Bioadhesive Chitosan Films Loading Curcumin for Safe and Effective Skin Cancer Topical Treatment
<b>Background/Objectives</b>: This study aimed to evaluate the safety and efficacy of chitosan-based bioadhesive films for facilitating the topical delivery of curcumin in skin cancer treatment, addressing the pharmacokinetic limitations associated with oral administration. <b>Meth...
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| Format: | Article |
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MDPI AG
2024-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/17/1/18 |
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| author | Seila Tolentino Mylene M. Monteiro Felipe Saldanha-Araújo Marcilio Cunha-Filho Tais Gratieri Eliete N. Silva Guerra Guilherme M. Gelfuso |
| author_facet | Seila Tolentino Mylene M. Monteiro Felipe Saldanha-Araújo Marcilio Cunha-Filho Tais Gratieri Eliete N. Silva Guerra Guilherme M. Gelfuso |
| author_sort | Seila Tolentino |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: This study aimed to evaluate the safety and efficacy of chitosan-based bioadhesive films for facilitating the topical delivery of curcumin in skin cancer treatment, addressing the pharmacokinetic limitations associated with oral administration. <b>Methods</b>: The films, which incorporated curcumin, were formulated using varying proportions of chitosan, polyvinyl alcohol, Poloxamer<sup>®</sup> 407, and propylene glycol. These films were assessed for stability, drug release, in vitro skin permeation, cell viability (with and without radiotherapy), and skin irritation. <b>Results</b>: The films demonstrated physical stability and preserved curcumin content at room temperature for 90 days. Drug release was effectively controlled during the first 8 h, with release rates ranging from 51.6 ± 4.8% to 65.6 ± 13.0%. The films also enhanced drug penetration into the skin compared to a curcumin solution used as a control (stratum corneum: 1.3 ± 0.1 to 1.9 ± 0.8 µg/cm²; deeper skin layers: 1.7 ± 0.1 to 2.7 ± 0.2 µg/cm²). A cytotoxicity test on metastatic melanoma cells showed that curcumin at topical doses exerted activity similar to that delivered via the skin. Furthermore, curcumin alone was more effective in inhibiting tumor cells than radiotherapy alone (<i>p</i> < 0.01), with no additional benefit observed when curcumin was combined with radiotherapy. Finally, irritation tests confirmed that the films were safe for topical application. <b>Conclusion</b>: The developed chitosan-based bioadhesive films represent a promising alternative for the topical treatment of skin tumors using curcumin. |
| format | Article |
| id | doaj-art-1575ffc2f4a64af18d7d311d8176bc1b |
| institution | Kabale University |
| issn | 1999-4923 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj-art-1575ffc2f4a64af18d7d311d8176bc1b2025-01-24T13:45:35ZengMDPI AGPharmaceutics1999-49232024-12-011711810.3390/pharmaceutics17010018Bioadhesive Chitosan Films Loading Curcumin for Safe and Effective Skin Cancer Topical TreatmentSeila Tolentino0Mylene M. Monteiro1Felipe Saldanha-Araújo2Marcilio Cunha-Filho3Tais Gratieri4Eliete N. Silva Guerra5Guilherme M. Gelfuso6Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, Brasília 70910-900, BrazilLaboratory of Oral Histopathology, University of Brasilia, Brasília 70910-900, BrazilLaboratory of Hematology and Stem Cells (LHCT), School of Health Sciences, University of Brasília, Brasília 70910-900, BrazilLaboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, Brasília 70910-900, BrazilLaboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, Brasília 70910-900, BrazilLaboratory of Oral Histopathology, University of Brasilia, Brasília 70910-900, BrazilLaboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, Brasília 70910-900, Brazil<b>Background/Objectives</b>: This study aimed to evaluate the safety and efficacy of chitosan-based bioadhesive films for facilitating the topical delivery of curcumin in skin cancer treatment, addressing the pharmacokinetic limitations associated with oral administration. <b>Methods</b>: The films, which incorporated curcumin, were formulated using varying proportions of chitosan, polyvinyl alcohol, Poloxamer<sup>®</sup> 407, and propylene glycol. These films were assessed for stability, drug release, in vitro skin permeation, cell viability (with and without radiotherapy), and skin irritation. <b>Results</b>: The films demonstrated physical stability and preserved curcumin content at room temperature for 90 days. Drug release was effectively controlled during the first 8 h, with release rates ranging from 51.6 ± 4.8% to 65.6 ± 13.0%. The films also enhanced drug penetration into the skin compared to a curcumin solution used as a control (stratum corneum: 1.3 ± 0.1 to 1.9 ± 0.8 µg/cm²; deeper skin layers: 1.7 ± 0.1 to 2.7 ± 0.2 µg/cm²). A cytotoxicity test on metastatic melanoma cells showed that curcumin at topical doses exerted activity similar to that delivered via the skin. Furthermore, curcumin alone was more effective in inhibiting tumor cells than radiotherapy alone (<i>p</i> < 0.01), with no additional benefit observed when curcumin was combined with radiotherapy. Finally, irritation tests confirmed that the films were safe for topical application. <b>Conclusion</b>: The developed chitosan-based bioadhesive films represent a promising alternative for the topical treatment of skin tumors using curcumin.https://www.mdpi.com/1999-4923/17/1/18cancerdrug deliverybioadhesive filmskintopical delivery |
| spellingShingle | Seila Tolentino Mylene M. Monteiro Felipe Saldanha-Araújo Marcilio Cunha-Filho Tais Gratieri Eliete N. Silva Guerra Guilherme M. Gelfuso Bioadhesive Chitosan Films Loading Curcumin for Safe and Effective Skin Cancer Topical Treatment Pharmaceutics cancer drug delivery bioadhesive film skin topical delivery |
| title | Bioadhesive Chitosan Films Loading Curcumin for Safe and Effective Skin Cancer Topical Treatment |
| title_full | Bioadhesive Chitosan Films Loading Curcumin for Safe and Effective Skin Cancer Topical Treatment |
| title_fullStr | Bioadhesive Chitosan Films Loading Curcumin for Safe and Effective Skin Cancer Topical Treatment |
| title_full_unstemmed | Bioadhesive Chitosan Films Loading Curcumin for Safe and Effective Skin Cancer Topical Treatment |
| title_short | Bioadhesive Chitosan Films Loading Curcumin for Safe and Effective Skin Cancer Topical Treatment |
| title_sort | bioadhesive chitosan films loading curcumin for safe and effective skin cancer topical treatment |
| topic | cancer drug delivery bioadhesive film skin topical delivery |
| url | https://www.mdpi.com/1999-4923/17/1/18 |
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