Mycobacterium avium DNA extraction: Implications for NTM identification and amplicon sequencing

Mycobacterium avium DNA extraction: Implications for NTM identification and amplicon sequencing

This study evaluated six DNA (Deoxyribonucleic acid) extraction methods for Mycobacterium avium, focusing on cost, procedure time, yield, quality, DNA integrity, and suitability for amplicon-based sequencing. The extracted DNA was used in the GenoType® Mycobacterium Common Mycobacterium (CM) line pr...

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Bibliographic Details
Main Authors: Christoffel J. Opperman, Salim Ben Amor, Greshan Kisten, Brendon C. Mann, Janré Steyn, Sarishna Singh, Yonas Ghebrekristos, Robin Warren, Wynand Goosen
Format: Article
Language:English
Published: AOSIS 2025-05-01
Series:Southern African Journal of Infectious Diseases
Subjects:
mycobacterium avium
mycobacterium avium complex
nontuberculous mycobacteria
dna extraction
line probe assay
amplicon-based sequencing
genolyse version 1.0
Online Access:https://sajid.co.za/index.php/sajid/article/view/717
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