用Taqman MGB探针研究中国人群TNF-α基因启动子区单核苷酸多态性

【目的】了解中国人群肿瘤坏死因子α(the tumour necrosis factor α,TNF-α)基因启动子区多态性。【方法】 随机选取20名深圳地区汉族健康体检者,采用两对引物PCR扩增TNF-α基因启动子区(-1389nt~+125nt),对PCR产物进行序列分析,寻找单核苷酸多态性位点(single nueleofide polymorphisms,SNPs)。采用Taqman MGB探针建立了-857nt(C/T)位点的实时定量PCR(thereal-time PCR)分型方法,并对中国汉族、壮族、布依族,水族及苗族群体共l108份样本进行了基因分型。【结果】在启动子区(-13...

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Bibliographic Details
Main Authors: 庾蕾, 庄志雄, 谢富焕, 黄海雄, 叶小明
Format: Article
Language:zho
Published: Editorial Office of Journal of Sun Yat-sen University 2006-01-01
Series:Zhongshan Daxue xuebao. Yixue kexue ban
Subjects:
Online Access:http://xuebaoyx.sysu.edu.cn/zh/article/43584142/
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