Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench
Echinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homogeniz...
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| Format: | Article |
| Language: | English |
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Sciendo
2015-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2015-0036 |
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| author | Balciunaite Gabriele Juodsnukyte Jovita Savickas Arunas Ragazinskiene Ona Siatkute Luka Zvirblyte Gitana Mistiniene Edita Savickiene Nijole |
| author_facet | Balciunaite Gabriele Juodsnukyte Jovita Savickas Arunas Ragazinskiene Ona Siatkute Luka Zvirblyte Gitana Mistiniene Edita Savickiene Nijole |
| author_sort | Balciunaite Gabriele |
| collection | DOAJ |
| description | Echinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homogenization of roots with liquid nitrogen, extraction in 0.01 mol L-1 phosphate-buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. Total concentration of proteins was measured using the Bradford method, and evaluation of the molecular mass of proteins was accomplished by applying the SDS-PAGE gel electrophoresis. The Bradford assay revealed that the highest concentration of proteins in fractions collected after gel filtration chomatography was 4.66–6.07 mg mL-1. Glycoproteins, alkamides and polysaccharides in roots of Echinacea purpurea (L.) Moench are chemical compounds that are responsible for their immunomodulatory properties. However, information about the difference of protein contents in fresh and dried roots of E. purpurea is insufficient. |
| format | Article |
| id | doaj-art-11a5a130f53b49afbd33629175d1a61d |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-11a5a130f53b49afbd33629175d1a61d2025-02-02T09:35:17ZengSciendoActa Pharmaceutica1846-95582015-12-0165447347910.1515/acph-2015-0036acph-2015-0036Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) MoenchBalciunaite Gabriele0Juodsnukyte Jovita1Savickas Arunas2Ragazinskiene Ona3Siatkute Luka4Zvirblyte Gitana5Mistiniene Edita6Savickiene Nijole7Department of Pharmacognosy Lithuanian University of Health Sciences Kaunas, LithuaniaDepartment of Pharmacognosy Lithuanian University of Health Sciences Kaunas, LithuaniaDepartment of Social Pharmacy and Drug Technology, Lithuanian University of Health Sciences, Kaunas, Lithuania 4Kaunas Botanical Garden of Vytautas Magnus University Kaunas, LithuaniaKaunas Botanical Garden of Vytautas Magnus University Kaunas, LithuaniaDepartment of Pharmacognosy Lithuanian University of Health Sciences Kaunas, LithuaniaInstitute of Biotechnology Vilnius University, Vilnius, LithuaniaInstitute of Biotechnology Vilnius University, Vilnius, LithuaniaDepartment of Pharmacognosy Lithuanian University of Health Sciences Kaunas, LithuaniaEchinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homogenization of roots with liquid nitrogen, extraction in 0.01 mol L-1 phosphate-buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. Total concentration of proteins was measured using the Bradford method, and evaluation of the molecular mass of proteins was accomplished by applying the SDS-PAGE gel electrophoresis. The Bradford assay revealed that the highest concentration of proteins in fractions collected after gel filtration chomatography was 4.66–6.07 mg mL-1. Glycoproteins, alkamides and polysaccharides in roots of Echinacea purpurea (L.) Moench are chemical compounds that are responsible for their immunomodulatory properties. However, information about the difference of protein contents in fresh and dried roots of E. purpurea is insufficient.https://doi.org/10.1515/acph-2015-0036echinacea purpurea (l.) moenchproteinsgel filtration chromatographysds-page |
| spellingShingle | Balciunaite Gabriele Juodsnukyte Jovita Savickas Arunas Ragazinskiene Ona Siatkute Luka Zvirblyte Gitana Mistiniene Edita Savickiene Nijole Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench Acta Pharmaceutica echinacea purpurea (l.) moench proteins gel filtration chromatography sds-page |
| title | Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench |
| title_full | Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench |
| title_fullStr | Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench |
| title_full_unstemmed | Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench |
| title_short | Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench |
| title_sort | fractionation and evaluation of proteins in roots of echinacea purpurea l moench |
| topic | echinacea purpurea (l.) moench proteins gel filtration chromatography sds-page |
| url | https://doi.org/10.1515/acph-2015-0036 |
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