Synergistic Effects of SDS and H2O2 Combinations on Tracheal Scaffold Development: An In Vitro Study Using Goat Trachea
Currently, a tissue-engineered trachea has been popularly used as a biological graft for tracheal replacement in severe respiratory diseases. In the development of tissue-engineered tracheal scaffolds, in vitro studies play a crucial role in allowing researchers to evaluate the efficacy and safety o...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2024/6635565 |
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| _version_ | 1832558566872973312 |
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| author | Dhihintia Jiwangga Ferdiansyah Mahyudin Gondo Mastutik Estya Nadya Meitavany null Juliana Priangga Adi Wiratama |
| author_facet | Dhihintia Jiwangga Ferdiansyah Mahyudin Gondo Mastutik Estya Nadya Meitavany null Juliana Priangga Adi Wiratama |
| author_sort | Dhihintia Jiwangga |
| collection | DOAJ |
| description | Currently, a tissue-engineered trachea has been popularly used as a biological graft for tracheal replacement in severe respiratory diseases. In the development of tissue-engineered tracheal scaffolds, in vitro studies play a crucial role in allowing researchers to evaluate the efficacy and safety of scaffold designs and fabrication techniques before progressing to in vivo or clinical trials. This research involved the decellularization of goat trachea using SDS, H2O2, and their combinations. Various quantitative and qualitative assessments were performed, including histological analysis, immunohistochemistry, and biomechanical testing. Hematoxylin and eosin staining evaluated the cellular content, while safranin O-fast green and Masson’s trichrome staining assessed glycosaminoglycan content and collagen distribution, respectively. The immunohistochemical analysis focused on detecting MHC-1 antigen presence. Tensile strength measurements were conducted to evaluate the biomechanical properties of the decellularized scaffolds. The results demonstrated that the combination of SDS and H2O2 for goat tracheal decellularization yielded scaffolds with minimal cellular remnants, low toxicity, preserved ECM, and high tensile strength and elasticity. This method holds promise for developing functional tracheal scaffolds to address severe respiratory diseases effectively. |
| format | Article |
| id | doaj-art-10d10e5fd3054c48959f2891f8dd1ee1 |
| institution | Kabale University |
| issn | 1687-8795 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-10d10e5fd3054c48959f2891f8dd1ee12025-02-03T01:32:00ZengWileyInternational Journal of Biomaterials1687-87952024-01-01202410.1155/2024/6635565Synergistic Effects of SDS and H2O2 Combinations on Tracheal Scaffold Development: An In Vitro Study Using Goat TracheaDhihintia Jiwangga0Ferdiansyah Mahyudin1Gondo Mastutik2Estya Nadya Meitavany3null Juliana4Priangga Adi Wiratama5Doctoral Program of Medical ScienceDepartment of Orthopaedic and TraumatologyDepartment of Anatomic PathologySchool of Biomedical Engineering and Imaging SciencesFaculty of MedicineDepartment of Anatomic PathologyCurrently, a tissue-engineered trachea has been popularly used as a biological graft for tracheal replacement in severe respiratory diseases. In the development of tissue-engineered tracheal scaffolds, in vitro studies play a crucial role in allowing researchers to evaluate the efficacy and safety of scaffold designs and fabrication techniques before progressing to in vivo or clinical trials. This research involved the decellularization of goat trachea using SDS, H2O2, and their combinations. Various quantitative and qualitative assessments were performed, including histological analysis, immunohistochemistry, and biomechanical testing. Hematoxylin and eosin staining evaluated the cellular content, while safranin O-fast green and Masson’s trichrome staining assessed glycosaminoglycan content and collagen distribution, respectively. The immunohistochemical analysis focused on detecting MHC-1 antigen presence. Tensile strength measurements were conducted to evaluate the biomechanical properties of the decellularized scaffolds. The results demonstrated that the combination of SDS and H2O2 for goat tracheal decellularization yielded scaffolds with minimal cellular remnants, low toxicity, preserved ECM, and high tensile strength and elasticity. This method holds promise for developing functional tracheal scaffolds to address severe respiratory diseases effectively.http://dx.doi.org/10.1155/2024/6635565 |
| spellingShingle | Dhihintia Jiwangga Ferdiansyah Mahyudin Gondo Mastutik Estya Nadya Meitavany null Juliana Priangga Adi Wiratama Synergistic Effects of SDS and H2O2 Combinations on Tracheal Scaffold Development: An In Vitro Study Using Goat Trachea International Journal of Biomaterials |
| title | Synergistic Effects of SDS and H2O2 Combinations on Tracheal Scaffold Development: An In Vitro Study Using Goat Trachea |
| title_full | Synergistic Effects of SDS and H2O2 Combinations on Tracheal Scaffold Development: An In Vitro Study Using Goat Trachea |
| title_fullStr | Synergistic Effects of SDS and H2O2 Combinations on Tracheal Scaffold Development: An In Vitro Study Using Goat Trachea |
| title_full_unstemmed | Synergistic Effects of SDS and H2O2 Combinations on Tracheal Scaffold Development: An In Vitro Study Using Goat Trachea |
| title_short | Synergistic Effects of SDS and H2O2 Combinations on Tracheal Scaffold Development: An In Vitro Study Using Goat Trachea |
| title_sort | synergistic effects of sds and h2o2 combinations on tracheal scaffold development an in vitro study using goat trachea |
| url | http://dx.doi.org/10.1155/2024/6635565 |
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