Regulation of TXB2 and PGE2 production by TGF-β1 in in vitro silica dust-exposed rat alveolar macrophage
We investigated the effect of transforming factor factor-β1 (TGF-β1) on thromboxane B2 (TXB2) and prostaglandin E2 (PGE2) production in in vitro silica dust-exposed rat alveolar macrophages (AM). In the presence of 5 μg of anti-TGF-β1 antibodies, TXB2 production decreased, but PGE2 production increa...
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| Format: | Article |
| Language: | English |
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Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935195000664 |
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| _version_ | 1832562996628422656 |
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| author | Urszula Orlinska Douglas C. Kuhn |
| author_facet | Urszula Orlinska Douglas C. Kuhn |
| author_sort | Urszula Orlinska |
| collection | DOAJ |
| description | We investigated the effect of transforming factor factor-β1 (TGF-β1) on thromboxane B2 (TXB2) and prostaglandin E2 (PGE2) production in in vitro silica dust-exposed rat alveolar macrophages (AM). In the presence of 5 μg of anti-TGF-β1 antibodies, TXB2 production decreased, but PGE2 production increased. Addition of 2 ng of TGF-β1 to the culture medium potentiated TXB2 production, but PGE2 production apparently did not change. At 50 ng of TGF-β1, TXB2 production decreased, and PGE2 production varied. Our data suggest that in rat AM: (1) both endogenous and exogenous TGF-β1 regulate TXB2 production; and (2) in the absence of endogenous TGF-β1 the liberation of PGE2 increases; however, exogenous TGF-β1 does not have a regulatory effect on PGE2. |
| format | Article |
| id | doaj-art-0e2e8b5081aa4b1bb5676b3101b81be1 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-0e2e8b5081aa4b1bb5676b3101b81be12025-02-03T01:21:08ZengWileyMediators of Inflammation0962-93511466-18611995-01-014641341610.1155/S0962935195000664Regulation of TXB2 and PGE2 production by TGF-β1 in in vitro silica dust-exposed rat alveolar macrophageUrszula Orlinska0Douglas C. Kuhn1Department of Pathology, Hershey Medical Center, The Pennsylvania State University, P.O. Box 850, Hershey, PA 17033, USADepartment of Pathology, Hershey Medical Center, The Pennsylvania State University, P.O. Box 850, Hershey, PA 17033, USAWe investigated the effect of transforming factor factor-β1 (TGF-β1) on thromboxane B2 (TXB2) and prostaglandin E2 (PGE2) production in in vitro silica dust-exposed rat alveolar macrophages (AM). In the presence of 5 μg of anti-TGF-β1 antibodies, TXB2 production decreased, but PGE2 production increased. Addition of 2 ng of TGF-β1 to the culture medium potentiated TXB2 production, but PGE2 production apparently did not change. At 50 ng of TGF-β1, TXB2 production decreased, and PGE2 production varied. Our data suggest that in rat AM: (1) both endogenous and exogenous TGF-β1 regulate TXB2 production; and (2) in the absence of endogenous TGF-β1 the liberation of PGE2 increases; however, exogenous TGF-β1 does not have a regulatory effect on PGE2.http://dx.doi.org/10.1155/S0962935195000664 |
| spellingShingle | Urszula Orlinska Douglas C. Kuhn Regulation of TXB2 and PGE2 production by TGF-β1 in in vitro silica dust-exposed rat alveolar macrophage Mediators of Inflammation |
| title | Regulation of TXB2 and PGE2 production by TGF-β1 in in vitro silica dust-exposed rat alveolar macrophage |
| title_full | Regulation of TXB2 and PGE2 production by TGF-β1 in in vitro silica dust-exposed rat alveolar macrophage |
| title_fullStr | Regulation of TXB2 and PGE2 production by TGF-β1 in in vitro silica dust-exposed rat alveolar macrophage |
| title_full_unstemmed | Regulation of TXB2 and PGE2 production by TGF-β1 in in vitro silica dust-exposed rat alveolar macrophage |
| title_short | Regulation of TXB2 and PGE2 production by TGF-β1 in in vitro silica dust-exposed rat alveolar macrophage |
| title_sort | regulation of txb2 and pge2 production by tgf β1 in in vitro silica dust exposed rat alveolar macrophage |
| url | http://dx.doi.org/10.1155/S0962935195000664 |
| work_keys_str_mv | AT urszulaorlinska regulationoftxb2andpge2productionbytgfb1ininvitrosilicadustexposedratalveolarmacrophage AT douglasckuhn regulationoftxb2andpge2productionbytgfb1ininvitrosilicadustexposedratalveolarmacrophage |