Laboratory validation of a simplified DNA extraction protocol followed by a portable qPCR detection of M. tuberculosis DNA suitable for point of care settings.

Laboratory validation of a simplified DNA extraction protocol followed by a portable qPCR detection of M. tuberculosis DNA suitable for point of care settings.

Tuberculosis, caused by Mycobacterium tuberculosis, is a treatable and curable disease, and yet remains one of the leading causes of death worldwide. Diagnosis is essential to reducing the number of cases and starting treatment, but costly tests and equipments that require complex infrastructure ham...

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Main Authors: Tainá Dos Santos Soares, Graziele Lima Bello, Ianca Moraes Dos Santos Petry, Maria Rita Castilho Nicola, Larissa Vitoria da Silva, Regina Bones Barcellos, Joana Morez Silvestri, Maria Lucia Rossetti, Alexandre Dias Tavares Costa
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2024-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0302345
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https://doi.org/10.1371/journal.pone.0302345

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