Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks

A set of four duplex SYBR Green I PCR (SG-PCR) assay combined with DNA extraction using QIAamp DNA Stool Mini kit was evaluated for the detection of foodborne bacteria from 21 foodborne outbreaks. The causative pathogens were detected in almost all cases in 2 hours or less. The first run was for the...

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Bibliographic Details
Main Authors: Hiroshi Fukushima, Kazunori Katsube, Yoshie Tsunomori, Ryoko Kishi, Junko Atsuta, Yuko Akiba
Format: Article
Language:English
Published: Wiley 2009-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/2009/917623
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