Level of CYP4G19 Expression Is Associated with Pyrethroid Resistance in Blattella germanica
German cockroaches have become a large problem in the Shenzhen area because of their pesticide resistance, especially to pyrethroid. A pyrethroid called “Jia Chong Qing” to prevent pests for a long time were found to be resistant to “Jia Chong Qing” with resistance index of 3.88 measured using RT-...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2010/517534 |
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| author | Guang-zhou Guo Yi-jie Geng Da-na Huang Cai-fang Xue Ren-li Zhang |
| author_facet | Guang-zhou Guo Yi-jie Geng Da-na Huang Cai-fang Xue Ren-li Zhang |
| author_sort | Guang-zhou Guo |
| collection | DOAJ |
| description | German cockroaches have become a large problem in the Shenzhen area because of their pesticide resistance, especially to pyrethroid. A pyrethroid called “Jia Chong Qing” to prevent pests for a long time were found to be resistant to “Jia Chong Qing” with resistance index of 3.88 measured using RT-PCR and immunohistochemistry analysis showed that both CYP4G19 mRNA and CYP4G19 protein expression levels in the wild strain were substantially higher than that of a sensitive strain. dsRNA segments derived from the target gene CYP4G19 were prepared using in vitro transcription and were microinjected into abdomens of the wild strain. Two to eight days after injection, the result showed that CYP4G19 mRNA expressions were significantly reduced in the groups injected with dsRNAs. |
| format | Article |
| id | doaj-art-14c619b4132c48ee9bcf3086f803f70f |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-14c619b4132c48ee9bcf3086f803f70f2025-02-03T01:22:44ZengWileyJournal of Parasitology Research2090-00232090-00312010-01-01201010.1155/2010/517534517534Level of CYP4G19 Expression Is Associated with Pyrethroid Resistance in Blattella germanicaGuang-zhou Guo0Yi-jie Geng1Da-na Huang2Cai-fang Xue3Ren-li Zhang4Department of Pathogenic Organism, Fourth Military Medical University, Xian 710032, ChinaShenzhen Centre for Diseases Control and Prevention, Shenzhen 518020, ChinaShenzhen Centre for Diseases Control and Prevention, Shenzhen 518020, ChinaDepartment of Pathogenic Organism, Fourth Military Medical University, Xian 710032, ChinaShenzhen Centre for Diseases Control and Prevention, Shenzhen 518020, ChinaGerman cockroaches have become a large problem in the Shenzhen area because of their pesticide resistance, especially to pyrethroid. A pyrethroid called “Jia Chong Qing” to prevent pests for a long time were found to be resistant to “Jia Chong Qing” with resistance index of 3.88 measured using RT-PCR and immunohistochemistry analysis showed that both CYP4G19 mRNA and CYP4G19 protein expression levels in the wild strain were substantially higher than that of a sensitive strain. dsRNA segments derived from the target gene CYP4G19 were prepared using in vitro transcription and were microinjected into abdomens of the wild strain. Two to eight days after injection, the result showed that CYP4G19 mRNA expressions were significantly reduced in the groups injected with dsRNAs.http://dx.doi.org/10.1155/2010/517534 |
| spellingShingle | Guang-zhou Guo Yi-jie Geng Da-na Huang Cai-fang Xue Ren-li Zhang Level of CYP4G19 Expression Is Associated with Pyrethroid Resistance in Blattella germanica Journal of Parasitology Research |
| title | Level of CYP4G19 Expression Is Associated with Pyrethroid Resistance in Blattella germanica |
| title_full | Level of CYP4G19 Expression Is Associated with Pyrethroid Resistance in Blattella germanica |
| title_fullStr | Level of CYP4G19 Expression Is Associated with Pyrethroid Resistance in Blattella germanica |
| title_full_unstemmed | Level of CYP4G19 Expression Is Associated with Pyrethroid Resistance in Blattella germanica |
| title_short | Level of CYP4G19 Expression Is Associated with Pyrethroid Resistance in Blattella germanica |
| title_sort | level of cyp4g19 expression is associated with pyrethroid resistance in blattella germanica |
| url | http://dx.doi.org/10.1155/2010/517534 |
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