Comparative Study of Antibacterial Activity of Cistus ladanifer L. Leaves Extracted by Ultrasound-Assisted Extraction and Maceration
Addressing infectious diseases poses a significant healthcare challenge due to the growing resistance of microorganisms to antibiotics. Consequently, extensive research efforts have been dedicated to identifying alternative molecules to antibiotics that can combat these infectious diseases. Notably...
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| Format: | Article |
| Language: | English |
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HH Publisher
2023-12-01
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| Series: | Progress in Microbes and Molecular Biology |
| Online Access: | https://journals.hh-publisher.com/index.php/pmmb/article/view/925 |
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| author | Houda Chtibi Kaoutar Harboul Taofiq Benali Abdelhakim Bouyahya Khabbach Abdelamjid Said Louahlia Jactty Chew Long Chiau Ming Khalil Hammani |
| author_facet | Houda Chtibi Kaoutar Harboul Taofiq Benali Abdelhakim Bouyahya Khabbach Abdelamjid Said Louahlia Jactty Chew Long Chiau Ming Khalil Hammani |
| author_sort | Houda Chtibi |
| collection | DOAJ |
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Addressing infectious diseases poses a significant healthcare challenge due to the growing resistance of microorganisms to antibiotics. Consequently, extensive research efforts have been dedicated to identifying alternative molecules to antibiotics that can combat these infectious diseases. Notably, a wealth of bioactive compounds has been discovered in medicinal and aromatic plants, and their potential utility in the development of treatments for infectious diseases has been well-established. In this study, we employed two extraction methods, namely maceration and ultrasound-assisted extraction (UAE), and four different solvents with varying polarities to isolate bioactive compounds from Cistus ladanifer L. Subsequently, quantification of polyphenols was determined in all the extracted samples. Following this, we conducted both qualitative and quantitative assessments of the antibacterial activity of these extracts. Moreover, we delved into the mechanism of action of the most potent extract using scanning electron microscopy. The results revealed that methanol was the best solvent for extracting bioactive molecules and that UAE had a high extraction efficiency since it gave a higher yield of total polyphenols than the one acquired by maceration. Regarding the results of the disk diffusion assay, the extracts that were prepared by UAE were more effective against our bacterial strains than those extracted by maceration. Electron micrographs of damaged cells revealed that the methanolic extract obtained by UAE affected the integrity of the cell membrane. These outcomes have underscored the presence of active compounds within Cistus ladanifer L. that could serve as potential alternatives to antibiotics against some of the most pathogenic bacterial species.
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| format | Article |
| id | doaj-art-112f3ca2364745b8b636babd4ff9a9cf |
| institution | Kabale University |
| issn | 2637-1049 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | HH Publisher |
| record_format | Article |
| series | Progress in Microbes and Molecular Biology |
| spelling | doaj-art-112f3ca2364745b8b636babd4ff9a9cf2025-02-04T08:39:41ZengHH PublisherProgress in Microbes and Molecular Biology2637-10492023-12-016110.36877/pmmb.a0000394Comparative Study of Antibacterial Activity of Cistus ladanifer L. Leaves Extracted by Ultrasound-Assisted Extraction and MacerationHouda ChtibiKaoutar HarboulTaofiq BenaliAbdelhakim BouyahyaKhabbach AbdelamjidSaid LouahliaJactty ChewLong Chiau MingKhalil Hammani Addressing infectious diseases poses a significant healthcare challenge due to the growing resistance of microorganisms to antibiotics. Consequently, extensive research efforts have been dedicated to identifying alternative molecules to antibiotics that can combat these infectious diseases. Notably, a wealth of bioactive compounds has been discovered in medicinal and aromatic plants, and their potential utility in the development of treatments for infectious diseases has been well-established. In this study, we employed two extraction methods, namely maceration and ultrasound-assisted extraction (UAE), and four different solvents with varying polarities to isolate bioactive compounds from Cistus ladanifer L. Subsequently, quantification of polyphenols was determined in all the extracted samples. Following this, we conducted both qualitative and quantitative assessments of the antibacterial activity of these extracts. Moreover, we delved into the mechanism of action of the most potent extract using scanning electron microscopy. The results revealed that methanol was the best solvent for extracting bioactive molecules and that UAE had a high extraction efficiency since it gave a higher yield of total polyphenols than the one acquired by maceration. Regarding the results of the disk diffusion assay, the extracts that were prepared by UAE were more effective against our bacterial strains than those extracted by maceration. Electron micrographs of damaged cells revealed that the methanolic extract obtained by UAE affected the integrity of the cell membrane. These outcomes have underscored the presence of active compounds within Cistus ladanifer L. that could serve as potential alternatives to antibiotics against some of the most pathogenic bacterial species. https://journals.hh-publisher.com/index.php/pmmb/article/view/925 |
| spellingShingle | Houda Chtibi Kaoutar Harboul Taofiq Benali Abdelhakim Bouyahya Khabbach Abdelamjid Said Louahlia Jactty Chew Long Chiau Ming Khalil Hammani Comparative Study of Antibacterial Activity of Cistus ladanifer L. Leaves Extracted by Ultrasound-Assisted Extraction and Maceration Progress in Microbes and Molecular Biology |
| title | Comparative Study of Antibacterial Activity of Cistus ladanifer L. Leaves Extracted by Ultrasound-Assisted Extraction and Maceration |
| title_full | Comparative Study of Antibacterial Activity of Cistus ladanifer L. Leaves Extracted by Ultrasound-Assisted Extraction and Maceration |
| title_fullStr | Comparative Study of Antibacterial Activity of Cistus ladanifer L. Leaves Extracted by Ultrasound-Assisted Extraction and Maceration |
| title_full_unstemmed | Comparative Study of Antibacterial Activity of Cistus ladanifer L. Leaves Extracted by Ultrasound-Assisted Extraction and Maceration |
| title_short | Comparative Study of Antibacterial Activity of Cistus ladanifer L. Leaves Extracted by Ultrasound-Assisted Extraction and Maceration |
| title_sort | comparative study of antibacterial activity of cistus ladanifer l leaves extracted by ultrasound assisted extraction and maceration |
| url | https://journals.hh-publisher.com/index.php/pmmb/article/view/925 |
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