Surface topography modulates initial platelet adhesion to titanium substrata
The clinical success of implanted biomaterials such as dental implants is largely determined by the molecular signaling that occurs at the tissue-implant interface. The modification of surface topography is a widely-employed strategy for optimizing tissue integration with dental implants. However, l...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-07-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426824000319 |
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| _version_ | 1832589957909184512 |
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| author | Cecilia Yan Guo Raymond Mo Hugh Kim |
| author_facet | Cecilia Yan Guo Raymond Mo Hugh Kim |
| author_sort | Cecilia Yan Guo |
| collection | DOAJ |
| description | The clinical success of implanted biomaterials such as dental implants is largely determined by the molecular signaling that occurs at the tissue-implant interface. The modification of surface topography is a widely-employed strategy for optimizing tissue integration with dental implants. However, little is known regarding the direct, cellular-level effects of substratum topography on platelet signaling and adhesion, despite these cells being the first to encounter the implant surface during surgical placement. Here we compared platelet adhesion and secretion on four (4) different titanium surfaces, notably, the modifications applied to commercially available dental implants: smooth (S) titanium; acid-etched (AE), sandblasted (SB) and a combined acid-etching/sandblasting procedure (SLA). Platelets were isolated from human blood, washed, and seeded on to the 4 test surfaces; platelet adhesion was quantified by microscopy. In addition, the secretion of critical molecules stored in platelet granules (platelet factor 4, PF4; soluble P-selectin, sCD62P; transforming growth factor-beta1, TGF-β1; platelet-derived growth factor-AB, PDGF-AB) was measured by enzyme-linked immunosorbent assay (ELISA) analysis of the supernatants. There was greater platelet adhesion to the rougher AE and SB surfaces, however, the concentration of the secreted growth factors was comparable on all surfaces. We conclude that while surface topography can be engineered to modulate initial platelet adhesion, granule secretion is likely regulated as a separate and independent process. |
| format | Article |
| id | doaj-art-110dddfde4e44d5b8303ee09f67cda65 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-110dddfde4e44d5b8303ee09f67cda652025-01-24T04:45:01ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682024-07-01144471477Surface topography modulates initial platelet adhesion to titanium substrataCecilia Yan Guo0Raymond Mo1Hugh Kim2Centre for Blood Research, University of British Columbia, Life Sciences Institute, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3; Department of Oral Biological and Medical Sciences, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, Canada V6T 1Z3Centre for Blood Research, University of British Columbia, Life Sciences Institute, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3; Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3Centre for Blood Research, University of British Columbia, Life Sciences Institute, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3; Department of Oral Biological and Medical Sciences, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, Canada V6T 1Z3; Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3; Corresponding author. UBC Centre for Blood Research 2350 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada.The clinical success of implanted biomaterials such as dental implants is largely determined by the molecular signaling that occurs at the tissue-implant interface. The modification of surface topography is a widely-employed strategy for optimizing tissue integration with dental implants. However, little is known regarding the direct, cellular-level effects of substratum topography on platelet signaling and adhesion, despite these cells being the first to encounter the implant surface during surgical placement. Here we compared platelet adhesion and secretion on four (4) different titanium surfaces, notably, the modifications applied to commercially available dental implants: smooth (S) titanium; acid-etched (AE), sandblasted (SB) and a combined acid-etching/sandblasting procedure (SLA). Platelets were isolated from human blood, washed, and seeded on to the 4 test surfaces; platelet adhesion was quantified by microscopy. In addition, the secretion of critical molecules stored in platelet granules (platelet factor 4, PF4; soluble P-selectin, sCD62P; transforming growth factor-beta1, TGF-β1; platelet-derived growth factor-AB, PDGF-AB) was measured by enzyme-linked immunosorbent assay (ELISA) analysis of the supernatants. There was greater platelet adhesion to the rougher AE and SB surfaces, however, the concentration of the secreted growth factors was comparable on all surfaces. We conclude that while surface topography can be engineered to modulate initial platelet adhesion, granule secretion is likely regulated as a separate and independent process.http://www.sciencedirect.com/science/article/pii/S2212426824000319PlateletsImplantSurface topographyCell adhesion |
| spellingShingle | Cecilia Yan Guo Raymond Mo Hugh Kim Surface topography modulates initial platelet adhesion to titanium substrata Journal of Oral Biology and Craniofacial Research Platelets Implant Surface topography Cell adhesion |
| title | Surface topography modulates initial platelet adhesion to titanium substrata |
| title_full | Surface topography modulates initial platelet adhesion to titanium substrata |
| title_fullStr | Surface topography modulates initial platelet adhesion to titanium substrata |
| title_full_unstemmed | Surface topography modulates initial platelet adhesion to titanium substrata |
| title_short | Surface topography modulates initial platelet adhesion to titanium substrata |
| title_sort | surface topography modulates initial platelet adhesion to titanium substrata |
| topic | Platelets Implant Surface topography Cell adhesion |
| url | http://www.sciencedirect.com/science/article/pii/S2212426824000319 |
| work_keys_str_mv | AT ceciliayanguo surfacetopographymodulatesinitialplateletadhesiontotitaniumsubstrata AT raymondmo surfacetopographymodulatesinitialplateletadhesiontotitaniumsubstrata AT hughkim surfacetopographymodulatesinitialplateletadhesiontotitaniumsubstrata |