Stacking and Analysis of Melamine in Milk Products with Acetonitrile-Salt Stacking Technique in Capillary Electrophoresis
Melamine was measured in real milk products with capillary electrophoresis (CE) based on acetonitrile-salt stacking (ASS) method. Real milk samples were deproteinized with acetonitrile at a final concentration of 60% (v/v) and then injected hydrodynamically at 50 mBar for 40.0 s. The optimized buffe...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/212697 |
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| author | Yu Kong Chong Wei Zhanwu Hou Zilong Wang Jiaqiang Yuan Jiang Yu Yongxi Zhao Yuhai Tang Meili Gao |
| author_facet | Yu Kong Chong Wei Zhanwu Hou Zilong Wang Jiaqiang Yuan Jiang Yu Yongxi Zhao Yuhai Tang Meili Gao |
| author_sort | Yu Kong |
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| description | Melamine was measured in real milk products with capillary electrophoresis (CE) based on acetonitrile-salt stacking (ASS) method. Real milk samples were deproteinized with acetonitrile at a final concentration of 60% (v/v) and then injected hydrodynamically at 50 mBar for 40.0 s. The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates. Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L. Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved. The proposed method was suitable for routine assay of MEL in real milk samples that was subjected to a simple treatment step. |
| format | Article |
| id | doaj-art-0ecb24b41bb34b5e924c8525cea85e2f |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-0ecb24b41bb34b5e924c8525cea85e2f2025-02-03T01:11:24ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/212697212697Stacking and Analysis of Melamine in Milk Products with Acetonitrile-Salt Stacking Technique in Capillary ElectrophoresisYu Kong0Chong Wei1Zhanwu Hou2Zilong Wang3Jiaqiang Yuan4Jiang Yu5Yongxi Zhao6Yuhai Tang7Meili Gao8Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaDepartment of Criminal and Inspectoral, Public Security Bureau of Han Zhong City, Shaanxi, ChinaInstitute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaInstitute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaInstitute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaInstitute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaInstitute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaInstitute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaInstitute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaMelamine was measured in real milk products with capillary electrophoresis (CE) based on acetonitrile-salt stacking (ASS) method. Real milk samples were deproteinized with acetonitrile at a final concentration of 60% (v/v) and then injected hydrodynamically at 50 mBar for 40.0 s. The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates. Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L. Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved. The proposed method was suitable for routine assay of MEL in real milk samples that was subjected to a simple treatment step.http://dx.doi.org/10.1155/2014/212697 |
| spellingShingle | Yu Kong Chong Wei Zhanwu Hou Zilong Wang Jiaqiang Yuan Jiang Yu Yongxi Zhao Yuhai Tang Meili Gao Stacking and Analysis of Melamine in Milk Products with Acetonitrile-Salt Stacking Technique in Capillary Electrophoresis Journal of Analytical Methods in Chemistry |
| title | Stacking and Analysis of Melamine in Milk Products with Acetonitrile-Salt Stacking Technique in Capillary Electrophoresis |
| title_full | Stacking and Analysis of Melamine in Milk Products with Acetonitrile-Salt Stacking Technique in Capillary Electrophoresis |
| title_fullStr | Stacking and Analysis of Melamine in Milk Products with Acetonitrile-Salt Stacking Technique in Capillary Electrophoresis |
| title_full_unstemmed | Stacking and Analysis of Melamine in Milk Products with Acetonitrile-Salt Stacking Technique in Capillary Electrophoresis |
| title_short | Stacking and Analysis of Melamine in Milk Products with Acetonitrile-Salt Stacking Technique in Capillary Electrophoresis |
| title_sort | stacking and analysis of melamine in milk products with acetonitrile salt stacking technique in capillary electrophoresis |
| url | http://dx.doi.org/10.1155/2014/212697 |
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