Inactivation of Zika Virus with Hydroxypropyl-Beta-Cyclodextrin
<b>Background/Objectives</b>: Zika virus (ZIKV) infection is associated with life-threatening diseases in humans. To date, there are no available FDA-approved therapies or vaccines for the specific treatment or prevention of ZIKV infection. Variation in the ZIKV envelope protein (Env), a...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/13/1/79 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587337366765568 |
|---|---|
| author | Cory R. Hewitt Nicholas J. Wixon Arthur Gallegos You Zhou Victor C. Huber M. Scott Killian |
| author_facet | Cory R. Hewitt Nicholas J. Wixon Arthur Gallegos You Zhou Victor C. Huber M. Scott Killian |
| author_sort | Cory R. Hewitt |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Zika virus (ZIKV) infection is associated with life-threatening diseases in humans. To date, there are no available FDA-approved therapies or vaccines for the specific treatment or prevention of ZIKV infection. Variation in the ZIKV envelope protein (Env), along with its complex quaternary structure, presents challenges to synthetic approaches for developing an effective vaccine and broadly neutralizing antibodies (bnAbs). We hypothesized that beta-cyclodextrin (BCD) could be used to uniquely inactivate infectious ZIKV without disruption of Env. <b>Methods</b>: ZIKV was propagated in Vero cells and admixed with BCD. The BCD-treated ZIKV was evaluated for infectivity using immunofluorescence and quantitative RT-PCR (qRT-PCR) assays, for immunoreactivity in Western blots, structural integrity by electron microscopy, and immunogenicity in mice. <b>Results</b>: Here, we show that 200 mM BCD-treated ZIKV is non-infectious in cell culture, remains immunoreactive with an Env-specific antibody, retains its virion shape and size, and elicits the production of immunogen-specific antibodies in immunized mice. <b>Conclusions</b>: These results indicate that BCD can be used to safely inactivate ZIKV, and they provide insights for vaccine and antibody development. |
| format | Article |
| id | doaj-art-154e7f37156a41cf9fdf5b25940a75d4 |
| institution | Kabale University |
| issn | 2076-393X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj-art-154e7f37156a41cf9fdf5b25940a75d42025-01-24T13:51:52ZengMDPI AGVaccines2076-393X2025-01-011317910.3390/vaccines13010079Inactivation of Zika Virus with Hydroxypropyl-Beta-CyclodextrinCory R. Hewitt0Nicholas J. Wixon1Arthur Gallegos2You Zhou3Victor C. Huber4M. Scott Killian5Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, USADivision of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, USADivision of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, USAMicroscopy Core Research Facility, Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE 68588, USADivision of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, USADivision of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, USA<b>Background/Objectives</b>: Zika virus (ZIKV) infection is associated with life-threatening diseases in humans. To date, there are no available FDA-approved therapies or vaccines for the specific treatment or prevention of ZIKV infection. Variation in the ZIKV envelope protein (Env), along with its complex quaternary structure, presents challenges to synthetic approaches for developing an effective vaccine and broadly neutralizing antibodies (bnAbs). We hypothesized that beta-cyclodextrin (BCD) could be used to uniquely inactivate infectious ZIKV without disruption of Env. <b>Methods</b>: ZIKV was propagated in Vero cells and admixed with BCD. The BCD-treated ZIKV was evaluated for infectivity using immunofluorescence and quantitative RT-PCR (qRT-PCR) assays, for immunoreactivity in Western blots, structural integrity by electron microscopy, and immunogenicity in mice. <b>Results</b>: Here, we show that 200 mM BCD-treated ZIKV is non-infectious in cell culture, remains immunoreactive with an Env-specific antibody, retains its virion shape and size, and elicits the production of immunogen-specific antibodies in immunized mice. <b>Conclusions</b>: These results indicate that BCD can be used to safely inactivate ZIKV, and they provide insights for vaccine and antibody development.https://www.mdpi.com/2076-393X/13/1/79Zika virusbeta-cyclodextrinenvelope proteinsvaccineantibodyimmunogen |
| spellingShingle | Cory R. Hewitt Nicholas J. Wixon Arthur Gallegos You Zhou Victor C. Huber M. Scott Killian Inactivation of Zika Virus with Hydroxypropyl-Beta-Cyclodextrin Vaccines Zika virus beta-cyclodextrin envelope proteins vaccine antibody immunogen |
| title | Inactivation of Zika Virus with Hydroxypropyl-Beta-Cyclodextrin |
| title_full | Inactivation of Zika Virus with Hydroxypropyl-Beta-Cyclodextrin |
| title_fullStr | Inactivation of Zika Virus with Hydroxypropyl-Beta-Cyclodextrin |
| title_full_unstemmed | Inactivation of Zika Virus with Hydroxypropyl-Beta-Cyclodextrin |
| title_short | Inactivation of Zika Virus with Hydroxypropyl-Beta-Cyclodextrin |
| title_sort | inactivation of zika virus with hydroxypropyl beta cyclodextrin |
| topic | Zika virus beta-cyclodextrin envelope proteins vaccine antibody immunogen |
| url | https://www.mdpi.com/2076-393X/13/1/79 |
| work_keys_str_mv | AT coryrhewitt inactivationofzikaviruswithhydroxypropylbetacyclodextrin AT nicholasjwixon inactivationofzikaviruswithhydroxypropylbetacyclodextrin AT arthurgallegos inactivationofzikaviruswithhydroxypropylbetacyclodextrin AT youzhou inactivationofzikaviruswithhydroxypropylbetacyclodextrin AT victorchuber inactivationofzikaviruswithhydroxypropylbetacyclodextrin AT mscottkillian inactivationofzikaviruswithhydroxypropylbetacyclodextrin |