Dietary Solid-state-fermentation product of Bacillus velezensis T23 alleviate hepatic steatosis, oxidative stress, gut barrier damage, and microbiota dysbiosis in juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus)
The application of commensal probiotic bacteria is one of the preferable green feed additives for antibiotic substitution in aquaculture, while the application methods and detailed action mechanisms of commensal bacteria isolated from aquatic animals are not consistent with normal mammalian-derived...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006112 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832087454005329920 |
|---|---|
| author | Qiang Hao Rolf Erik Olsen Einar Ringø Qianwen Ding Tsegay Teame Yuanyuan Yao Chao Ran Yalin Yang Zhen Zhang Zhigang Zhou |
| author_facet | Qiang Hao Rolf Erik Olsen Einar Ringø Qianwen Ding Tsegay Teame Yuanyuan Yao Chao Ran Yalin Yang Zhen Zhang Zhigang Zhou |
| author_sort | Qiang Hao |
| collection | DOAJ |
| description | The application of commensal probiotic bacteria is one of the preferable green feed additives for antibiotic substitution in aquaculture, while the application methods and detailed action mechanisms of commensal bacteria isolated from aquatic animals are not consistent with normal mammalian-derived probiotics. The purpose of this study was to evaluate the effects of solid-state fermentation product of autochthonous Bacillus velezensis T23 on improving hepatic steatosis, liver antioxidation capacity, gut injury and gut microbiota profile of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). The T23 probiotic was added to the basal diet at a level of 0.00, 0.05, 0.1, 0.2 and 0.3 g/kg to develop five experimental diets, respectively. After 4 weeks of the feeding trial, the results showed that T23 supplementation notably reduced the hepatocytes vacuolization and the content of liver TAGs by downregulated the mRNA expression of the lipogenesis gene (fas), while upregulated the mRNA expression of lipid degradation genes (cpt1 and pparα) (p < 0.05). Compared with the control, the 0.3T23 group showed significantly upregulated expression levels of anti-inflammatory cytokine genes (tgf-β and il-10) in the liver of the fish (p < 0.05). Besides, the activities of antioxidase SOD and CAT were significantly upregulated in T23 treatment groups, compared with the control (p < 0.05). Fish groups fed with 0.2 g/kg and 0.3 g/kg T23 supplemented diet also exhibited significantly lower intestinal injury and intestinal inflammation via upregulating the expression of tight junction protein gene claudin, anti-inflammatory factor gene il-10 (p < 0.05). However, markedly downregulation of the mRNA level of pro-inflammatory cytokine il-1β (p < 0.05) was observed in 0.05 and 0.3T23 groups, compared with the control. 16S rRNA sequencing analysis data on gut microbiota indicated that supplementation of T23 at lower levels (0.05, 0.1 and 0.2) resulted in a remarkable elevation of the abundance of phylum Firmicutes and reduced the abundance of Proteobacteria, and the ratio of (Firmicutes +Bacteroidota +Fusobacteria) /Proteobacteria. On the contrary, feeding with 0.3T23 negatively affects the gut microbiota diversity and structure of tilapia. Therefore, a 0.2 g/kg dose is suggested to supplement the diet of Nile tilapia to improve liver health and modulate the gut microbiota profile of the fish positively. Overall, these findings provide a shred of beneficial evidence of the application of B. velezensis T23 as a green feed additive to improve the productivity and profitability of tilapia farming. |
| format | Article |
| id | doaj-art-103f8cecf64c4fa48903412c3fd3351d |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-103f8cecf64c4fa48903412c3fd3351d2025-02-06T05:12:02ZengElsevierAquaculture Reports2352-51342025-03-0140102523Dietary Solid-state-fermentation product of Bacillus velezensis T23 alleviate hepatic steatosis, oxidative stress, gut barrier damage, and microbiota dysbiosis in juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus)Qiang Hao0Rolf Erik Olsen1Einar Ringø2Qianwen Ding3Tsegay Teame4Yuanyuan Yao5Chao Ran6Yalin Yang7Zhen Zhang8Zhigang Zhou9China-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Norway-China Joint Lab on Fish Gut Microbiota, Department of Biology, Norwegian University of Science and Technology, Trondheim 7491, NorwayNorway-China Joint Lab on Fish Gut Microbiota, Department of Biology, Norwegian University of Science and Technology, Trondheim 7491, NorwayFaculty of Biosciences, Fisheries, and Economics, Norwegian College of Fisheries Science, UiT The Arctic University of Norway, Tromsø N-9037, NorwayKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaChina-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Tigray Agricultural Research Institute, Mekelle, Tigray, EthiopiaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Faculty of Land and Food Systems, The University of British Columbia, Vancouver, Canada; Corresponding author at: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China.Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding author.The application of commensal probiotic bacteria is one of the preferable green feed additives for antibiotic substitution in aquaculture, while the application methods and detailed action mechanisms of commensal bacteria isolated from aquatic animals are not consistent with normal mammalian-derived probiotics. The purpose of this study was to evaluate the effects of solid-state fermentation product of autochthonous Bacillus velezensis T23 on improving hepatic steatosis, liver antioxidation capacity, gut injury and gut microbiota profile of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). The T23 probiotic was added to the basal diet at a level of 0.00, 0.05, 0.1, 0.2 and 0.3 g/kg to develop five experimental diets, respectively. After 4 weeks of the feeding trial, the results showed that T23 supplementation notably reduced the hepatocytes vacuolization and the content of liver TAGs by downregulated the mRNA expression of the lipogenesis gene (fas), while upregulated the mRNA expression of lipid degradation genes (cpt1 and pparα) (p < 0.05). Compared with the control, the 0.3T23 group showed significantly upregulated expression levels of anti-inflammatory cytokine genes (tgf-β and il-10) in the liver of the fish (p < 0.05). Besides, the activities of antioxidase SOD and CAT were significantly upregulated in T23 treatment groups, compared with the control (p < 0.05). Fish groups fed with 0.2 g/kg and 0.3 g/kg T23 supplemented diet also exhibited significantly lower intestinal injury and intestinal inflammation via upregulating the expression of tight junction protein gene claudin, anti-inflammatory factor gene il-10 (p < 0.05). However, markedly downregulation of the mRNA level of pro-inflammatory cytokine il-1β (p < 0.05) was observed in 0.05 and 0.3T23 groups, compared with the control. 16S rRNA sequencing analysis data on gut microbiota indicated that supplementation of T23 at lower levels (0.05, 0.1 and 0.2) resulted in a remarkable elevation of the abundance of phylum Firmicutes and reduced the abundance of Proteobacteria, and the ratio of (Firmicutes +Bacteroidota +Fusobacteria) /Proteobacteria. On the contrary, feeding with 0.3T23 negatively affects the gut microbiota diversity and structure of tilapia. Therefore, a 0.2 g/kg dose is suggested to supplement the diet of Nile tilapia to improve liver health and modulate the gut microbiota profile of the fish positively. Overall, these findings provide a shred of beneficial evidence of the application of B. velezensis T23 as a green feed additive to improve the productivity and profitability of tilapia farming.http://www.sciencedirect.com/science/article/pii/S2352513424006112Bacillus velezensisHepatic steatosisGut healthGut microbiota |
| spellingShingle | Qiang Hao Rolf Erik Olsen Einar Ringø Qianwen Ding Tsegay Teame Yuanyuan Yao Chao Ran Yalin Yang Zhen Zhang Zhigang Zhou Dietary Solid-state-fermentation product of Bacillus velezensis T23 alleviate hepatic steatosis, oxidative stress, gut barrier damage, and microbiota dysbiosis in juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus) Aquaculture Reports Bacillus velezensis Hepatic steatosis Gut health Gut microbiota |
| title | Dietary Solid-state-fermentation product of Bacillus velezensis T23 alleviate hepatic steatosis, oxidative stress, gut barrier damage, and microbiota dysbiosis in juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus) |
| title_full | Dietary Solid-state-fermentation product of Bacillus velezensis T23 alleviate hepatic steatosis, oxidative stress, gut barrier damage, and microbiota dysbiosis in juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus) |
| title_fullStr | Dietary Solid-state-fermentation product of Bacillus velezensis T23 alleviate hepatic steatosis, oxidative stress, gut barrier damage, and microbiota dysbiosis in juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus) |
| title_full_unstemmed | Dietary Solid-state-fermentation product of Bacillus velezensis T23 alleviate hepatic steatosis, oxidative stress, gut barrier damage, and microbiota dysbiosis in juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus) |
| title_short | Dietary Solid-state-fermentation product of Bacillus velezensis T23 alleviate hepatic steatosis, oxidative stress, gut barrier damage, and microbiota dysbiosis in juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus) |
| title_sort | dietary solid state fermentation product of bacillus velezensis t23 alleviate hepatic steatosis oxidative stress gut barrier damage and microbiota dysbiosis in juvenile genetically improved farmed tilapia gift oreochromis niloticus |
| topic | Bacillus velezensis Hepatic steatosis Gut health Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006112 |
| work_keys_str_mv | AT qianghao dietarysolidstatefermentationproductofbacillusvelezensist23alleviatehepaticsteatosisoxidativestressgutbarrierdamageandmicrobiotadysbiosisinjuvenilegeneticallyimprovedfarmedtilapiagiftoreochromisniloticus AT rolferikolsen dietarysolidstatefermentationproductofbacillusvelezensist23alleviatehepaticsteatosisoxidativestressgutbarrierdamageandmicrobiotadysbiosisinjuvenilegeneticallyimprovedfarmedtilapiagiftoreochromisniloticus AT einarringø dietarysolidstatefermentationproductofbacillusvelezensist23alleviatehepaticsteatosisoxidativestressgutbarrierdamageandmicrobiotadysbiosisinjuvenilegeneticallyimprovedfarmedtilapiagiftoreochromisniloticus AT qianwending dietarysolidstatefermentationproductofbacillusvelezensist23alleviatehepaticsteatosisoxidativestressgutbarrierdamageandmicrobiotadysbiosisinjuvenilegeneticallyimprovedfarmedtilapiagiftoreochromisniloticus AT tsegayteame dietarysolidstatefermentationproductofbacillusvelezensist23alleviatehepaticsteatosisoxidativestressgutbarrierdamageandmicrobiotadysbiosisinjuvenilegeneticallyimprovedfarmedtilapiagiftoreochromisniloticus AT yuanyuanyao dietarysolidstatefermentationproductofbacillusvelezensist23alleviatehepaticsteatosisoxidativestressgutbarrierdamageandmicrobiotadysbiosisinjuvenilegeneticallyimprovedfarmedtilapiagiftoreochromisniloticus AT chaoran dietarysolidstatefermentationproductofbacillusvelezensist23alleviatehepaticsteatosisoxidativestressgutbarrierdamageandmicrobiotadysbiosisinjuvenilegeneticallyimprovedfarmedtilapiagiftoreochromisniloticus AT yalinyang dietarysolidstatefermentationproductofbacillusvelezensist23alleviatehepaticsteatosisoxidativestressgutbarrierdamageandmicrobiotadysbiosisinjuvenilegeneticallyimprovedfarmedtilapiagiftoreochromisniloticus AT zhenzhang dietarysolidstatefermentationproductofbacillusvelezensist23alleviatehepaticsteatosisoxidativestressgutbarrierdamageandmicrobiotadysbiosisinjuvenilegeneticallyimprovedfarmedtilapiagiftoreochromisniloticus AT zhigangzhou dietarysolidstatefermentationproductofbacillusvelezensist23alleviatehepaticsteatosisoxidativestressgutbarrierdamageandmicrobiotadysbiosisinjuvenilegeneticallyimprovedfarmedtilapiagiftoreochromisniloticus |