抵抗素对3T3-L1脂肪细胞葡萄糖摄取的影响

【目的】构建载有小鼠抵抗素(resistin)基因及其反义核酸的重组真核表达质粒,探讨抵抗素对3T3-L1脂肪细胞葡萄糖摄取的影响。【方法】用RT-PCR技术从小鼠脂肪组织扩增抵抗素基因cDNA,A/T克隆于pGEM-T载体,再亚克隆于pcDNA3.1(+)和pcDNA3.1(-)真核表达载体,构建载有小鼠抵抗素(resistin)基因及其反义核酸的重组真核表达质粒pcDNA3.1^(+)resistin和pcDNA3.1^(-)-resistin^(-)。将pcDNA3.1^(-)resistin^(-)转染小鼠3T3-L1脂肪细胞,通过G418筛选稳定表达的细胞株。3T3-L1脂肪细胞分为...

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Bibliographic Details
Main Authors: 李芳萍, 李焱, 傅玉如, 严励, 傅祖植
Format: Article
Language:zho
Published: Editorial Office of Journal of Sun Yat-sen University 2007-01-01
Series:Zhongshan Daxue xuebao. Yixue kexue ban
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Online Access:http://xuebaoyx.sysu.edu.cn/zh/article/43582255/
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