De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer <i>Azohydromonas lata</i> Strain H1 Genome and Genomic Analysis of PHB Production Machinery
Polyhydroxybutyrate (PHB) is a biodegradable natural polymer produced by different prokaryotes as a valuable carbon and energy storage compound. Its biosynthesis pathway requires the sole expression of the <i>phaCAB</i> operon, although auxiliary genes play a role in controlling polymer...
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2025-01-01
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| Online Access: | https://www.mdpi.com/2076-2607/13/1/137 |
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| author | Daniele Traversa Carlo Pazzani Pietro D’Addabbo Lucia Trisolini Matteo Chiara Marta Oliva Angelo Marzella Camilla Mandorino Carla Calia Guglielmina Chimienti Caterina Manzari Graziano Pesole Maria Scrascia |
| author_facet | Daniele Traversa Carlo Pazzani Pietro D’Addabbo Lucia Trisolini Matteo Chiara Marta Oliva Angelo Marzella Camilla Mandorino Carla Calia Guglielmina Chimienti Caterina Manzari Graziano Pesole Maria Scrascia |
| author_sort | Daniele Traversa |
| collection | DOAJ |
| description | Polyhydroxybutyrate (PHB) is a biodegradable natural polymer produced by different prokaryotes as a valuable carbon and energy storage compound. Its biosynthesis pathway requires the sole expression of the <i>phaCAB</i> operon, although auxiliary genes play a role in controlling polymer accumulation, degradation, granule formation and stabilization. Due to its biodegradability, PHB is currently regarded as a promising alternative to synthetic plastics for industrial/biotechnological applications. <i>Azohydromonas lata</i> strain H1 has been reported to accumulate PHB by using simple, inexpensive carbon sources. Here, we present the first de novo genome assembly of the <i>A. lata</i> strain H1. The genome assembly is over 7.7 Mb in size, including a circular megaplasmid of approximately 456 Kbp. In addition to the <i>phaCAB</i> operon, single genes ascribable to PhaC and PhaA functions and auxiliary genes were also detected. A comparative genomic analysis of the available genomes of the genus <i>Azohydromonas</i> revealed the presence of <i>phaCAB</i> and auxiliary genes in all <i>Azohydromonas</i> species investigated, suggesting that the PHB production is a common feature of the genus. Based on sequence identity, we also suggest <i>A. australica</i> as the closest species to which the <i>phaCAB</i> operon of the strain H1, reported in 1998, is similar. |
| format | Article |
| id | doaj-art-0eb6e0d3feb94a1a8809e756b92ceef9 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-0eb6e0d3feb94a1a8809e756b92ceef92025-01-24T13:42:47ZengMDPI AGMicroorganisms2076-26072025-01-0113113710.3390/microorganisms13010137De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer <i>Azohydromonas lata</i> Strain H1 Genome and Genomic Analysis of PHB Production MachineryDaniele Traversa0Carlo Pazzani1Pietro D’Addabbo2Lucia Trisolini3Matteo Chiara4Marta Oliva5Angelo Marzella6Camilla Mandorino7Carla Calia8Guglielmina Chimienti9Caterina Manzari10Graziano Pesole11Maria Scrascia12Department of Biosciences, University of Milan, 20133 Milan, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, ItalyDepartment of Biosciences, University of Milan, 20133 Milan, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, ItalyInstitute of Biomembranes, Bioenergetics and Molecular Biotechnology, Consiglio Nazionale delle Ricerche, 70126 Bari, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, ItalyDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, ItalyPolyhydroxybutyrate (PHB) is a biodegradable natural polymer produced by different prokaryotes as a valuable carbon and energy storage compound. Its biosynthesis pathway requires the sole expression of the <i>phaCAB</i> operon, although auxiliary genes play a role in controlling polymer accumulation, degradation, granule formation and stabilization. Due to its biodegradability, PHB is currently regarded as a promising alternative to synthetic plastics for industrial/biotechnological applications. <i>Azohydromonas lata</i> strain H1 has been reported to accumulate PHB by using simple, inexpensive carbon sources. Here, we present the first de novo genome assembly of the <i>A. lata</i> strain H1. The genome assembly is over 7.7 Mb in size, including a circular megaplasmid of approximately 456 Kbp. In addition to the <i>phaCAB</i> operon, single genes ascribable to PhaC and PhaA functions and auxiliary genes were also detected. A comparative genomic analysis of the available genomes of the genus <i>Azohydromonas</i> revealed the presence of <i>phaCAB</i> and auxiliary genes in all <i>Azohydromonas</i> species investigated, suggesting that the PHB production is a common feature of the genus. Based on sequence identity, we also suggest <i>A. australica</i> as the closest species to which the <i>phaCAB</i> operon of the strain H1, reported in 1998, is similar.https://www.mdpi.com/2076-2607/13/1/137bioplasticbacteria biopolymersbioeconomypolyhydroxyalkanoatesPHAbiodegradable |
| spellingShingle | Daniele Traversa Carlo Pazzani Pietro D’Addabbo Lucia Trisolini Matteo Chiara Marta Oliva Angelo Marzella Camilla Mandorino Carla Calia Guglielmina Chimienti Caterina Manzari Graziano Pesole Maria Scrascia De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer <i>Azohydromonas lata</i> Strain H1 Genome and Genomic Analysis of PHB Production Machinery Microorganisms bioplastic bacteria biopolymers bioeconomy polyhydroxyalkanoates PHA biodegradable |
| title | De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer <i>Azohydromonas lata</i> Strain H1 Genome and Genomic Analysis of PHB Production Machinery |
| title_full | De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer <i>Azohydromonas lata</i> Strain H1 Genome and Genomic Analysis of PHB Production Machinery |
| title_fullStr | De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer <i>Azohydromonas lata</i> Strain H1 Genome and Genomic Analysis of PHB Production Machinery |
| title_full_unstemmed | De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer <i>Azohydromonas lata</i> Strain H1 Genome and Genomic Analysis of PHB Production Machinery |
| title_short | De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer <i>Azohydromonas lata</i> Strain H1 Genome and Genomic Analysis of PHB Production Machinery |
| title_sort | de novo assembly of the polyhydroxybutyrate phb producer i azohydromonas lata i strain h1 genome and genomic analysis of phb production machinery |
| topic | bioplastic bacteria biopolymers bioeconomy polyhydroxyalkanoates PHA biodegradable |
| url | https://www.mdpi.com/2076-2607/13/1/137 |
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