Treatment of tumour tissue with radio‐frequency hyperthermia (using antibody‐carrying nanoparticles)
Abstract Intelligent inorganic nanoparticles were designed and produced for use in imaging and annihilating tumour cells by radio‐frequency (RF) hyperthermia. Nanoparticles synthesised to provide RF hyperthermia must have magnetite properties. For this purpose, magnetite nanoparticles were first syn...
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| Format: | Article |
| Language: | English |
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Wiley
2021-10-01
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| Series: | IET Nanobiotechnology |
| Online Access: | https://doi.org/10.1049/nbt2.12061 |
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| author | Reza Didarian Ibrahim Vargel |
| author_facet | Reza Didarian Ibrahim Vargel |
| author_sort | Reza Didarian |
| collection | DOAJ |
| description | Abstract Intelligent inorganic nanoparticles were designed and produced for use in imaging and annihilating tumour cells by radio‐frequency (RF) hyperthermia. Nanoparticles synthesised to provide RF hyperthermia must have magnetite properties. For this purpose, magnetite nanoparticles were first synthesised by the coprecipitation method (10–15 NM). These superparamagnetic nanoparticles were then covered with gold ions without losing their magnetic properties. In this step, gold ions are reduced around the magnetite nanoparticles. Surface modification of the gold‐coated magnetic nanoparticles was performed in the next step. A self‐assembled monolayer was created using cysteamine (2‐aminoethanethiol) molecules, which have two different end groups (SH and NH2). These molecules react with the gold surface by SH groups. The NH2 groups give a positive charge to the nanoparticles. After that, a monoclonal antibody (Monoclonal Anti‐N‐CAM Clone NCAM‐OB11) was immobilised by the 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide/N‐hydroxysuccinimide method. Then, the antenna RF system (144.00015 MHz) was created for RF hyperthermia. The antibody‐nanoparticle binding rate and cytotoxicity tests were followed by in vitro and in vivo experiments. As the main result, antibody‐bound gold‐coated magnetic nanoparticles were successfully connected to tumour cells. After RF hyperthermia, the tumour size decreased owing to apoptosis and necrosis of tumour cells. |
| format | Article |
| id | doaj-art-0a7aa150f18e437899818716b23e4c4a |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2021-10-01 |
| publisher | Wiley |
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| series | IET Nanobiotechnology |
| spelling | doaj-art-0a7aa150f18e437899818716b23e4c4a2025-02-03T01:29:38ZengWileyIET Nanobiotechnology1751-87411751-875X2021-10-0115863965310.1049/nbt2.12061Treatment of tumour tissue with radio‐frequency hyperthermia (using antibody‐carrying nanoparticles)Reza Didarian0Ibrahim Vargel1Department of Nanotechnology and Nanomedicine Hacettepe University Beytepe Campus Ankara TurkeyDepartment of Plastic Reconstructive and Aesthetic Surgery Hacettepe University, Sıhhiye Ankara TurkeyAbstract Intelligent inorganic nanoparticles were designed and produced for use in imaging and annihilating tumour cells by radio‐frequency (RF) hyperthermia. Nanoparticles synthesised to provide RF hyperthermia must have magnetite properties. For this purpose, magnetite nanoparticles were first synthesised by the coprecipitation method (10–15 NM). These superparamagnetic nanoparticles were then covered with gold ions without losing their magnetic properties. In this step, gold ions are reduced around the magnetite nanoparticles. Surface modification of the gold‐coated magnetic nanoparticles was performed in the next step. A self‐assembled monolayer was created using cysteamine (2‐aminoethanethiol) molecules, which have two different end groups (SH and NH2). These molecules react with the gold surface by SH groups. The NH2 groups give a positive charge to the nanoparticles. After that, a monoclonal antibody (Monoclonal Anti‐N‐CAM Clone NCAM‐OB11) was immobilised by the 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide/N‐hydroxysuccinimide method. Then, the antenna RF system (144.00015 MHz) was created for RF hyperthermia. The antibody‐nanoparticle binding rate and cytotoxicity tests were followed by in vitro and in vivo experiments. As the main result, antibody‐bound gold‐coated magnetic nanoparticles were successfully connected to tumour cells. After RF hyperthermia, the tumour size decreased owing to apoptosis and necrosis of tumour cells.https://doi.org/10.1049/nbt2.12061 |
| spellingShingle | Reza Didarian Ibrahim Vargel Treatment of tumour tissue with radio‐frequency hyperthermia (using antibody‐carrying nanoparticles) IET Nanobiotechnology |
| title | Treatment of tumour tissue with radio‐frequency hyperthermia (using antibody‐carrying nanoparticles) |
| title_full | Treatment of tumour tissue with radio‐frequency hyperthermia (using antibody‐carrying nanoparticles) |
| title_fullStr | Treatment of tumour tissue with radio‐frequency hyperthermia (using antibody‐carrying nanoparticles) |
| title_full_unstemmed | Treatment of tumour tissue with radio‐frequency hyperthermia (using antibody‐carrying nanoparticles) |
| title_short | Treatment of tumour tissue with radio‐frequency hyperthermia (using antibody‐carrying nanoparticles) |
| title_sort | treatment of tumour tissue with radio frequency hyperthermia using antibody carrying nanoparticles |
| url | https://doi.org/10.1049/nbt2.12061 |
| work_keys_str_mv | AT rezadidarian treatmentoftumourtissuewithradiofrequencyhyperthermiausingantibodycarryingnanoparticles AT ibrahimvargel treatmentoftumourtissuewithradiofrequencyhyperthermiausingantibodycarryingnanoparticles |