Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db Mouse
Type 2 diabetes (T2D) is characterized by β-cell dedifferentiation, but underlying mechanisms remain unclear. The purpose of the current study was to explore the mechanisms of β-cell dedifferentiation with and without long-term control of calorie intake. We used a diabetes mouse model (db/db) to ana...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/6035046 |
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| author | Chunjun Sheng Feng Li Ziwei Lin Manna Zhang Peng Yang Le Bu Hui Sheng Hong Li Shen Qu |
| author_facet | Chunjun Sheng Feng Li Ziwei Lin Manna Zhang Peng Yang Le Bu Hui Sheng Hong Li Shen Qu |
| author_sort | Chunjun Sheng |
| collection | DOAJ |
| description | Type 2 diabetes (T2D) is characterized by β-cell dedifferentiation, but underlying mechanisms remain unclear. The purpose of the current study was to explore the mechanisms of β-cell dedifferentiation with and without long-term control of calorie intake. We used a diabetes mouse model (db/db) to analyze the changes in the expression levels of β-cell-specific transcription factors (TFs) and functional factors with long-term caloric restriction (CR). Our results showed that chronic euglycemia was maintained in the db/db mice with long-term CR intervention, and β-cell dedifferentiation was significantly reduced. The expression of Glut2, Pdx1, and Nkx6.1 was reversed, while MafA expression was significantly increased with long-term CR. GLP-1 pathway was reactivated with long-term CR. Our work showed that the course of β-cell dedifferentiation can intervene by long-term control of calorie intake. Key β-cell-specific TFs and functional factors play important roles in maintaining β-cell differentiation. Targeting these factors could optimize T2D therapies. |
| format | Article |
| id | doaj-art-12112e5d292b4a1a8a46d2e42751fa85 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-12112e5d292b4a1a8a46d2e42751fa852025-02-03T01:01:18ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/60350466035046Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db MouseChunjun Sheng0Feng Li1Ziwei Lin2Manna Zhang3Peng Yang4Le Bu5Hui Sheng6Hong Li7Shen Qu8Department of Endocrinology and Metabolism, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology and Metabolism, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology and Metabolism, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology and Metabolism, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology and Metabolism, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology and Metabolism, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology and Metabolism, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology and Metabolism, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology and Metabolism, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaType 2 diabetes (T2D) is characterized by β-cell dedifferentiation, but underlying mechanisms remain unclear. The purpose of the current study was to explore the mechanisms of β-cell dedifferentiation with and without long-term control of calorie intake. We used a diabetes mouse model (db/db) to analyze the changes in the expression levels of β-cell-specific transcription factors (TFs) and functional factors with long-term caloric restriction (CR). Our results showed that chronic euglycemia was maintained in the db/db mice with long-term CR intervention, and β-cell dedifferentiation was significantly reduced. The expression of Glut2, Pdx1, and Nkx6.1 was reversed, while MafA expression was significantly increased with long-term CR. GLP-1 pathway was reactivated with long-term CR. Our work showed that the course of β-cell dedifferentiation can intervene by long-term control of calorie intake. Key β-cell-specific TFs and functional factors play important roles in maintaining β-cell differentiation. Targeting these factors could optimize T2D therapies.http://dx.doi.org/10.1155/2016/6035046 |
| spellingShingle | Chunjun Sheng Feng Li Ziwei Lin Manna Zhang Peng Yang Le Bu Hui Sheng Hong Li Shen Qu Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db Mouse Journal of Diabetes Research |
| title | Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db Mouse |
| title_full | Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db Mouse |
| title_fullStr | Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db Mouse |
| title_full_unstemmed | Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db Mouse |
| title_short | Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db Mouse |
| title_sort | reversibility of β cell specific transcript factors expression by long term caloric restriction in db db mouse |
| url | http://dx.doi.org/10.1155/2016/6035046 |
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