Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls
Sperm motility is a key factor influencing male fertility and is associated with metabolic and lipid profiles across species. The aim of this study was to investigate the relationship between sperm motility and the seminal plasma lipid profile in Simmental bulls, and to identify key lipids potential...
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2025-01-01
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| author | Zhuo Yang Fang Luo Chenglei Song Zhiyuan Ma Yucheng Tian Yu Fu Hao Zheng Jinzhong Tao |
| author_facet | Zhuo Yang Fang Luo Chenglei Song Zhiyuan Ma Yucheng Tian Yu Fu Hao Zheng Jinzhong Tao |
| author_sort | Zhuo Yang |
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| description | Sperm motility is a key factor influencing male fertility and is associated with metabolic and lipid profiles across species. The aim of this study was to investigate the relationship between sperm motility and the seminal plasma lipid profile in Simmental bulls, and to identify key lipids potentially influencing sperm motility. Semen samples were collected from 26 healthy Simmental bulls with an average age of 4.9 years. Sperm quality was evaluated using computer-assisted sperm analysis (CASA). Based on motility, the samples were divided into two groups: high sperm motility (HSM > 65%) and low sperm motility (LSM < 65%). Compared to the LSM group, the HSM group exhibited significantly higher sperm viability, motility, straight-line velocity, beat-cross frequency, and sperm acrosome integrity, while the sperm malformation rate was lower (<i>p</i> < 0.05). Lipid profiles were determined using LC-MS/MS, and 40 differential lipids were identified by multivariate statistical analysis. Among them, 39 lipids were upregulated in the LSM group compared to the HSM group. They were primarily triglycerides and carnitines, mainly involved in four metabolic pathways related to glycerophospholipid and linoleic acid metabolism. Notably, PC (16:0/20:4; 14:0/18:3), LPC (22:4/0:0; 22:6/0:0), and PE (14:0/18:1; 18:1/20:3) were diagnosed with great accuracy (AUC > 0.7), which means they may serve as potential biomarkers for sperm motility. |
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| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-0aa5df55a140460d85f0b6531d46da472025-01-24T13:18:23ZengMDPI AGAnimals2076-26152025-01-0115227610.3390/ani15020276Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental BullsZhuo Yang0Fang Luo1Chenglei Song2Zhiyuan Ma3Yucheng Tian4Yu Fu5Hao Zheng6Jinzhong Tao7College of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaStation of Domestic Animal Improving and Management of Gansu Province, Wuwei 733000, ChinaNingxia Breeding Cow Biotechnology Co., Ltd., Zhongwei 751800, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaSperm motility is a key factor influencing male fertility and is associated with metabolic and lipid profiles across species. The aim of this study was to investigate the relationship between sperm motility and the seminal plasma lipid profile in Simmental bulls, and to identify key lipids potentially influencing sperm motility. Semen samples were collected from 26 healthy Simmental bulls with an average age of 4.9 years. Sperm quality was evaluated using computer-assisted sperm analysis (CASA). Based on motility, the samples were divided into two groups: high sperm motility (HSM > 65%) and low sperm motility (LSM < 65%). Compared to the LSM group, the HSM group exhibited significantly higher sperm viability, motility, straight-line velocity, beat-cross frequency, and sperm acrosome integrity, while the sperm malformation rate was lower (<i>p</i> < 0.05). Lipid profiles were determined using LC-MS/MS, and 40 differential lipids were identified by multivariate statistical analysis. Among them, 39 lipids were upregulated in the LSM group compared to the HSM group. They were primarily triglycerides and carnitines, mainly involved in four metabolic pathways related to glycerophospholipid and linoleic acid metabolism. Notably, PC (16:0/20:4; 14:0/18:3), LPC (22:4/0:0; 22:6/0:0), and PE (14:0/18:1; 18:1/20:3) were diagnosed with great accuracy (AUC > 0.7), which means they may serve as potential biomarkers for sperm motility.https://www.mdpi.com/2076-2615/15/2/276CASAsperm parametersmetabolic pathwayslipid profilesbiomarker |
| spellingShingle | Zhuo Yang Fang Luo Chenglei Song Zhiyuan Ma Yucheng Tian Yu Fu Hao Zheng Jinzhong Tao Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls Animals CASA sperm parameters metabolic pathways lipid profiles biomarker |
| title | Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls |
| title_full | Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls |
| title_fullStr | Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls |
| title_full_unstemmed | Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls |
| title_short | Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls |
| title_sort | elevated lipid concentrations in seminal plasma can reduce sperm motility in simmental bulls |
| topic | CASA sperm parameters metabolic pathways lipid profiles biomarker |
| url | https://www.mdpi.com/2076-2615/15/2/276 |
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