Exploring the Anti-Osteoporotic Effects of <i>n</i>-Hexane Fraction from <i>Cotoneaster wilsonii</i> Nakai: Activation of Runx2 and Osteoblast Differentiation In Vivo
Background: Osteoporosis is characterized by the microstructural depletion of bone tissue and decreased bone density, leading to an increased risk of fractures. <i>Cotoneaster wilsonii</i> Nakai, an endemic species of the Korean Peninsula, grows wild in Ulleungdo. In this study, we aimed...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/18/1/45 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587692136726528 |
|---|---|
| author | Soyeon Hong Hee Ju Lee Da Seul Jung Saruul Erdenebileg Hoseong Hwang Hak Cheol Kwon Jaeyoung Kwon Gyhye Yoo |
| author_facet | Soyeon Hong Hee Ju Lee Da Seul Jung Saruul Erdenebileg Hoseong Hwang Hak Cheol Kwon Jaeyoung Kwon Gyhye Yoo |
| author_sort | Soyeon Hong |
| collection | DOAJ |
| description | Background: Osteoporosis is characterized by the microstructural depletion of bone tissue and decreased bone density, leading to an increased risk of fractures. <i>Cotoneaster wilsonii</i> Nakai, an endemic species of the Korean Peninsula, grows wild in Ulleungdo. In this study, we aimed to investigate the effects of <i>C. wilsonii</i> and its components on osteoporosis. Methods and Results: The alkaline phosphatase (ALP) activity of <i>C. wilsonii</i> extracts and fractions was evaluated in MC3T3-E1 pre-osteoblasts, and the <i>n</i>-hexane fraction (CWH) showed the best properties for ALP activity. The effects of the CWH on bone formation were assessed in MC3T3-E1 cells and ovariectomized mice. Biochemical assays and histological analyses focused on the signaling activation of osteoblast differentiation and osteogenic markers, such as ALP, collagen, and osterix. The CWH significantly activated TGF-β and Wnt signaling, enhancing osteoblast differentiation and bone matrix formation. Notably, CWH treatment improved micro-CT indices, such as femoral bone density, and restored serum osteocalcin levels compared to OVX controls. Conclusions: These results highlight the potential of the <i>C. wilsonii</i> Nakai <i>n</i>-hexane fraction as a promising therapeutic agent for managing osteoporosis. |
| format | Article |
| id | doaj-art-1489b23f282f4f47bed80a534f37e49a |
| institution | Kabale University |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-1489b23f282f4f47bed80a534f37e49a2025-01-24T13:45:08ZengMDPI AGPharmaceuticals1424-82472025-01-011814510.3390/ph18010045Exploring the Anti-Osteoporotic Effects of <i>n</i>-Hexane Fraction from <i>Cotoneaster wilsonii</i> Nakai: Activation of Runx2 and Osteoblast Differentiation In VivoSoyeon Hong0Hee Ju Lee1Da Seul Jung2Saruul Erdenebileg3Hoseong Hwang4Hak Cheol Kwon5Jaeyoung Kwon6Gyhye Yoo7Smart Farm Research Center, Korean Institute of Science and Technology (KIST), Gangneung 25451, Republic of KoreaCenter for Natural Product Systems Biology, Korean Institute of Science and Technology, Gangneung 25451, Republic of KoreaSmart Farm Research Center, Korean Institute of Science and Technology (KIST), Gangneung 25451, Republic of KoreaSmart Farm Research Center, Korean Institute of Science and Technology (KIST), Gangneung 25451, Republic of KoreaCenter for Natural Product Systems Biology, Korean Institute of Science and Technology, Gangneung 25451, Republic of KoreaCenter for Natural Product Systems Biology, Korean Institute of Science and Technology, Gangneung 25451, Republic of KoreaCenter for Natural Product Systems Biology, Korean Institute of Science and Technology, Gangneung 25451, Republic of KoreaSmart Farm Research Center, Korean Institute of Science and Technology (KIST), Gangneung 25451, Republic of KoreaBackground: Osteoporosis is characterized by the microstructural depletion of bone tissue and decreased bone density, leading to an increased risk of fractures. <i>Cotoneaster wilsonii</i> Nakai, an endemic species of the Korean Peninsula, grows wild in Ulleungdo. In this study, we aimed to investigate the effects of <i>C. wilsonii</i> and its components on osteoporosis. Methods and Results: The alkaline phosphatase (ALP) activity of <i>C. wilsonii</i> extracts and fractions was evaluated in MC3T3-E1 pre-osteoblasts, and the <i>n</i>-hexane fraction (CWH) showed the best properties for ALP activity. The effects of the CWH on bone formation were assessed in MC3T3-E1 cells and ovariectomized mice. Biochemical assays and histological analyses focused on the signaling activation of osteoblast differentiation and osteogenic markers, such as ALP, collagen, and osterix. The CWH significantly activated TGF-β and Wnt signaling, enhancing osteoblast differentiation and bone matrix formation. Notably, CWH treatment improved micro-CT indices, such as femoral bone density, and restored serum osteocalcin levels compared to OVX controls. Conclusions: These results highlight the potential of the <i>C. wilsonii</i> Nakai <i>n</i>-hexane fraction as a promising therapeutic agent for managing osteoporosis.https://www.mdpi.com/1424-8247/18/1/45<i>Cotoneaster wilsonii</i> Nakaibone formationosteoblast differentiationosteoporosisphytomedicineovariectomized mice |
| spellingShingle | Soyeon Hong Hee Ju Lee Da Seul Jung Saruul Erdenebileg Hoseong Hwang Hak Cheol Kwon Jaeyoung Kwon Gyhye Yoo Exploring the Anti-Osteoporotic Effects of <i>n</i>-Hexane Fraction from <i>Cotoneaster wilsonii</i> Nakai: Activation of Runx2 and Osteoblast Differentiation In Vivo Pharmaceuticals <i>Cotoneaster wilsonii</i> Nakai bone formation osteoblast differentiation osteoporosis phytomedicine ovariectomized mice |
| title | Exploring the Anti-Osteoporotic Effects of <i>n</i>-Hexane Fraction from <i>Cotoneaster wilsonii</i> Nakai: Activation of Runx2 and Osteoblast Differentiation In Vivo |
| title_full | Exploring the Anti-Osteoporotic Effects of <i>n</i>-Hexane Fraction from <i>Cotoneaster wilsonii</i> Nakai: Activation of Runx2 and Osteoblast Differentiation In Vivo |
| title_fullStr | Exploring the Anti-Osteoporotic Effects of <i>n</i>-Hexane Fraction from <i>Cotoneaster wilsonii</i> Nakai: Activation of Runx2 and Osteoblast Differentiation In Vivo |
| title_full_unstemmed | Exploring the Anti-Osteoporotic Effects of <i>n</i>-Hexane Fraction from <i>Cotoneaster wilsonii</i> Nakai: Activation of Runx2 and Osteoblast Differentiation In Vivo |
| title_short | Exploring the Anti-Osteoporotic Effects of <i>n</i>-Hexane Fraction from <i>Cotoneaster wilsonii</i> Nakai: Activation of Runx2 and Osteoblast Differentiation In Vivo |
| title_sort | exploring the anti osteoporotic effects of i n i hexane fraction from i cotoneaster wilsonii i nakai activation of runx2 and osteoblast differentiation in vivo |
| topic | <i>Cotoneaster wilsonii</i> Nakai bone formation osteoblast differentiation osteoporosis phytomedicine ovariectomized mice |
| url | https://www.mdpi.com/1424-8247/18/1/45 |
| work_keys_str_mv | AT soyeonhong exploringtheantiosteoporoticeffectsofinihexanefractionfromicotoneasterwilsoniiinakaiactivationofrunx2andosteoblastdifferentiationinvivo AT heejulee exploringtheantiosteoporoticeffectsofinihexanefractionfromicotoneasterwilsoniiinakaiactivationofrunx2andosteoblastdifferentiationinvivo AT daseuljung exploringtheantiosteoporoticeffectsofinihexanefractionfromicotoneasterwilsoniiinakaiactivationofrunx2andosteoblastdifferentiationinvivo AT saruulerdenebileg exploringtheantiosteoporoticeffectsofinihexanefractionfromicotoneasterwilsoniiinakaiactivationofrunx2andosteoblastdifferentiationinvivo AT hoseonghwang exploringtheantiosteoporoticeffectsofinihexanefractionfromicotoneasterwilsoniiinakaiactivationofrunx2andosteoblastdifferentiationinvivo AT hakcheolkwon exploringtheantiosteoporoticeffectsofinihexanefractionfromicotoneasterwilsoniiinakaiactivationofrunx2andosteoblastdifferentiationinvivo AT jaeyoungkwon exploringtheantiosteoporoticeffectsofinihexanefractionfromicotoneasterwilsoniiinakaiactivationofrunx2andosteoblastdifferentiationinvivo AT gyhyeyoo exploringtheantiosteoporoticeffectsofinihexanefractionfromicotoneasterwilsoniiinakaiactivationofrunx2andosteoblastdifferentiationinvivo |