Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis
We studied the bone mesenchymal stem cells (bMSCs) and gene profiles regulated by Er-Xian Decoction (EXD), a traditional Chinese herbal formula widely used for postmenopausal osteoporosis treatment. Six-month-old female Imprinting Control Region mice that underwent ovariectomy were treated with EXD....
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/4079210 |
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| author | Shufen Liu Jianhua Huang Jing Wang Yongjian Zhao Sheng Lu Yongjun Wang Qin Bian |
| author_facet | Shufen Liu Jianhua Huang Jing Wang Yongjian Zhao Sheng Lu Yongjun Wang Qin Bian |
| author_sort | Shufen Liu |
| collection | DOAJ |
| description | We studied the bone mesenchymal stem cells (bMSCs) and gene profiles regulated by Er-Xian Decoction (EXD), a traditional Chinese herbal formula widely used for postmenopausal osteoporosis treatment. Six-month-old female Imprinting Control Region mice that underwent ovariectomy were treated with EXD. After 3 months, bone mass was evaluated by μCT and histological and immunohistochemical detection. The self-renewal and differentiation capacities of bMSCs were evaluated by colony-forming unit-fibroblastic, colony-forming unit-adipocyte, and alkaline phosphatase staining. In addition, the expression of 26991 genes of bMSCs ex vivo at 2 weeks after EXD-treatment or of bMSCs in vitro after exposure to conditioned serum from EXD-treated rats was measured and analyzed using NimbleGen Gene Expression Profiling and Cluster and pathway analysis. EXD treatment increased bone mass, elevating osteocalcin protein levels in vivo and facilitating the self-renewal and osteoblastic differentiation of bMSCs ex vivo. EXD rescued several gene expressions that were dysregulated by OVX. These genes overlapped and their functions were involved in ten pathways between ex vivo and in vitro experiments. EXD exerts an osteogenic effect on bMSCs in OVX induced osteoporotic mice. Our results contribute to further study of its molecular mechanism and traditional use in the treatment of postmenopausal osteoporosis. |
| format | Article |
| id | doaj-art-0c9cfdd35dbf45bbb74802349d51e5b7 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-0c9cfdd35dbf45bbb74802349d51e5b72025-02-03T01:11:16ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/40792104079210Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile AnalysisShufen Liu0Jianhua Huang1Jing Wang2Yongjian Zhao3Sheng Lu4Yongjun Wang5Qin Bian6Department of Orthopaedics & Traumatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, No. 725 South Wan-ping Road, Shanghai 200032, ChinaInstitute of Integrated Traditional Chinese Medicine & Western Medicine, Huashan Hospital, Fudan University, No. 12 Middle Wulumuqi Road, Shanghai 200040, ChinaDepartment of Orthopaedics & Traumatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, No. 725 South Wan-ping Road, Shanghai 200032, ChinaDepartment of Orthopaedics & Traumatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, No. 725 South Wan-ping Road, Shanghai 200032, ChinaDepartment of Orthopaedics & Traumatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, No. 725 South Wan-ping Road, Shanghai 200032, ChinaDepartment of Orthopaedics & Traumatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, No. 725 South Wan-ping Road, Shanghai 200032, ChinaDepartment of Orthopaedics & Traumatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, No. 725 South Wan-ping Road, Shanghai 200032, ChinaWe studied the bone mesenchymal stem cells (bMSCs) and gene profiles regulated by Er-Xian Decoction (EXD), a traditional Chinese herbal formula widely used for postmenopausal osteoporosis treatment. Six-month-old female Imprinting Control Region mice that underwent ovariectomy were treated with EXD. After 3 months, bone mass was evaluated by μCT and histological and immunohistochemical detection. The self-renewal and differentiation capacities of bMSCs were evaluated by colony-forming unit-fibroblastic, colony-forming unit-adipocyte, and alkaline phosphatase staining. In addition, the expression of 26991 genes of bMSCs ex vivo at 2 weeks after EXD-treatment or of bMSCs in vitro after exposure to conditioned serum from EXD-treated rats was measured and analyzed using NimbleGen Gene Expression Profiling and Cluster and pathway analysis. EXD treatment increased bone mass, elevating osteocalcin protein levels in vivo and facilitating the self-renewal and osteoblastic differentiation of bMSCs ex vivo. EXD rescued several gene expressions that were dysregulated by OVX. These genes overlapped and their functions were involved in ten pathways between ex vivo and in vitro experiments. EXD exerts an osteogenic effect on bMSCs in OVX induced osteoporotic mice. Our results contribute to further study of its molecular mechanism and traditional use in the treatment of postmenopausal osteoporosis.http://dx.doi.org/10.1155/2016/4079210 |
| spellingShingle | Shufen Liu Jianhua Huang Jing Wang Yongjian Zhao Sheng Lu Yongjun Wang Qin Bian Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis Stem Cells International |
| title | Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis |
| title_full | Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis |
| title_fullStr | Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis |
| title_full_unstemmed | Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis |
| title_short | Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis |
| title_sort | er xian decoction stimulates osteoblastic differentiation of bone mesenchymal stem cells in ovariectomized mice and its gene profile analysis |
| url | http://dx.doi.org/10.1155/2016/4079210 |
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