Diagnostic Role of Plasma MicroRNA-21 in Stable and Unstable Angina Patients and Association with Aging
The present study explored the clinical value of plasma microRNA-21 as a novel biomarker for early prediction of stable and unstable angina patients and its relationship with aging. A total of 255 participants, 123 patients with chronic stable angina, 82 patients with unstable angina, and 50 healthy...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Cardiology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2020/9093151 |
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| _version_ | 1832561336159043584 |
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| author | Md Sayed Ali Sheikh |
| author_facet | Md Sayed Ali Sheikh |
| author_sort | Md Sayed Ali Sheikh |
| collection | DOAJ |
| description | The present study explored the clinical value of plasma microRNA-21 as a novel biomarker for early prediction of stable and unstable angina patients and its relationship with aging. A total of 255 participants, 123 patients with chronic stable angina, 82 patients with unstable angina, and 50 healthy subjects, were included in our study. Stable coronary and unstable coronary patients were confirmed following AHA/ACC clinical protocols. Total RNA was extracted from plasma by using miRNA-based TRIzol reagent. Plasma miR-21 expression levels were determined by real-time polymerase chain reaction. To evaluate the diagnosis accuracy, the receiver operating characteristic (ROC) curves were used. Plasma microRNA-21 concentration levels were significantly elevated in stable and unstable angina patients as compared with control subjects P<0.001. The area under the ROC curves of circulating microRNA-21 was accurately distinguished in stable angina patients (AUC 0.921) and unstable angina patients (AUC 0.944) from healthy subjects. MicroRNA-21 expression gradually elevated with increasing aging in all the populations. Moreover, the current study also demonstrated that the expression of plasma miR-21 levels was significantly associated with different age groups within healthy subjects and stable and unstable angina patients P<0.001. This research finding suggested that plasma microRNA-21 may be considered as a suitable new biomarker for early detection of stable and unstable angina patients, and it has a strong correlation with aging. |
| format | Article |
| id | doaj-art-0c13e900dabd4dbfa16fc0ca967ec09d |
| institution | Kabale University |
| issn | 2090-8016 2090-0597 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiology Research and Practice |
| spelling | doaj-art-0c13e900dabd4dbfa16fc0ca967ec09d2025-02-03T01:25:17ZengWileyCardiology Research and Practice2090-80162090-05972020-01-01202010.1155/2020/90931519093151Diagnostic Role of Plasma MicroRNA-21 in Stable and Unstable Angina Patients and Association with AgingMd Sayed Ali Sheikh0Department of Internal Medicine, Cardiology, College of Medicine, Jouf University, Sakaka, Aljouf, Saudi ArabiaThe present study explored the clinical value of plasma microRNA-21 as a novel biomarker for early prediction of stable and unstable angina patients and its relationship with aging. A total of 255 participants, 123 patients with chronic stable angina, 82 patients with unstable angina, and 50 healthy subjects, were included in our study. Stable coronary and unstable coronary patients were confirmed following AHA/ACC clinical protocols. Total RNA was extracted from plasma by using miRNA-based TRIzol reagent. Plasma miR-21 expression levels were determined by real-time polymerase chain reaction. To evaluate the diagnosis accuracy, the receiver operating characteristic (ROC) curves were used. Plasma microRNA-21 concentration levels were significantly elevated in stable and unstable angina patients as compared with control subjects P<0.001. The area under the ROC curves of circulating microRNA-21 was accurately distinguished in stable angina patients (AUC 0.921) and unstable angina patients (AUC 0.944) from healthy subjects. MicroRNA-21 expression gradually elevated with increasing aging in all the populations. Moreover, the current study also demonstrated that the expression of plasma miR-21 levels was significantly associated with different age groups within healthy subjects and stable and unstable angina patients P<0.001. This research finding suggested that plasma microRNA-21 may be considered as a suitable new biomarker for early detection of stable and unstable angina patients, and it has a strong correlation with aging.http://dx.doi.org/10.1155/2020/9093151 |
| spellingShingle | Md Sayed Ali Sheikh Diagnostic Role of Plasma MicroRNA-21 in Stable and Unstable Angina Patients and Association with Aging Cardiology Research and Practice |
| title | Diagnostic Role of Plasma MicroRNA-21 in Stable and Unstable Angina Patients and Association with Aging |
| title_full | Diagnostic Role of Plasma MicroRNA-21 in Stable and Unstable Angina Patients and Association with Aging |
| title_fullStr | Diagnostic Role of Plasma MicroRNA-21 in Stable and Unstable Angina Patients and Association with Aging |
| title_full_unstemmed | Diagnostic Role of Plasma MicroRNA-21 in Stable and Unstable Angina Patients and Association with Aging |
| title_short | Diagnostic Role of Plasma MicroRNA-21 in Stable and Unstable Angina Patients and Association with Aging |
| title_sort | diagnostic role of plasma microrna 21 in stable and unstable angina patients and association with aging |
| url | http://dx.doi.org/10.1155/2020/9093151 |
| work_keys_str_mv | AT mdsayedalisheikh diagnosticroleofplasmamicrorna21instableandunstableanginapatientsandassociationwithaging |