Diethylcarbamazine Reduces Chronic Inflammation and Fibrosis in Carbon Tetrachloride- (CCl4-) Induced Liver Injury in Mice
This study investigated the anti-inflammatory effects of DEC on the CCl4-induced hepatotoxicity in C57BL/6 mice. Chronic inflammation was induced by i.p. administration of CCl4 0.5 μL/g of body weight through two injections a week for 6 weeks. DEC (50 mg/kg) was administered by gavage for 12 days be...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/696383 |
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| author | Sura Wanessa Santos Rocha Maria Eduarda Rocha de França Gabriel Barros Rodrigues Karla Patrícia Sousa Barbosa Ana Karolina Santana Nunes André Filipe Pastor Anne Gabrielle Vasconcelos Oliveira Wilma Helena Oliveira Rayana Leal Almeida Luna Christina Alves Peixoto |
| author_facet | Sura Wanessa Santos Rocha Maria Eduarda Rocha de França Gabriel Barros Rodrigues Karla Patrícia Sousa Barbosa Ana Karolina Santana Nunes André Filipe Pastor Anne Gabrielle Vasconcelos Oliveira Wilma Helena Oliveira Rayana Leal Almeida Luna Christina Alves Peixoto |
| author_sort | Sura Wanessa Santos Rocha |
| collection | DOAJ |
| description | This study investigated the anti-inflammatory effects of DEC on the CCl4-induced hepatotoxicity in C57BL/6 mice. Chronic inflammation was induced by i.p. administration of CCl4 0.5 μL/g of body weight through two injections a week for 6 weeks. DEC (50 mg/kg) was administered by gavage for 12 days before finishing the CCl4 induction. Histological analyses of the DEC-treated group exhibited reduced inflammatory process and prevented liver necrosis and fibrosis. Immunohistochemical and immunofluorescence analyses of the DEC-treated group showed reduced COX-2, IL1β, MDA, TGF-β, and αSMA immunopositivity, besides exhibiting decreased IL1β, COX-2, NFκB, IFNγ, and TGFβ expressions in the western blot analysis. The DEC group enhanced significantly the IL-10 expression. The reduction of hepatic injury in the DEC-treated group was confirmed by the COX-2 and iNOS mRNA expression levels. Based on the results of the present study, DEC can be used as a potential anti-inflammatory drug for chronic hepatic inflammation. |
| format | Article |
| id | doaj-art-108a0b36a6494955acaabd5a931864b2 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-108a0b36a6494955acaabd5a931864b22025-02-03T01:31:16ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/696383696383Diethylcarbamazine Reduces Chronic Inflammation and Fibrosis in Carbon Tetrachloride- (CCl4-) Induced Liver Injury in MiceSura Wanessa Santos Rocha0Maria Eduarda Rocha de França1Gabriel Barros Rodrigues2Karla Patrícia Sousa Barbosa3Ana Karolina Santana Nunes4André Filipe Pastor5Anne Gabrielle Vasconcelos Oliveira6Wilma Helena Oliveira7Rayana Leal Almeida Luna8Christina Alves Peixoto9Laboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, BrazilLaboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, BrazilLaboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, BrazilLaboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, BrazilLaboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, BrazilLaboratory of Virology and Experimental Therapy, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, BrazilLaboratory of Microscopy and Microanalisis, Northeastern Center for Strategic Technologies (CETENE), Avenida Professor Luiz Freire, Cidade Universitária, 50740-540 Recife, PE, BrazilLaboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, BrazilLaboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, BrazilLaboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, BrazilThis study investigated the anti-inflammatory effects of DEC on the CCl4-induced hepatotoxicity in C57BL/6 mice. Chronic inflammation was induced by i.p. administration of CCl4 0.5 μL/g of body weight through two injections a week for 6 weeks. DEC (50 mg/kg) was administered by gavage for 12 days before finishing the CCl4 induction. Histological analyses of the DEC-treated group exhibited reduced inflammatory process and prevented liver necrosis and fibrosis. Immunohistochemical and immunofluorescence analyses of the DEC-treated group showed reduced COX-2, IL1β, MDA, TGF-β, and αSMA immunopositivity, besides exhibiting decreased IL1β, COX-2, NFκB, IFNγ, and TGFβ expressions in the western blot analysis. The DEC group enhanced significantly the IL-10 expression. The reduction of hepatic injury in the DEC-treated group was confirmed by the COX-2 and iNOS mRNA expression levels. Based on the results of the present study, DEC can be used as a potential anti-inflammatory drug for chronic hepatic inflammation.http://dx.doi.org/10.1155/2014/696383 |
| spellingShingle | Sura Wanessa Santos Rocha Maria Eduarda Rocha de França Gabriel Barros Rodrigues Karla Patrícia Sousa Barbosa Ana Karolina Santana Nunes André Filipe Pastor Anne Gabrielle Vasconcelos Oliveira Wilma Helena Oliveira Rayana Leal Almeida Luna Christina Alves Peixoto Diethylcarbamazine Reduces Chronic Inflammation and Fibrosis in Carbon Tetrachloride- (CCl4-) Induced Liver Injury in Mice Mediators of Inflammation |
| title | Diethylcarbamazine Reduces Chronic Inflammation and Fibrosis in Carbon Tetrachloride- (CCl4-) Induced Liver Injury in Mice |
| title_full | Diethylcarbamazine Reduces Chronic Inflammation and Fibrosis in Carbon Tetrachloride- (CCl4-) Induced Liver Injury in Mice |
| title_fullStr | Diethylcarbamazine Reduces Chronic Inflammation and Fibrosis in Carbon Tetrachloride- (CCl4-) Induced Liver Injury in Mice |
| title_full_unstemmed | Diethylcarbamazine Reduces Chronic Inflammation and Fibrosis in Carbon Tetrachloride- (CCl4-) Induced Liver Injury in Mice |
| title_short | Diethylcarbamazine Reduces Chronic Inflammation and Fibrosis in Carbon Tetrachloride- (CCl4-) Induced Liver Injury in Mice |
| title_sort | diethylcarbamazine reduces chronic inflammation and fibrosis in carbon tetrachloride ccl4 induced liver injury in mice |
| url | http://dx.doi.org/10.1155/2014/696383 |
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