Steady-state acceptor fluorescence anisotropy imaging under evanescent excitation for visualisation of FRET at the plasma membrane.

Steady-state acceptor fluorescence anisotropy imaging under evanescent excitation for visualisation of FRET at the plasma membrane.

We present a novel imaging system combining total internal reflection fluorescence (TIRF) microscopy with measurement of steady-state acceptor fluorescence anisotropy in order to perform live cell Förster Resonance Energy Transfer (FRET) imaging at the plasma membrane. We compare directly the imagin...

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Bibliographic Details
Main Authors: Viviane Devauges, Daniel R Matthews, Justin Aluko, Jakub Nedbal, James A Levitt, Simon P Poland, Oana Coban, Gregory Weitsman, James Monypenny, Tony Ng, Simon M Ameer-Beg
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0110695
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https://doi.org/10.1371/journal.pone.0110695

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