IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex
IL-7 signaling via IL-7Rα and common γ-chain (γc) is necessary for the development and homeostasis of T cells. Although the delicate mechanism in which IL-7Rα downregulation allows the homeostasis of T cell with limited IL-7 has been well known, the exact mechanism behind the interaction between IL-...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/9096829 |
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| author | Tae Sik Goh Yuna Jo Byunghyuk Lee Geona Kim Hyunju Hwang Eunhee Ko Seung Wan Kang Sae-Ock Oh Sun-Yong Baek Sik Yoon Jung Sub Lee Changwan Hong |
| author_facet | Tae Sik Goh Yuna Jo Byunghyuk Lee Geona Kim Hyunju Hwang Eunhee Ko Seung Wan Kang Sae-Ock Oh Sun-Yong Baek Sik Yoon Jung Sub Lee Changwan Hong |
| author_sort | Tae Sik Goh |
| collection | DOAJ |
| description | IL-7 signaling via IL-7Rα and common γ-chain (γc) is necessary for the development and homeostasis of T cells. Although the delicate mechanism in which IL-7Rα downregulation allows the homeostasis of T cell with limited IL-7 has been well known, the exact mechanism behind the interaction between IL-7Rα and γc in the absence or presence of IL-7 remains unclear. Additionally, we are still uncertain as to how only IL-7Rα is separately downregulated by the binding of IL-7 from the IL-7Rα/γc complex. We demonstrate here that 4G3, TUGm2, and 3E12 epitope masking of γc protein are induced in the presence of IL-7, indicating that the epitope alteration is induced by IL-7 binding to the preassembled receptor core. Moreover, the epitope masking of γc protein is inversely correlated with the expression of IL-7Rα upon IL-7 binding, implying that the structural alteration of γc might be involved in the regulation of IL-7Rα expression. The conformational change in γc upon IL-7 binding may contribute not only to forming the functional IL-7 signaling complex but also to optimally regulating the expression of IL-7Rα. |
| format | Article |
| id | doaj-art-13136ec1ba8b449d8f53dfb170558cf2 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-13136ec1ba8b449d8f53dfb170558cf22025-02-03T01:11:53ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/90968299096829IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling ComplexTae Sik Goh0Yuna Jo1Byunghyuk Lee2Geona Kim3Hyunju Hwang4Eunhee Ko5Seung Wan Kang6Sae-Ock Oh7Sun-Yong Baek8Sik Yoon9Jung Sub Lee10Changwan Hong11Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Orthopedic Surgery, Medical Research Institute, Pusan National University School of Medicine, Busan, Republic of KoreaDepartment of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of KoreaIL-7 signaling via IL-7Rα and common γ-chain (γc) is necessary for the development and homeostasis of T cells. Although the delicate mechanism in which IL-7Rα downregulation allows the homeostasis of T cell with limited IL-7 has been well known, the exact mechanism behind the interaction between IL-7Rα and γc in the absence or presence of IL-7 remains unclear. Additionally, we are still uncertain as to how only IL-7Rα is separately downregulated by the binding of IL-7 from the IL-7Rα/γc complex. We demonstrate here that 4G3, TUGm2, and 3E12 epitope masking of γc protein are induced in the presence of IL-7, indicating that the epitope alteration is induced by IL-7 binding to the preassembled receptor core. Moreover, the epitope masking of γc protein is inversely correlated with the expression of IL-7Rα upon IL-7 binding, implying that the structural alteration of γc might be involved in the regulation of IL-7Rα expression. The conformational change in γc upon IL-7 binding may contribute not only to forming the functional IL-7 signaling complex but also to optimally regulating the expression of IL-7Rα.http://dx.doi.org/10.1155/2017/9096829 |
| spellingShingle | Tae Sik Goh Yuna Jo Byunghyuk Lee Geona Kim Hyunju Hwang Eunhee Ko Seung Wan Kang Sae-Ock Oh Sun-Yong Baek Sik Yoon Jung Sub Lee Changwan Hong IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex Mediators of Inflammation |
| title | IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex |
| title_full | IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex |
| title_fullStr | IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex |
| title_full_unstemmed | IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex |
| title_short | IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex |
| title_sort | il 7 induces an epitope masking of γc protein in il 7 receptor signaling complex |
| url | http://dx.doi.org/10.1155/2017/9096829 |
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