV infection remains an urgent goal. We used a DNA prime/fowlpox virus boost regimen to immunize Chinese rhesus macaques. The animals were challenged intramuscularly with pathogenic molecularly cloned SHIV-KB9. Immunogenicity and protective efficacy of vaccines were investigated by measuring IFN-γ levels, monitoring HIV-specific binding antibodies, examining viral load, and analyzing CD4/CD8 ratio. Results show that, upon challenge, the vaccine group can induce a strong immune response in the body, represented by increased expression of IFN-γ, slow and steady elevated antibody production, reduced peak value of acute viral load, and increase in the average CD4/CD8 ratio. The current research suggests that rapid reaction speed, appropriate response strength, and long-lasting immune response time may be key protection factors for AIDS vaccine. The present study contributes significantly to AIDS vaccine and preclinical research. |
| format | Article |
| id | doaj-art-15bff8fe8dab4e82831a47003bb8d008 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-15bff8fe8dab4e82831a47003bb8d0082025-02-03T05:49:41ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/958404958404Protection against SHIV-KB9 Infection by Combining rDNA and rFPV Vaccines Based on HIV Multiepitope and p24 Protein in Chinese Rhesus MacaquesChang Li0Zhenwei Shen1Xiao Li2Jieying Bai3Lin Zeng4Mingyao Tian5Ying Jin Song6Ming Ye7Shouwen Du8Dayong Ren9Cunxia Liu10Na Zhu11Dandan Sun12Yi Li13Ningyi Jin14Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaLaboratory Animal Center, Academy of Military Medical Sciences, Dongda Street No. 20, Beijing 100071, ChinaLaboratory Animal Center, Academy of Military Medical Sciences, Dongda Street No. 20, Beijing 100071, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaSchool of Agriculture and Biological Engineering, Tianjin University, Weijin Road No. 92, Tianjin 300072, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, LiuYingXi Road No. 666, Changchun 130122, ChinaDeveloping an effective vaccine against HIV infection remains an urgent goal. We used a DNA prime/fowlpox virus boost regimen to immunize Chinese rhesus macaques. The animals were challenged intramuscularly with pathogenic molecularly cloned SHIV-KB9. Immunogenicity and protective efficacy of vaccines were investigated by measuring IFN-γ levels, monitoring HIV-specific binding antibodies, examining viral load, and analyzing CD4/CD8 ratio. Results show that, upon challenge, the vaccine group can induce a strong immune response in the body, represented by increased expression of IFN-γ, slow and steady elevated antibody production, reduced peak value of acute viral load, and increase in the average CD4/CD8 ratio. The current research suggests that rapid reaction speed, appropriate response strength, and long-lasting immune response time may be key protection factors for AIDS vaccine. The present study contributes significantly to AIDS vaccine and preclinical research.http://dx.doi.org/10.1155/2012/958404 |
| spellingShingle | Chang Li Zhenwei Shen Xiao Li Jieying Bai Lin Zeng Mingyao Tian Ying Jin Song Ming Ye Shouwen Du Dayong Ren Cunxia Liu Na Zhu Dandan Sun Yi Li Ningyi Jin Protection against SHIV-KB9 Infection by Combining rDNA and rFPV Vaccines Based on HIV Multiepitope and p24 Protein in Chinese Rhesus Macaques Clinical and Developmental Immunology |
| title | Protection against SHIV-KB9 Infection by Combining rDNA and rFPV Vaccines Based on HIV Multiepitope and p24 Protein in Chinese Rhesus Macaques |
| title_full | Protection against SHIV-KB9 Infection by Combining rDNA and rFPV Vaccines Based on HIV Multiepitope and p24 Protein in Chinese Rhesus Macaques |
| title_fullStr | Protection against SHIV-KB9 Infection by Combining rDNA and rFPV Vaccines Based on HIV Multiepitope and p24 Protein in Chinese Rhesus Macaques |
| title_full_unstemmed | Protection against SHIV-KB9 Infection by Combining rDNA and rFPV Vaccines Based on HIV Multiepitope and p24 Protein in Chinese Rhesus Macaques |
| title_short | Protection against SHIV-KB9 Infection by Combining rDNA and rFPV Vaccines Based on HIV Multiepitope and p24 Protein in Chinese Rhesus Macaques |
| title_sort | protection against shiv kb9 infection by combining rdna and rfpv vaccines based on hiv multiepitope and p24 protein in chinese rhesus macaques |
| url | http://dx.doi.org/10.1155/2012/958404 |
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