Pro- and Anti-Inflammatory Cytokines Release in Mice Injected with Crotalus durissus terrificus Venom
The effects of Crotalus durissus terrificus venom (Cdt) were analyzed with respect to the susceptibility and the inflammatory mediators in an experimental model of severe envenomation. BALB/c female mice injected intraperitoneally presented sensibility to Cdt, with changes in specific signs, blood b...
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| Format: | Article |
| Language: | English |
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Wiley
2008-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2008/874962 |
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| author | A. Hernández Cruz S. Garcia-Jimenez R. Zucatelli Mendonça V. L. Petricevich |
| author_facet | A. Hernández Cruz S. Garcia-Jimenez R. Zucatelli Mendonça V. L. Petricevich |
| author_sort | A. Hernández Cruz |
| collection | DOAJ |
| description | The effects of Crotalus durissus terrificus venom (Cdt) were analyzed with respect to the susceptibility and the inflammatory mediators in an experimental model of severe envenomation. BALB/c female mice injected intraperitoneally presented sensibility to Cdt, with changes in specific signs, blood biochemical and inflammatory mediators. The venom induced reduction of glucose and urea levels and an increment of creatinine levels in serum from mice. Significant differences were observed in the time-course of mediator levels in sera from mice injected with Cdt. The maximum levels of IL-6, NO, IL-5, TNF, IL-4 and IL-10 were observed 15 min, 30 min, 1, 2 and 4 hours post-injection, respectively. No difference was observed for levels of IFN-γ. Taken together, these data indicate that the envenomation by Cdt is regulated both pro- and anti-inflammatory cytokine responses at time-dependent manner. In serum from mice injected with Cdt at the two first hours revealed of pro-inflammatory dominance. However, with an increment of time an increase of anti-inflammatory cytokines was observed and the balance toward to anti-inflammatory dominance. In conclusion, the observation that Cdt affects the production of pro- and anti-inflammatory cytokines provides further evidence for the role played by Cdt in modulating pro/anti-inflammatory cytokine balance. |
| format | Article |
| id | doaj-art-0f55f1e7811d4818a5cc48ca54b490e9 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-0f55f1e7811d4818a5cc48ca54b490e92025-02-03T07:25:18ZengWileyMediators of Inflammation0962-93511466-18612008-01-01200810.1155/2008/874962874962Pro- and Anti-Inflammatory Cytokines Release in Mice Injected with Crotalus durissus terrificus VenomA. Hernández Cruz0S. Garcia-Jimenez1R. Zucatelli Mendonça2V. L. Petricevich3Laboratorio de Toxicologia, Facultad de Medicina, Universidad Autónoma del Estado de Morelos (UAEM), Avenida Universidad 1001, Cuernavaca, Morelos 62210, MexicoFacultad de Farmacia, Universidad Autónoma del Estado de Morelos (UAEM), Avenida Universidad 1001, Cuernavaca, Morelos 62210, MexicoLaboratorio de Imunologia Viral, Instituto Butantan, Anevida Vital Brasil 1500, Butantã 05503000 São Paulo, SP, BrazilLaboratorio de Toxicologia, Facultad de Medicina, Universidad Autónoma del Estado de Morelos (UAEM), Avenida Universidad 1001, Cuernavaca, Morelos 62210, MexicoThe effects of Crotalus durissus terrificus venom (Cdt) were analyzed with respect to the susceptibility and the inflammatory mediators in an experimental model of severe envenomation. BALB/c female mice injected intraperitoneally presented sensibility to Cdt, with changes in specific signs, blood biochemical and inflammatory mediators. The venom induced reduction of glucose and urea levels and an increment of creatinine levels in serum from mice. Significant differences were observed in the time-course of mediator levels in sera from mice injected with Cdt. The maximum levels of IL-6, NO, IL-5, TNF, IL-4 and IL-10 were observed 15 min, 30 min, 1, 2 and 4 hours post-injection, respectively. No difference was observed for levels of IFN-γ. Taken together, these data indicate that the envenomation by Cdt is regulated both pro- and anti-inflammatory cytokine responses at time-dependent manner. In serum from mice injected with Cdt at the two first hours revealed of pro-inflammatory dominance. However, with an increment of time an increase of anti-inflammatory cytokines was observed and the balance toward to anti-inflammatory dominance. In conclusion, the observation that Cdt affects the production of pro- and anti-inflammatory cytokines provides further evidence for the role played by Cdt in modulating pro/anti-inflammatory cytokine balance.http://dx.doi.org/10.1155/2008/874962 |
| spellingShingle | A. Hernández Cruz S. Garcia-Jimenez R. Zucatelli Mendonça V. L. Petricevich Pro- and Anti-Inflammatory Cytokines Release in Mice Injected with Crotalus durissus terrificus Venom Mediators of Inflammation |
| title | Pro- and Anti-Inflammatory Cytokines Release in Mice Injected with Crotalus durissus terrificus Venom |
| title_full | Pro- and Anti-Inflammatory Cytokines Release in Mice Injected with Crotalus durissus terrificus Venom |
| title_fullStr | Pro- and Anti-Inflammatory Cytokines Release in Mice Injected with Crotalus durissus terrificus Venom |
| title_full_unstemmed | Pro- and Anti-Inflammatory Cytokines Release in Mice Injected with Crotalus durissus terrificus Venom |
| title_short | Pro- and Anti-Inflammatory Cytokines Release in Mice Injected with Crotalus durissus terrificus Venom |
| title_sort | pro and anti inflammatory cytokines release in mice injected with crotalus durissus terrificus venom |
| url | http://dx.doi.org/10.1155/2008/874962 |
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