Umbilical Cord-Derived Mesenchymal Stem Cells Relieve Hindlimb Ischemia through Enhancing Angiogenesis in Tree Shrews
Hindlimb ischemia is still a clinical problem with high morbidity and mortality. Patients suffer from consequent rest pain, ulcers, cool limbs, and even amputation. Angiogenesis is a promising target for the treatment of ischemic limbs, providing extra blood for the ischemic region. In the present s...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/9742034 |
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| author | Cunping Yin Yuan Liang Jian Zhang Guangping Ruan Zian Li Rongqing Pang Xinghua Pan |
| author_facet | Cunping Yin Yuan Liang Jian Zhang Guangping Ruan Zian Li Rongqing Pang Xinghua Pan |
| author_sort | Cunping Yin |
| collection | DOAJ |
| description | Hindlimb ischemia is still a clinical problem with high morbidity and mortality. Patients suffer from consequent rest pain, ulcers, cool limbs, and even amputation. Angiogenesis is a promising target for the treatment of ischemic limbs, providing extra blood for the ischemic region. In the present study, we investigated the role of umbilical cord-derived mesenchymal stem cells (UC-MSCs) in regulating angiogenesis and relieving hindlimb ischemia. UC-MSCs were isolated from the umbilical cord of tree shrews. Angiography results showed that UC-MSCs injection significantly promoted angiogenesis in tree shrews. Moreover, the ankle brachial index, transcutaneous oxygen pressure, blood perfusion, and capillary/muscle fiber ratio were all markedly increased by the application of UC-MSCs. In addition, the conditioned culture of human umbilical vein endothelial cells using medium collected from UC-MSCs showed higher expression of angiogenic markers and improved migration ability. In short, the isolated UC-MSCs notably contributed to restoring blood supply and alleviating the symptoms of limb ischemia through enhancing angiogenesis. |
| format | Article |
| id | doaj-art-150c3550e4854cd59cf7fb2309c71eab |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-150c3550e4854cd59cf7fb2309c71eab2025-02-03T01:06:27ZengWileyStem Cells International1687-96782016-01-01201610.1155/2016/97420349742034Umbilical Cord-Derived Mesenchymal Stem Cells Relieve Hindlimb Ischemia through Enhancing Angiogenesis in Tree ShrewsCunping Yin0Yuan Liang1Jian Zhang2Guangping Ruan3Zian Li4Rongqing Pang5Xinghua Pan6Department of Vascular SurgeryDepartment of GeriatricsDepartment of Medical ImagingStem Cell Engineering Laboratory of Yunnan ProvinceStem Cell Engineering Laboratory of Yunnan ProvinceStem Cell Engineering Laboratory of Yunnan ProvinceStem Cell Engineering Laboratory of Yunnan ProvinceHindlimb ischemia is still a clinical problem with high morbidity and mortality. Patients suffer from consequent rest pain, ulcers, cool limbs, and even amputation. Angiogenesis is a promising target for the treatment of ischemic limbs, providing extra blood for the ischemic region. In the present study, we investigated the role of umbilical cord-derived mesenchymal stem cells (UC-MSCs) in regulating angiogenesis and relieving hindlimb ischemia. UC-MSCs were isolated from the umbilical cord of tree shrews. Angiography results showed that UC-MSCs injection significantly promoted angiogenesis in tree shrews. Moreover, the ankle brachial index, transcutaneous oxygen pressure, blood perfusion, and capillary/muscle fiber ratio were all markedly increased by the application of UC-MSCs. In addition, the conditioned culture of human umbilical vein endothelial cells using medium collected from UC-MSCs showed higher expression of angiogenic markers and improved migration ability. In short, the isolated UC-MSCs notably contributed to restoring blood supply and alleviating the symptoms of limb ischemia through enhancing angiogenesis.http://dx.doi.org/10.1155/2016/9742034 |
| spellingShingle | Cunping Yin Yuan Liang Jian Zhang Guangping Ruan Zian Li Rongqing Pang Xinghua Pan Umbilical Cord-Derived Mesenchymal Stem Cells Relieve Hindlimb Ischemia through Enhancing Angiogenesis in Tree Shrews Stem Cells International |
| title | Umbilical Cord-Derived Mesenchymal Stem Cells Relieve Hindlimb Ischemia through Enhancing Angiogenesis in Tree Shrews |
| title_full | Umbilical Cord-Derived Mesenchymal Stem Cells Relieve Hindlimb Ischemia through Enhancing Angiogenesis in Tree Shrews |
| title_fullStr | Umbilical Cord-Derived Mesenchymal Stem Cells Relieve Hindlimb Ischemia through Enhancing Angiogenesis in Tree Shrews |
| title_full_unstemmed | Umbilical Cord-Derived Mesenchymal Stem Cells Relieve Hindlimb Ischemia through Enhancing Angiogenesis in Tree Shrews |
| title_short | Umbilical Cord-Derived Mesenchymal Stem Cells Relieve Hindlimb Ischemia through Enhancing Angiogenesis in Tree Shrews |
| title_sort | umbilical cord derived mesenchymal stem cells relieve hindlimb ischemia through enhancing angiogenesis in tree shrews |
| url | http://dx.doi.org/10.1155/2016/9742034 |
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