Why Johnny can't clone: Common pitfalls and not so common solutions
The demand for cloned genes has increased incessantly over the past 32 years, but some who need recombinant plasmids struggle to produce them. While the pitfalls of traditional ligation-dependent cloning are non-trivial, most can be avoided with sufficient effort and attention to detail. Here, the c...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2015-09-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000114324 |
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