Effect of Gamma Irradiation on Structural and Biological Properties of a PLGA-PEG-Hydroxyapatite Composite
Gamma irradiation is able to affect various structural and biological properties of biomaterials In this study, a composite of Hap/PLGA-PEG and their ingredients were submitted to gamma irradiation doses of 25 and 50 KGy. Various properties such as molecular weight (GPC), thermal behavior (DSC), wet...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/420616 |
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| author | Sima Shahabi Farhood Najafi Abbas Majdabadi Tabassom Hooshmand Masoumeh Haghbin Nazarpak Batool Karimi Seyyed Mostafa Fatemi |
| author_facet | Sima Shahabi Farhood Najafi Abbas Majdabadi Tabassom Hooshmand Masoumeh Haghbin Nazarpak Batool Karimi Seyyed Mostafa Fatemi |
| author_sort | Sima Shahabi |
| collection | DOAJ |
| description | Gamma irradiation is able to affect various structural and biological properties of biomaterials In this study, a composite of Hap/PLGA-PEG and their ingredients were submitted to gamma irradiation doses of 25 and 50 KGy. Various properties such as molecular weight (GPC), thermal behavior (DSC), wettability (contact angle), cell viability (MTT assay), and alkaline phosphatase activity were studied for the composites and each of their ingredients. The results showed a decrease in molecular weight of copolymer with no change in the glass transition and melting temperatures after gamma irradiation. In general gamma irradiation can increase the activation energy ΔH of the composites and their ingredients. While gamma irradiation had no effect on the wettability of copolymer samples, there was a significant decrease in contact angle of hydroxyapatite and composites with increase in gamma irradiation dose. This study showed an increase in biocompatibility of hydroxyapatite with gamma irradiation with no significant effect on cell viability in copolymer and composite samples. In spite of the fact that no change occurred in alkaline phosphatase activity of composite samples, results indicated a decrease in alkaline phosphatase activity in irradiated hydroxyapatites. These effects on the properties of PLGA-PEG-hydroxyapatite can enhance the composite application as a biomaterial. |
| format | Article |
| id | doaj-art-0d98b7b14a15439885a1210dbe45d1c9 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-0d98b7b14a15439885a1210dbe45d1c92025-02-03T06:08:12ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/420616420616Effect of Gamma Irradiation on Structural and Biological Properties of a PLGA-PEG-Hydroxyapatite CompositeSima Shahabi0Farhood Najafi1Abbas Majdabadi2Tabassom Hooshmand3Masoumeh Haghbin Nazarpak4Batool Karimi5Seyyed Mostafa Fatemi6Department of Dental Biomaterials, School of Dentistry, Tehran University of Medical Sciences, End of North Kargar Street, Tehran, IranDepartment of Resin and Additives, Institute for Color Science and Technology, 55 Vafamanesh Street, HosseinabadSquare, P.O. Box 16765-654, Tehran, IranLaser Research Center of Dentistry, School of Dentistry, Tehran University of Medical Sciences, End of North Kargar Street, Tehran, IranDepartment of Dental Biomaterials, School of Dentistry, Tehran University of Medical Sciences, End of North Kargar Street, Tehran, IranNew Technologies Research Center (NTRC), Amirkabir University of Technology, 424 Hafez Avenue, Tehran, IranIranian Center for Medical Laser Research, ACECR Tehran Medical Sciences Branch, Tehran, IranDepartment of Dental Biomaterials, School of Dentistry, Tehran University of Medical Sciences, End of North Kargar Street, Tehran, IranGamma irradiation is able to affect various structural and biological properties of biomaterials In this study, a composite of Hap/PLGA-PEG and their ingredients were submitted to gamma irradiation doses of 25 and 50 KGy. Various properties such as molecular weight (GPC), thermal behavior (DSC), wettability (contact angle), cell viability (MTT assay), and alkaline phosphatase activity were studied for the composites and each of their ingredients. The results showed a decrease in molecular weight of copolymer with no change in the glass transition and melting temperatures after gamma irradiation. In general gamma irradiation can increase the activation energy ΔH of the composites and their ingredients. While gamma irradiation had no effect on the wettability of copolymer samples, there was a significant decrease in contact angle of hydroxyapatite and composites with increase in gamma irradiation dose. This study showed an increase in biocompatibility of hydroxyapatite with gamma irradiation with no significant effect on cell viability in copolymer and composite samples. In spite of the fact that no change occurred in alkaline phosphatase activity of composite samples, results indicated a decrease in alkaline phosphatase activity in irradiated hydroxyapatites. These effects on the properties of PLGA-PEG-hydroxyapatite can enhance the composite application as a biomaterial.http://dx.doi.org/10.1155/2014/420616 |
| spellingShingle | Sima Shahabi Farhood Najafi Abbas Majdabadi Tabassom Hooshmand Masoumeh Haghbin Nazarpak Batool Karimi Seyyed Mostafa Fatemi Effect of Gamma Irradiation on Structural and Biological Properties of a PLGA-PEG-Hydroxyapatite Composite The Scientific World Journal |
| title | Effect of Gamma Irradiation on Structural and Biological Properties of a PLGA-PEG-Hydroxyapatite Composite |
| title_full | Effect of Gamma Irradiation on Structural and Biological Properties of a PLGA-PEG-Hydroxyapatite Composite |
| title_fullStr | Effect of Gamma Irradiation on Structural and Biological Properties of a PLGA-PEG-Hydroxyapatite Composite |
| title_full_unstemmed | Effect of Gamma Irradiation on Structural and Biological Properties of a PLGA-PEG-Hydroxyapatite Composite |
| title_short | Effect of Gamma Irradiation on Structural and Biological Properties of a PLGA-PEG-Hydroxyapatite Composite |
| title_sort | effect of gamma irradiation on structural and biological properties of a plga peg hydroxyapatite composite |
| url | http://dx.doi.org/10.1155/2014/420616 |
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