Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities
This research the preventive effect of Kudingcha polyphenols (Kp) on colitis based on animal experiments. Experimental mice divided into four groups, including normal group, model group, low-concentration Kp (LKP) group and high-concentration Kp (HKP) group, and they all smeared and given 0.15 mL 1%...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2018-09-01
|
| Series: | Food Science and Human Wellness |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453017302045 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557756484157440 |
|---|---|
| author | Xingyao Long Yanni Pan Xin Zhao |
| author_facet | Xingyao Long Yanni Pan Xin Zhao |
| author_sort | Xingyao Long |
| collection | DOAJ |
| description | This research the preventive effect of Kudingcha polyphenols (Kp) on colitis based on animal experiments. Experimental mice divided into four groups, including normal group, model group, low-concentration Kp (LKP) group and high-concentration Kp (HKP) group, and they all smeared and given 0.15 mL 1% oxazolone solution by lavage to induce BALB/c mice colitis. DAI, colon weight/length ratio, serum levels of cytokines, related antioxidant activities of colon tissues such, and the mRNA expressions. The experimental results show that KP can significantly (p < 0.05) reduce DAI in mice with colitis and inhibit shortening of colon caused by colitis, increase colon weight/length ratio. It can also significantly reduce (p < 0.05) the activities of MPO, NO, MDA in mice colon tissues with colitis, increase the activity of GSH, and significantly increase (p < 0.05) the level of IL-2 and decrease the level of IL-10 in mice serum with colitis. The results of qPCR assay show that Kp can significantly increase (p < 0.05) the expression of Cu/Zn-SOD, Mn-SOD, GSH-Px, CAT, c-Kit, SCF and decrease the expression of IL-8, CXCR2 in mice colon tissues with colitis. Kp has a good preventive effect on colitis, and high concentration of PT has better preventive effect. Keywords: Kudingcha polyphenols, Oxazolone, Antioxidant, Colitis, mRNA expression |
| format | Article |
| id | doaj-art-0c342d8adc644540a74b8d1f158d2876 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-0c342d8adc644540a74b8d1f158d28762025-02-03T02:34:05ZengTsinghua University PressFood Science and Human Wellness2213-45302018-09-0173209214Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacitiesXingyao Long0Yanni Pan1Xin Zhao2Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, PR China; College of Food Science, Southwest University, Chongqing 400715, PR ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, PR China; School of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, PR ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, PR China; School of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, PR China; Corresponding author at: Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, PR China.This research the preventive effect of Kudingcha polyphenols (Kp) on colitis based on animal experiments. Experimental mice divided into four groups, including normal group, model group, low-concentration Kp (LKP) group and high-concentration Kp (HKP) group, and they all smeared and given 0.15 mL 1% oxazolone solution by lavage to induce BALB/c mice colitis. DAI, colon weight/length ratio, serum levels of cytokines, related antioxidant activities of colon tissues such, and the mRNA expressions. The experimental results show that KP can significantly (p < 0.05) reduce DAI in mice with colitis and inhibit shortening of colon caused by colitis, increase colon weight/length ratio. It can also significantly reduce (p < 0.05) the activities of MPO, NO, MDA in mice colon tissues with colitis, increase the activity of GSH, and significantly increase (p < 0.05) the level of IL-2 and decrease the level of IL-10 in mice serum with colitis. The results of qPCR assay show that Kp can significantly increase (p < 0.05) the expression of Cu/Zn-SOD, Mn-SOD, GSH-Px, CAT, c-Kit, SCF and decrease the expression of IL-8, CXCR2 in mice colon tissues with colitis. Kp has a good preventive effect on colitis, and high concentration of PT has better preventive effect. Keywords: Kudingcha polyphenols, Oxazolone, Antioxidant, Colitis, mRNA expressionhttp://www.sciencedirect.com/science/article/pii/S2213453017302045 |
| spellingShingle | Xingyao Long Yanni Pan Xin Zhao Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities Food Science and Human Wellness |
| title | Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities |
| title_full | Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities |
| title_fullStr | Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities |
| title_full_unstemmed | Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities |
| title_short | Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities |
| title_sort | prophylactic effect of kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities |
| url | http://www.sciencedirect.com/science/article/pii/S2213453017302045 |
| work_keys_str_mv | AT xingyaolong prophylacticeffectofkudingchapolyphenolsonoxazoloneinducedcolitisthroughitsantioxidantcapacities AT yannipan prophylacticeffectofkudingchapolyphenolsonoxazoloneinducedcolitisthroughitsantioxidantcapacities AT xinzhao prophylacticeffectofkudingchapolyphenolsonoxazoloneinducedcolitisthroughitsantioxidantcapacities |