Structure-based insights into fluorogenic RNA aptamers
Fluorogenic RNA aptamers are in vitro-selected RNA molecules capable of binding to specific fluorophores, significantly increasing their intrinsic fluorescence. Over the past decade, the color palette of fluorescent RNA aptamers has greatly...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
China Science Publishing & Media Ltd.
2024-08-01
|
| Series: | Acta Biochimica et Biophysica Sinica |
| Subjects: | |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2024142 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832590306544975872 |
|---|---|
| author | Song Qianqian Tai Xiaoqing Ren Qianyu Ren Aiming |
| author_facet | Song Qianqian Tai Xiaoqing Ren Qianyu Ren Aiming |
| author_sort | Song Qianqian |
| collection | DOAJ |
| description | Fluorogenic RNA aptamers are in vitro-selected RNA molecules capable of binding to specific fluorophores, significantly increasing their intrinsic fluorescence. Over the past decade, the color palette of fluorescent RNA aptamers has greatly expanded. The emergence and development of these fluorogenic RNA aptamers has introduced a powerful approach for visualizing RNA localization and transport with high spatiotemporal resolution in live cells. To date, a variety of tertiary structures of fluorogenic RNA aptamers have been determined using X-ray crystallography or NMR spectroscopy. Many of these fluorogenic RNA aptamers feature base quadruples or base triples in their fluorophore-binding sites. This review summarizes the structure-based investigations of fluorogenic RNA aptamers, with a focus on their overall folds, ligand-binding pockets and fluorescence activation mechanisms. Additionally, the exploration of how structures guide rational optimization to enhance RNA visualization techniques is discussed. |
| format | Article |
| id | doaj-art-0ff9f014426d46f3aa339fbd5ed51e9e |
| institution | Kabale University |
| issn | 1672-9145 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-0ff9f014426d46f3aa339fbd5ed51e9e2025-01-24T00:56:44ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452024-08-015710811810.3724/abbs.202414220d259ccStructure-based insights into fluorogenic RNA aptamersSong Qianqian0Tai Xiaoqing1Ren Qianyu2Ren Aiming3["Life Sciences Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310058, China"]["Life Sciences Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310058, China"]["Agricultural College, Yangzhou University, Yangzhou 225009, China"]["Life Sciences Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310058, China"]Fluorogenic RNA aptamers are in vitro-selected RNA molecules capable of binding to specific fluorophores, significantly increasing their intrinsic fluorescence. Over the past decade, the color palette of fluorescent RNA aptamers has greatly expanded. The emergence and development of these fluorogenic RNA aptamers has introduced a powerful approach for visualizing RNA localization and transport with high spatiotemporal resolution in live cells. To date, a variety of tertiary structures of fluorogenic RNA aptamers have been determined using X-ray crystallography or NMR spectroscopy. Many of these fluorogenic RNA aptamers feature base quadruples or base triples in their fluorophore-binding sites. This review summarizes the structure-based investigations of fluorogenic RNA aptamers, with a focus on their overall folds, ligand-binding pockets and fluorescence activation mechanisms. Additionally, the exploration of how structures guide rational optimization to enhance RNA visualization techniques is discussed.https://www.sciengine.com/doi/10.3724/abbs.2024142fluorogenic RNA aptamerstertiary structuresbase quadruplesbase triplesfluorescence activation mechanismsG-quadruplex |
| spellingShingle | Song Qianqian Tai Xiaoqing Ren Qianyu Ren Aiming Structure-based insights into fluorogenic RNA aptamers Acta Biochimica et Biophysica Sinica fluorogenic RNA aptamers tertiary structures base quadruples base triples fluorescence activation mechanisms G-quadruplex |
| title | Structure-based insights into fluorogenic RNA aptamers |
| title_full | Structure-based insights into fluorogenic RNA aptamers |
| title_fullStr | Structure-based insights into fluorogenic RNA aptamers |
| title_full_unstemmed | Structure-based insights into fluorogenic RNA aptamers |
| title_short | Structure-based insights into fluorogenic RNA aptamers |
| title_sort | structure based insights into fluorogenic rna aptamers |
| topic | fluorogenic RNA aptamers tertiary structures base quadruples base triples fluorescence activation mechanisms G-quadruplex |
| url | https://www.sciengine.com/doi/10.3724/abbs.2024142 |
| work_keys_str_mv | AT songqianqian structurebasedinsightsintofluorogenicrnaaptamers AT taixiaoqing structurebasedinsightsintofluorogenicrnaaptamers AT renqianyu structurebasedinsightsintofluorogenicrnaaptamers AT renaiming structurebasedinsightsintofluorogenicrnaaptamers |