Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway
Inflammation accounts for the process of type II diabetes mellitus (T2DM), the specific mechanism of which is still to be elucidated yet. Nitric oxide (NO), a critical inflammation regulator, the role of which is the inflammation of T2DM, is rarely reported. Therefore, our study is aimed at explorin...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2020/8889612 |
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| author | Hua Guo Qinglan Zhang Haipo Yuan Lin Zhou Fang-fang Li Sheng-Ming Wang Gang Shi Maojuan Wang |
| author_facet | Hua Guo Qinglan Zhang Haipo Yuan Lin Zhou Fang-fang Li Sheng-Ming Wang Gang Shi Maojuan Wang |
| author_sort | Hua Guo |
| collection | DOAJ |
| description | Inflammation accounts for the process of type II diabetes mellitus (T2DM), the specific mechanism of which is still to be elucidated yet. Nitric oxide (NO), a critical inflammation regulator, the role of which is the inflammation of T2DM, is rarely reported. Therefore, our study is aimed at exploring the effect of NO on the inflammation in T2DM and the corresponding mechanism. We analyzed the NO levels in plasma samples from T2DM patients and paired healthy adults by Nitric Oxide Analyzer then measured the expression of inflammatory cytokines (C-reactive protein, heptoglobin, IL-1β, TNF-α, IL-6) in insulin-induced HepG2 cells treated with NO donor or NO scavenger, and the PPARγ, eNOS, C-reactive protein, heptoglobin, IL-1β, TNF-α, and IL-6 levels were detected by RT-PCR and western blot in insulin-induced HepG2 cells transfected with si-PPARγ. The results showed that excess NO increased the inflammation marker levels in T2DM, which is activated by the PPARγ/eNOS pathway. These findings will strengthen the understanding of NO in T2DM and provide a new target for the treatment of T2DM. |
| format | Article |
| id | doaj-art-0e67c136b4f54aea90e1e05a44079a40 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-0e67c136b4f54aea90e1e05a44079a402025-02-03T00:58:52ZengWileyPPAR Research1687-47571687-47652020-01-01202010.1155/2020/88896128889612Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling PathwayHua Guo0Qinglan Zhang1Haipo Yuan2Lin Zhou3Fang-fang Li4Sheng-Ming Wang5Gang Shi6Maojuan Wang7Department of Clinical Laboratory, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Endocrinology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, ChinaDepartment of Endocrinology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Ophthalmology, Huai’an Second People's Hospital, The Affiliated Huai’an Hospital of Xuzhou Medical University, Huai’an, Jiangsu Province, ChinaDepartment of Ophthalmology, Huai’an Second People's Hospital, The Affiliated Huai’an Hospital of Xuzhou Medical University, Huai’an, Jiangsu Province, ChinaDepartment of Stomatology, Huai’an Second People’s Hospital, The Affiliated Huai’an Hospital of Xuzhou Medical University, Huai’an, Jiangsu Province, ChinaDepartment of Pharmacy Services, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Outpatient, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaInflammation accounts for the process of type II diabetes mellitus (T2DM), the specific mechanism of which is still to be elucidated yet. Nitric oxide (NO), a critical inflammation regulator, the role of which is the inflammation of T2DM, is rarely reported. Therefore, our study is aimed at exploring the effect of NO on the inflammation in T2DM and the corresponding mechanism. We analyzed the NO levels in plasma samples from T2DM patients and paired healthy adults by Nitric Oxide Analyzer then measured the expression of inflammatory cytokines (C-reactive protein, heptoglobin, IL-1β, TNF-α, IL-6) in insulin-induced HepG2 cells treated with NO donor or NO scavenger, and the PPARγ, eNOS, C-reactive protein, heptoglobin, IL-1β, TNF-α, and IL-6 levels were detected by RT-PCR and western blot in insulin-induced HepG2 cells transfected with si-PPARγ. The results showed that excess NO increased the inflammation marker levels in T2DM, which is activated by the PPARγ/eNOS pathway. These findings will strengthen the understanding of NO in T2DM and provide a new target for the treatment of T2DM.http://dx.doi.org/10.1155/2020/8889612 |
| spellingShingle | Hua Guo Qinglan Zhang Haipo Yuan Lin Zhou Fang-fang Li Sheng-Ming Wang Gang Shi Maojuan Wang Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway PPAR Research |
| title | Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway |
| title_full | Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway |
| title_fullStr | Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway |
| title_full_unstemmed | Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway |
| title_short | Nitric Oxide Mediates Inflammation in Type II Diabetes Mellitus through the PPARγ/eNOS Signaling Pathway |
| title_sort | nitric oxide mediates inflammation in type ii diabetes mellitus through the pparγ enos signaling pathway |
| url | http://dx.doi.org/10.1155/2020/8889612 |
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