Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of Folkloric Lannea coromandelica Using In Vitro and In Vivo Assays
The aim of this study was to determine the impending antioxidant properties of different extracts of crude methanolic extract (CME) of leaves of Lannea coromandelica (L. coromandelica) and its two ethyl acetate (EAF) and aqueous (AqF) subfractions by employing various established in vitro systems...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2015/203679 |
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| author | Tekeshwar Kumar Vishal Jain |
| author_facet | Tekeshwar Kumar Vishal Jain |
| author_sort | Tekeshwar Kumar |
| collection | DOAJ |
| description | The aim of this study was to determine the impending antioxidant properties of different extracts of crude methanolic extract (CME) of leaves of Lannea coromandelica (L. coromandelica) and its two ethyl acetate (EAF) and aqueous (AqF) subfractions by employing various established in vitro systems and estimation of total phenolic and flavonoid content. The results showed that extract and fractions possessed strong antioxidant activity in vitro and among them, EAF had the strongest antioxidant activity. EAF was confirmed for its highest phenolic content, total flavonoid contents, and total antioxidant capacity. The EAF was found to show remarkable scavenging activity on 2,2-diphenylpicrylhydrazyl (DPPH) (EC50 63.9 ± 0.64 µg/mL), superoxide radical (EC50 8.2 ± 0.12 mg/mL), and Fe2+ chelating activity (EC50 6.2 ± 0.09 mg/mL). Based on our in vitro results, EAF was investigated for in vivo antioxidant assay. Intragastric administration of the EAF can significantly increase levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels, and decrease malondialdehyde (MDA) content in the liver and kidney of CCl4-intoxicated rats. These new evidences show that L. coromandelica bared antioxidant activity. |
| format | Article |
| id | doaj-art-0c76209620134913ae62d2f8621c77d3 |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-0c76209620134913ae62d2f8621c77d32025-02-03T01:11:48ZengWileyScientifica2090-908X2015-01-01201510.1155/2015/203679203679Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of Folkloric Lannea coromandelica Using In Vitro and In Vivo AssaysTekeshwar Kumar0Vishal Jain1University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh 492 010, IndiaUniversity Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh 492 010, IndiaThe aim of this study was to determine the impending antioxidant properties of different extracts of crude methanolic extract (CME) of leaves of Lannea coromandelica (L. coromandelica) and its two ethyl acetate (EAF) and aqueous (AqF) subfractions by employing various established in vitro systems and estimation of total phenolic and flavonoid content. The results showed that extract and fractions possessed strong antioxidant activity in vitro and among them, EAF had the strongest antioxidant activity. EAF was confirmed for its highest phenolic content, total flavonoid contents, and total antioxidant capacity. The EAF was found to show remarkable scavenging activity on 2,2-diphenylpicrylhydrazyl (DPPH) (EC50 63.9 ± 0.64 µg/mL), superoxide radical (EC50 8.2 ± 0.12 mg/mL), and Fe2+ chelating activity (EC50 6.2 ± 0.09 mg/mL). Based on our in vitro results, EAF was investigated for in vivo antioxidant assay. Intragastric administration of the EAF can significantly increase levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels, and decrease malondialdehyde (MDA) content in the liver and kidney of CCl4-intoxicated rats. These new evidences show that L. coromandelica bared antioxidant activity.http://dx.doi.org/10.1155/2015/203679 |
| spellingShingle | Tekeshwar Kumar Vishal Jain Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of Folkloric Lannea coromandelica Using In Vitro and In Vivo Assays Scientifica |
| title | Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of Folkloric Lannea coromandelica Using In Vitro and In Vivo Assays |
| title_full | Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of Folkloric Lannea coromandelica Using In Vitro and In Vivo Assays |
| title_fullStr | Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of Folkloric Lannea coromandelica Using In Vitro and In Vivo Assays |
| title_full_unstemmed | Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of Folkloric Lannea coromandelica Using In Vitro and In Vivo Assays |
| title_short | Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of Folkloric Lannea coromandelica Using In Vitro and In Vivo Assays |
| title_sort | appraisal of total phenol flavonoid contents and antioxidant potential of folkloric lannea coromandelica using in vitro and in vivo assays |
| url | http://dx.doi.org/10.1155/2015/203679 |
| work_keys_str_mv | AT tekeshwarkumar appraisaloftotalphenolflavonoidcontentsandantioxidantpotentialoffolkloriclanneacoromandelicausinginvitroandinvivoassays AT vishaljain appraisaloftotalphenolflavonoidcontentsandantioxidantpotentialoffolkloriclanneacoromandelicausinginvitroandinvivoassays |