Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study
Metabolic hormones have been associated with reproductive function modulation. Thus, the aim of this study was: (i) to characterize the immunolocalization, mRNA and protein levels of leptin (LEP), Ghrelin (GHR) and respective receptors LEPR and Ghr-R1A, throughout luteal phase; and (ii) to evaluate...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/682193 |
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| author | António Galvão Angela Tramontano Maria Rosa Rebordão Ana Amaral Pedro Pinto Bravo Anna Szóstek Dariusz Skarzynski Antonio Mollo Graça Ferreira-Dias |
| author_facet | António Galvão Angela Tramontano Maria Rosa Rebordão Ana Amaral Pedro Pinto Bravo Anna Szóstek Dariusz Skarzynski Antonio Mollo Graça Ferreira-Dias |
| author_sort | António Galvão |
| collection | DOAJ |
| description | Metabolic hormones have been associated with reproductive function modulation. Thus, the aim of this study was: (i) to characterize the immunolocalization, mRNA and protein levels of leptin (LEP), Ghrelin (GHR) and respective receptors LEPR and Ghr-R1A, throughout luteal phase; and (ii) to evaluate the role of LEP and GHR on progesterone (P4), prostaglandin (PG) E2 and PGF2α, nitric oxide (nitrite), tumor necrosis factor-α (TNF); macrophage migration inhibitory factor (MIF) secretion, and on angiogenic activity (BAEC proliferation), in equine corpus luteum (CL) from early and mid-luteal stages. LEPR expression was decreased in late CL, while GHR/Ghr-R1A system was increased in the same stage. Regarding secretory activity, GHR decreased P4 in early CL, but increased PGF2α, nitrite and TNF in mid CL. Conversely, LEP increased P4, PGE2, angiogenic activity, MIF, TNF and nitrite during early CL, in a dose-dependent manner. The in vitro effect of LEP on secretory activity was reverted by GHR, when both factors acted together. The present results evidence the presence of LEP and GHR systems in the equine CL. Moreover, we suggest that LEP and GHR play opposing roles in equine CL regulation, with LEP supporting luteal establishment and GHR promoting luteal regression. Finally, a dose-dependent luteotrophic effect of LEP was demonstrated. |
| format | Article |
| id | doaj-art-105d04edd4a1403c814b9ff2c00e1c7a |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-105d04edd4a1403c814b9ff2c00e1c7a2025-02-03T05:58:59ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/682193682193Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro StudyAntónio Galvão0Angela Tramontano1Maria Rosa Rebordão2Ana Amaral3Pedro Pinto Bravo4Anna Szóstek5Dariusz Skarzynski6Antonio Mollo7Graça Ferreira-Dias8C.I.I.S.A., Faculty of Veterinary Medicine, University of Lisbon, PortugalDepartment of Animal Medicine, Production and Health (MAPS), Faculty of Veterinary Medicine, University of Padua, Viale dell’Università 16, 35020 Legnaro, Padua, ItalyC.I.I.S.A., Faculty of Veterinary Medicine, University of Lisbon, PortugalC.I.I.S.A., Faculty of Veterinary Medicine, University of Lisbon, PortugalC.I.I.S.A., Faculty of Veterinary Medicine, University of Lisbon, PortugalInstitute of Animal Reproduction and Food Research of PAS, Olsztyn, PolandInstitute of Animal Reproduction and Food Research of PAS, Olsztyn, PolandDepartment of Animal Medicine, Production and Health (MAPS), Faculty of Veterinary Medicine, University of Padua, Viale dell’Università 16, 35020 Legnaro, Padua, ItalyC.I.I.S.A., Faculty of Veterinary Medicine, University of Lisbon, PortugalMetabolic hormones have been associated with reproductive function modulation. Thus, the aim of this study was: (i) to characterize the immunolocalization, mRNA and protein levels of leptin (LEP), Ghrelin (GHR) and respective receptors LEPR and Ghr-R1A, throughout luteal phase; and (ii) to evaluate the role of LEP and GHR on progesterone (P4), prostaglandin (PG) E2 and PGF2α, nitric oxide (nitrite), tumor necrosis factor-α (TNF); macrophage migration inhibitory factor (MIF) secretion, and on angiogenic activity (BAEC proliferation), in equine corpus luteum (CL) from early and mid-luteal stages. LEPR expression was decreased in late CL, while GHR/Ghr-R1A system was increased in the same stage. Regarding secretory activity, GHR decreased P4 in early CL, but increased PGF2α, nitrite and TNF in mid CL. Conversely, LEP increased P4, PGE2, angiogenic activity, MIF, TNF and nitrite during early CL, in a dose-dependent manner. The in vitro effect of LEP on secretory activity was reverted by GHR, when both factors acted together. The present results evidence the presence of LEP and GHR systems in the equine CL. Moreover, we suggest that LEP and GHR play opposing roles in equine CL regulation, with LEP supporting luteal establishment and GHR promoting luteal regression. Finally, a dose-dependent luteotrophic effect of LEP was demonstrated.http://dx.doi.org/10.1155/2014/682193 |
| spellingShingle | António Galvão Angela Tramontano Maria Rosa Rebordão Ana Amaral Pedro Pinto Bravo Anna Szóstek Dariusz Skarzynski Antonio Mollo Graça Ferreira-Dias Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study Mediators of Inflammation |
| title | Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study |
| title_full | Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study |
| title_fullStr | Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study |
| title_full_unstemmed | Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study |
| title_short | Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study |
| title_sort | opposing roles of leptin and ghrelin in the equine corpus luteum regulation an in vitro study |
| url | http://dx.doi.org/10.1155/2014/682193 |
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