Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A
Streptococcus mutans produces 3 types of glucosyltransferases (GTFs), whose cooperative action is essential for cellular adhesion. The recombinase A (RecA) protein is required for homologous recombination. In our previous study, we isolated several strains with a smooth colony morphology and low GTF...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/405075 |
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| author | Satoko Inagaki Kazuyo Fujita Yukiko Takashima Kayoko Nagayama Arifah C. Ardin Yuki Matsumi Michiyo Matsumoto-Nakano |
| author_facet | Satoko Inagaki Kazuyo Fujita Yukiko Takashima Kayoko Nagayama Arifah C. Ardin Yuki Matsumi Michiyo Matsumoto-Nakano |
| author_sort | Satoko Inagaki |
| collection | DOAJ |
| description | Streptococcus mutans produces 3 types of glucosyltransferases (GTFs), whose cooperative action is essential for cellular adhesion. The recombinase A (RecA) protein is required for homologous recombination. In our previous study, we isolated several strains with a smooth colony morphology and low GTF activity, characteristics speculated to be derived from the GTF fusions. The purpose of the present study was to investigate the mechanism of those fusions. S. mutans strain MT8148 was grown in the presence of recombinant RecA (rRecA) protein, after which smooth colonies were isolated. The biological functions and sequences of the gtfB and gtfC genes of this as well as other clinical strains were determined. The sucrose-dependent adherence rates of those strains were reduced as compared to that of MT8148. Determination of the sequences of the gtfB and gtfC genes showed that an approximately 3500 bp region was deleted from the area between them. Furthermore, expression of the recA gene was elevated in those strains as compared to MT8148. These results suggest that RecA has an important role in fusions of gtfB and gtfC genes, leading to alteration of colony morphology and reduction in sucrose-dependent adhesion. |
| format | Article |
| id | doaj-art-11bea8fd058646b9bd49a1cf76c0a499 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-11bea8fd058646b9bd49a1cf76c0a4992025-02-03T01:31:31ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/405075405075Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase ASatoko Inagaki0Kazuyo Fujita1Yukiko Takashima2Kayoko Nagayama3Arifah C. Ardin4Yuki Matsumi5Michiyo Matsumoto-Nakano6Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1-8 Yamada-oka, Suita, Osaka 565-0871, JapanDepartment of Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1-8 Yamada-oka, Suita, Osaka 565-0871, JapanDepartment of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanDepartment of Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1-8 Yamada-oka, Suita, Osaka 565-0871, JapanDepartment of Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1-8 Yamada-oka, Suita, Osaka 565-0871, JapanDepartment of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanDepartment of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanStreptococcus mutans produces 3 types of glucosyltransferases (GTFs), whose cooperative action is essential for cellular adhesion. The recombinase A (RecA) protein is required for homologous recombination. In our previous study, we isolated several strains with a smooth colony morphology and low GTF activity, characteristics speculated to be derived from the GTF fusions. The purpose of the present study was to investigate the mechanism of those fusions. S. mutans strain MT8148 was grown in the presence of recombinant RecA (rRecA) protein, after which smooth colonies were isolated. The biological functions and sequences of the gtfB and gtfC genes of this as well as other clinical strains were determined. The sucrose-dependent adherence rates of those strains were reduced as compared to that of MT8148. Determination of the sequences of the gtfB and gtfC genes showed that an approximately 3500 bp region was deleted from the area between them. Furthermore, expression of the recA gene was elevated in those strains as compared to MT8148. These results suggest that RecA has an important role in fusions of gtfB and gtfC genes, leading to alteration of colony morphology and reduction in sucrose-dependent adhesion.http://dx.doi.org/10.1155/2013/405075 |
| spellingShingle | Satoko Inagaki Kazuyo Fujita Yukiko Takashima Kayoko Nagayama Arifah C. Ardin Yuki Matsumi Michiyo Matsumoto-Nakano Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A The Scientific World Journal |
| title | Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A |
| title_full | Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A |
| title_fullStr | Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A |
| title_full_unstemmed | Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A |
| title_short | Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A |
| title_sort | regulation of recombination between gtfb gtfc genes in streptococcus mutans by recombinase a |
| url | http://dx.doi.org/10.1155/2013/405075 |
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