Detection of Leishmania donovani DNA within Field-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) in Three Cutaneous Leishmaniasis Endemic Foci of Kurunegala District, Sri Lanka
Leishmaniasis is a parasitic infection transmitted through the bite of female phlebotomine sand flies. Microscopy is the gold standard to detect parasites within the sand flies and for vector incrimination. However, molecular-based detection has become more popular nowadays in the identification of...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2021/6650388 |
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| author | Tharaka Wijerathna Nayana Gunathilaka Kithsiri Gunawardena Yoshito Fujii Deepa Gunasekara |
| author_facet | Tharaka Wijerathna Nayana Gunathilaka Kithsiri Gunawardena Yoshito Fujii Deepa Gunasekara |
| author_sort | Tharaka Wijerathna |
| collection | DOAJ |
| description | Leishmaniasis is a parasitic infection transmitted through the bite of female phlebotomine sand flies. Microscopy is the gold standard to detect parasites within the sand flies and for vector incrimination. However, molecular-based detection has become more popular nowadays in the identification of Leishmania parasites since it provides detection and species identification simultaneously with no need of laborious procedures. The entomological surveys were conducted monthly from May to October 2017 using standard entomological techniques. Field-caught sand flies were identified to the species level followed by DNA extraction. The polymerase chain reaction (PCR) was performed using species-specific primers to detect Leishmania donovani parasites. A total of 1,662 sand flies were encountered from the entomological surveys, and the majority of them were Phlebotomus argentipes (n = 1517; 91.27%), while others were Sergentomyia punjabiensis (n = 140; 8.72%). Leishmania donovani parasite DNA was detected only from P. argentipes (2.3%; n = 2). The detection of Leishmania DNA in P. argentipes suggests the possible role of this species as a vector for leishmaniasis in Sri Lanka. |
| format | Article |
| id | doaj-art-0d0818925cac48dba51f9823832543d3 |
| institution | Kabale University |
| issn | 1687-9686 1687-9694 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-0d0818925cac48dba51f9823832543d32025-02-03T01:20:32ZengWileyJournal of Tropical Medicine1687-96861687-96942021-01-01202110.1155/2021/66503886650388Detection of Leishmania donovani DNA within Field-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) in Three Cutaneous Leishmaniasis Endemic Foci of Kurunegala District, Sri LankaTharaka Wijerathna0Nayana Gunathilaka1Kithsiri Gunawardena2Yoshito Fujii3Deepa Gunasekara4Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri LankaDepartment of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri LankaDepartment of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri LankaVisiting Scholar, Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri LankaDepartment of Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Kelaniya, Ragama, Sri LankaLeishmaniasis is a parasitic infection transmitted through the bite of female phlebotomine sand flies. Microscopy is the gold standard to detect parasites within the sand flies and for vector incrimination. However, molecular-based detection has become more popular nowadays in the identification of Leishmania parasites since it provides detection and species identification simultaneously with no need of laborious procedures. The entomological surveys were conducted monthly from May to October 2017 using standard entomological techniques. Field-caught sand flies were identified to the species level followed by DNA extraction. The polymerase chain reaction (PCR) was performed using species-specific primers to detect Leishmania donovani parasites. A total of 1,662 sand flies were encountered from the entomological surveys, and the majority of them were Phlebotomus argentipes (n = 1517; 91.27%), while others were Sergentomyia punjabiensis (n = 140; 8.72%). Leishmania donovani parasite DNA was detected only from P. argentipes (2.3%; n = 2). The detection of Leishmania DNA in P. argentipes suggests the possible role of this species as a vector for leishmaniasis in Sri Lanka.http://dx.doi.org/10.1155/2021/6650388 |
| spellingShingle | Tharaka Wijerathna Nayana Gunathilaka Kithsiri Gunawardena Yoshito Fujii Deepa Gunasekara Detection of Leishmania donovani DNA within Field-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) in Three Cutaneous Leishmaniasis Endemic Foci of Kurunegala District, Sri Lanka Journal of Tropical Medicine |
| title | Detection of Leishmania donovani DNA within Field-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) in Three Cutaneous Leishmaniasis Endemic Foci of Kurunegala District, Sri Lanka |
| title_full | Detection of Leishmania donovani DNA within Field-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) in Three Cutaneous Leishmaniasis Endemic Foci of Kurunegala District, Sri Lanka |
| title_fullStr | Detection of Leishmania donovani DNA within Field-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) in Three Cutaneous Leishmaniasis Endemic Foci of Kurunegala District, Sri Lanka |
| title_full_unstemmed | Detection of Leishmania donovani DNA within Field-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) in Three Cutaneous Leishmaniasis Endemic Foci of Kurunegala District, Sri Lanka |
| title_short | Detection of Leishmania donovani DNA within Field-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) in Three Cutaneous Leishmaniasis Endemic Foci of Kurunegala District, Sri Lanka |
| title_sort | detection of leishmania donovani dna within field caught phlebotomine sand flies diptera psychodidae in three cutaneous leishmaniasis endemic foci of kurunegala district sri lanka |
| url | http://dx.doi.org/10.1155/2021/6650388 |
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