Electrochemical Sandwich Immunoassay for the Ultrasensitive Detection of Human MUC1 Cancer Biomarker
A new electrochemical sandwich immunoassay for the ultrasensitive detection of human MUC1 cancer biomarker using protein G-functionalized magnetic beads (MBs) and graphite-based screen-printed electrodes (SPEs) was developed. Magnetic beads were employed as the platforms for the immobilization and i...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Electrochemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/740265 |
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| _version_ | 1832549697516994560 |
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| author | Zahra Taleat Cecilia Cristea Giovanna Marrazza Robert Săndulescu |
| author_facet | Zahra Taleat Cecilia Cristea Giovanna Marrazza Robert Săndulescu |
| author_sort | Zahra Taleat |
| collection | DOAJ |
| description | A new electrochemical sandwich immunoassay for the ultrasensitive detection of human MUC1 cancer biomarker using protein G-functionalized magnetic beads (MBs) and graphite-based screen-printed electrodes (SPEs) was developed. Magnetic beads were employed as the platforms for the immobilization and immunoreaction process. A pair of primary and secondary antibodies was used to capture the MUC1 protein. After labeling with a third antibody conjugated with horseradish peroxidase (HRP), the resulting conjugate was trapped at the surface of the graphite-based SPEs and MUC1 determination was carried out by differential pulse voltammetry (DPV) at 0.4 V upon H2O2 addition using acetaminophen (APAP) as the redox mediator. A linear relationship was obtained for the detection of human MUC1 over a range of 0–25 ppb with the lowest detection limit of 1.34 ppb when HRP was applied as a label. Preliminary experiments were performed using disposable electrochemical sensors in order to optimize some parameters (i.e., incubation times, concentrations, and blocking agent). |
| format | Article |
| id | doaj-art-08e88018840c4628865d7cc50f26fc84 |
| institution | Kabale University |
| issn | 2090-3529 2090-3537 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Electrochemistry |
| spelling | doaj-art-08e88018840c4628865d7cc50f26fc842025-02-03T06:08:47ZengWileyInternational Journal of Electrochemistry2090-35292090-35372013-01-01201310.1155/2013/740265740265Electrochemical Sandwich Immunoassay for the Ultrasensitive Detection of Human MUC1 Cancer BiomarkerZahra Taleat0Cecilia Cristea1Giovanna Marrazza2Robert Săndulescu3Analytical Chemistry Department, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 4 Pasteur Street, 400021 Cluj-Napoca, RomaniaAnalytical Chemistry Department, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 4 Pasteur Street, 400021 Cluj-Napoca, RomaniaDepartment of Chemistry Ugo Schiff, University of Science Florence, P.zza S.Marco, 4, 50121 Florence, ItalyAnalytical Chemistry Department, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 4 Pasteur Street, 400021 Cluj-Napoca, RomaniaA new electrochemical sandwich immunoassay for the ultrasensitive detection of human MUC1 cancer biomarker using protein G-functionalized magnetic beads (MBs) and graphite-based screen-printed electrodes (SPEs) was developed. Magnetic beads were employed as the platforms for the immobilization and immunoreaction process. A pair of primary and secondary antibodies was used to capture the MUC1 protein. After labeling with a third antibody conjugated with horseradish peroxidase (HRP), the resulting conjugate was trapped at the surface of the graphite-based SPEs and MUC1 determination was carried out by differential pulse voltammetry (DPV) at 0.4 V upon H2O2 addition using acetaminophen (APAP) as the redox mediator. A linear relationship was obtained for the detection of human MUC1 over a range of 0–25 ppb with the lowest detection limit of 1.34 ppb when HRP was applied as a label. Preliminary experiments were performed using disposable electrochemical sensors in order to optimize some parameters (i.e., incubation times, concentrations, and blocking agent).http://dx.doi.org/10.1155/2013/740265 |
| spellingShingle | Zahra Taleat Cecilia Cristea Giovanna Marrazza Robert Săndulescu Electrochemical Sandwich Immunoassay for the Ultrasensitive Detection of Human MUC1 Cancer Biomarker International Journal of Electrochemistry |
| title | Electrochemical Sandwich Immunoassay for the Ultrasensitive Detection of Human MUC1 Cancer Biomarker |
| title_full | Electrochemical Sandwich Immunoassay for the Ultrasensitive Detection of Human MUC1 Cancer Biomarker |
| title_fullStr | Electrochemical Sandwich Immunoassay for the Ultrasensitive Detection of Human MUC1 Cancer Biomarker |
| title_full_unstemmed | Electrochemical Sandwich Immunoassay for the Ultrasensitive Detection of Human MUC1 Cancer Biomarker |
| title_short | Electrochemical Sandwich Immunoassay for the Ultrasensitive Detection of Human MUC1 Cancer Biomarker |
| title_sort | electrochemical sandwich immunoassay for the ultrasensitive detection of human muc1 cancer biomarker |
| url | http://dx.doi.org/10.1155/2013/740265 |
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