IL-4 induces cAMP and cGMP in human monocytic cells
Human monocytes, preincubated with IFN-γ respond to IL-4 by a cGMP increase through activation of an inducible NO synthase. Here, IL-4 was found to induce an accumulation of cGMP (1 – 3 min) and cAMP (20 – 25 min) in unstimulated monocytes. This was impaired with NOS inhibitors, but also with EGTA a...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935195000482 |
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| author | B. Dugas N. Paul-Eugene K. Yamaoka C. Amirand C. Damais J. P. Kolb |
| author_facet | B. Dugas N. Paul-Eugene K. Yamaoka C. Amirand C. Damais J. P. Kolb |
| author_sort | B. Dugas |
| collection | DOAJ |
| description | Human monocytes, preincubated with IFN-γ respond to IL-4 by a cGMP increase through activation of an inducible NO synthase. Here, IL-4 was found to induce an accumulation of cGMP (1 – 3 min) and cAMP (20 – 25 min) in unstimulated monocytes. This was impaired with NOS inhibitors, but also with EGTA and calcium/calmodulin inhibitors. These results suggest that: (1) IL-4 may stimulate different NOS isoforms in resting and IFN-γ activated monocytes, and (2) cAMP accumulation may be partially dependent on the NO pathway. By RT-PCR, a type III constitutive NOS mRNA was detected in U937 monocytic cells. IL-4 also increased the [Ca2+]i in these cells. Different NOS may thus be expressed in monocytic cells depending on their differentiation and the signals they receive. |
| format | Article |
| id | doaj-art-0bff7f74e70e450496a00503ce3bb480 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-0bff7f74e70e450496a00503ce3bb4802025-02-03T05:54:23ZengWileyMediators of Inflammation0962-93511466-18611995-01-014429830510.1155/S0962935195000482IL-4 induces cAMP and cGMP in human monocytic cellsB. Dugas0N. Paul-Eugene1K. Yamaoka2C. Amirand3C. Damais4J. P. Kolb5URA CNRS 625, Hôpital Pitié-Salpêtrière, Paris 75013, FranceINSERM U313, Centre BioMédical des Cordeliers, Paris 75006, FranceINSERM U365, Institut Curie, Paris 75005, FranceURA CNRS 198, Institut Curie, Paris 75005, FranceINSERM U313, Centre BioMédical des Cordeliers, Paris 75006, FranceINSERM U365, Institut Curie, Paris 75005, FranceHuman monocytes, preincubated with IFN-γ respond to IL-4 by a cGMP increase through activation of an inducible NO synthase. Here, IL-4 was found to induce an accumulation of cGMP (1 – 3 min) and cAMP (20 – 25 min) in unstimulated monocytes. This was impaired with NOS inhibitors, but also with EGTA and calcium/calmodulin inhibitors. These results suggest that: (1) IL-4 may stimulate different NOS isoforms in resting and IFN-γ activated monocytes, and (2) cAMP accumulation may be partially dependent on the NO pathway. By RT-PCR, a type III constitutive NOS mRNA was detected in U937 monocytic cells. IL-4 also increased the [Ca2+]i in these cells. Different NOS may thus be expressed in monocytic cells depending on their differentiation and the signals they receive.http://dx.doi.org/10.1155/S0962935195000482 |
| spellingShingle | B. Dugas N. Paul-Eugene K. Yamaoka C. Amirand C. Damais J. P. Kolb IL-4 induces cAMP and cGMP in human monocytic cells Mediators of Inflammation |
| title | IL-4 induces cAMP and cGMP in human monocytic cells |
| title_full | IL-4 induces cAMP and cGMP in human monocytic cells |
| title_fullStr | IL-4 induces cAMP and cGMP in human monocytic cells |
| title_full_unstemmed | IL-4 induces cAMP and cGMP in human monocytic cells |
| title_short | IL-4 induces cAMP and cGMP in human monocytic cells |
| title_sort | il 4 induces camp and cgmp in human monocytic cells |
| url | http://dx.doi.org/10.1155/S0962935195000482 |
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