Effect of Streblus asper Leaf Extract on Scopolamine-Induced Memory Deficits in Zebrafish: The Model of Alzheimer’s Disease
Streblus asper (SA) is well known as a folk medicinal plant in Asian countries. The effect of SA extract on preventing memory impairment in zebrafish induced by scopolamine was investigated. Male zebrafish, Danio rerio, were divided into 6 groups including the control, scopolamine 200 μM (SCO), scop...
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| Format: | Article |
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Wiley
2021-01-01
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| Series: | Advances in Pharmacological and Pharmaceutical Sciences |
| Online Access: | http://dx.doi.org/10.1155/2021/6666726 |
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| author | Kanathip Singsai Natthanicha Ladpala Natthan Dangja Thanyaret Boonchuen Niracha Jaikhamfu Pirinyapat Fakthong |
| author_facet | Kanathip Singsai Natthanicha Ladpala Natthan Dangja Thanyaret Boonchuen Niracha Jaikhamfu Pirinyapat Fakthong |
| author_sort | Kanathip Singsai |
| collection | DOAJ |
| description | Streblus asper (SA) is well known as a folk medicinal plant in Asian countries. The effect of SA extract on preventing memory impairment in zebrafish induced by scopolamine was investigated. Male zebrafish, Danio rerio, were divided into 6 groups including the control, scopolamine 200 μM (SCO), scopolamine plus rivastigmine 1.5 mg/kg (RV + SCO), and scopolamine plus SA extract at doses of 200, 400, and 800 mg/kg (SA200 + SCO, S400 + SCO, and SA800 + SCO), respectively. Spatial memory was evaluated by the colour-biased appetite conditioning T-maze test, while fear memory was measured by the inhibitory avoidance test. In the spatial memory test, results showed that the RV + SCO group had the best time spent ratio in the T-maze, followed by SA800 + SCO, SA400 + SCO, SA200 + SCO, control, and SCO group, respectively, but with no statistical significance. For the fear memory test, zebrafish that received SA at doses of 200, 400, and 800 mg/kg had significantly increased latency time as 21.75 ± 4.59, 23.75 ± 13.01, and 18.20 ± 18.84 min, respectively, when compared to the SCO group (9.80 ± 10.45 min). These results suggested that SA extract attenuated memory impairment in an inhibitory avoidance test related to fear memory. Our findings can be useful for further research to develop SA extract as a health product to ameliorate the symptoms of Alzheimer’s disease. |
| format | Article |
| id | doaj-art-0e28a7c9e488424ba88cbd1994d4ff10 |
| institution | Kabale University |
| issn | 2633-4690 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological and Pharmaceutical Sciences |
| spelling | doaj-art-0e28a7c9e488424ba88cbd1994d4ff102025-02-03T01:28:28ZengWileyAdvances in Pharmacological and Pharmaceutical Sciences2633-46902021-01-01202110.1155/2021/66667266666726Effect of Streblus asper Leaf Extract on Scopolamine-Induced Memory Deficits in Zebrafish: The Model of Alzheimer’s DiseaseKanathip Singsai0Natthanicha Ladpala1Natthan Dangja2Thanyaret Boonchuen3Niracha Jaikhamfu4Pirinyapat Fakthong5Department of Pharmaceutical Care, School of Pharmaceutical Sciences, University of Phayao, Phayao 56000, ThailandDepartment of Pharmaceutical Care, School of Pharmaceutical Sciences, University of Phayao, Phayao 56000, ThailandDepartment of Pharmaceutical Care, School of Pharmaceutical Sciences, University of Phayao, Phayao 56000, ThailandDepartment of Pharmaceutical Care, School of Pharmaceutical Sciences, University of Phayao, Phayao 56000, ThailandDepartment of Pharmaceutical Care, School of Pharmaceutical Sciences, University of Phayao, Phayao 56000, ThailandDepartment of Pharmaceutical Care, School of Pharmaceutical Sciences, University of Phayao, Phayao 56000, ThailandStreblus asper (SA) is well known as a folk medicinal plant in Asian countries. The effect of SA extract on preventing memory impairment in zebrafish induced by scopolamine was investigated. Male zebrafish, Danio rerio, were divided into 6 groups including the control, scopolamine 200 μM (SCO), scopolamine plus rivastigmine 1.5 mg/kg (RV + SCO), and scopolamine plus SA extract at doses of 200, 400, and 800 mg/kg (SA200 + SCO, S400 + SCO, and SA800 + SCO), respectively. Spatial memory was evaluated by the colour-biased appetite conditioning T-maze test, while fear memory was measured by the inhibitory avoidance test. In the spatial memory test, results showed that the RV + SCO group had the best time spent ratio in the T-maze, followed by SA800 + SCO, SA400 + SCO, SA200 + SCO, control, and SCO group, respectively, but with no statistical significance. For the fear memory test, zebrafish that received SA at doses of 200, 400, and 800 mg/kg had significantly increased latency time as 21.75 ± 4.59, 23.75 ± 13.01, and 18.20 ± 18.84 min, respectively, when compared to the SCO group (9.80 ± 10.45 min). These results suggested that SA extract attenuated memory impairment in an inhibitory avoidance test related to fear memory. Our findings can be useful for further research to develop SA extract as a health product to ameliorate the symptoms of Alzheimer’s disease.http://dx.doi.org/10.1155/2021/6666726 |
| spellingShingle | Kanathip Singsai Natthanicha Ladpala Natthan Dangja Thanyaret Boonchuen Niracha Jaikhamfu Pirinyapat Fakthong Effect of Streblus asper Leaf Extract on Scopolamine-Induced Memory Deficits in Zebrafish: The Model of Alzheimer’s Disease Advances in Pharmacological and Pharmaceutical Sciences |
| title | Effect of Streblus asper Leaf Extract on Scopolamine-Induced Memory Deficits in Zebrafish: The Model of Alzheimer’s Disease |
| title_full | Effect of Streblus asper Leaf Extract on Scopolamine-Induced Memory Deficits in Zebrafish: The Model of Alzheimer’s Disease |
| title_fullStr | Effect of Streblus asper Leaf Extract on Scopolamine-Induced Memory Deficits in Zebrafish: The Model of Alzheimer’s Disease |
| title_full_unstemmed | Effect of Streblus asper Leaf Extract on Scopolamine-Induced Memory Deficits in Zebrafish: The Model of Alzheimer’s Disease |
| title_short | Effect of Streblus asper Leaf Extract on Scopolamine-Induced Memory Deficits in Zebrafish: The Model of Alzheimer’s Disease |
| title_sort | effect of streblus asper leaf extract on scopolamine induced memory deficits in zebrafish the model of alzheimer s disease |
| url | http://dx.doi.org/10.1155/2021/6666726 |
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