Effect of refrigeration, room temperature, and processing time on serum immunofluorescent antibody titers for Sarcocystis neurona
Abstract Background Evaluating antibody titers for Sarcocystis neurona for the diagnosis of equine protozoal myeloencephalitis from serum samples is a common practice. However, ensuring timely and proper refrigeration is not always possible. Objectives To evaluate immunofluorescent antibody (IFA) ti...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Journal of Veterinary Internal Medicine |
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| Online Access: | https://doi.org/10.1111/jvim.17282 |
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| author | Claudia Valderrama‐Martinez Andrea Packham Shichen Zheng Woutrina Smith Magdalena Plancarte Monica Aleman |
| author_facet | Claudia Valderrama‐Martinez Andrea Packham Shichen Zheng Woutrina Smith Magdalena Plancarte Monica Aleman |
| author_sort | Claudia Valderrama‐Martinez |
| collection | DOAJ |
| description | Abstract Background Evaluating antibody titers for Sarcocystis neurona for the diagnosis of equine protozoal myeloencephalitis from serum samples is a common practice. However, ensuring timely and proper refrigeration is not always possible. Objectives To evaluate immunofluorescent antibody (IFA) titers for S. neurona from serum samples stored at room temperature and 4°C. Samples Twenty‐two serum samples. Methods Prospective longitudinal study. Two serum aliquots of 1 mL each were stored at room temperature (20‐23.3°C) and 4°C. The unrefrigerated aliquot was immediately tested for IFA titers. Both aliquots were retested on Days 5 and 10 after collection. A paired t test was used to compare IFA titers at different time points. Results There was no significant difference between IFA titers from baseline with those stored at room temperature at Days 5 (P = .741, 95% CI [−56.83, 78.65]), 10 (P = .677, 95% CI [−50.01, 75.46]), and between 5 and 10 days (P = 0.949, 95% CI [−57.50, 61.14]). There was no significant difference from baseline with those stored at 4°C for Days 5 (P = .964, 95% CI [−81.81, 85.45]), 10 (P = 0.573, 95% CI [−109.4, 62.15]), and between 5 and 10 days (P = .5, 95% CI [−102.6, 51.67]). There was no statistical difference between samples stored at room temperature and 4°C (P = .688, CI [−55.51, 37.33]) on Days 5 and 10 (P = .104, CI [−80.8, 8.07]). Conclusions and Clinical Importance Immunofluorescent antibody test titers for S. neurona are stable for up to 10 days at room temperature and 4°C. |
| format | Article |
| id | doaj-art-088c066a3c264dada8babf00609e6ec8 |
| institution | Kabale University |
| issn | 0891-6640 1939-1676 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj-art-088c066a3c264dada8babf00609e6ec82025-01-27T15:22:41ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762025-01-01391n/an/a10.1111/jvim.17282Effect of refrigeration, room temperature, and processing time on serum immunofluorescent antibody titers for Sarcocystis neuronaClaudia Valderrama‐Martinez0Andrea Packham1Shichen Zheng2Woutrina Smith3Magdalena Plancarte4Monica Aleman5Departamento de Salud Animal, Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional de Colombia Bogotá ColombiaOne Health Institute, School of Veterinary Medicine University of California Davis Davis California USADepartment of Medicine and Epidemiology, School of Veterinary Medicine University of California Davis Davis California USAOne Health Institute, School of Veterinary Medicine University of California Davis Davis California USAOne Health Institute, School of Veterinary Medicine University of California Davis Davis California USADepartment of Medicine and Epidemiology, School of Veterinary Medicine University of California Davis Davis California USAAbstract Background Evaluating antibody titers for Sarcocystis neurona for the diagnosis of equine protozoal myeloencephalitis from serum samples is a common practice. However, ensuring timely and proper refrigeration is not always possible. Objectives To evaluate immunofluorescent antibody (IFA) titers for S. neurona from serum samples stored at room temperature and 4°C. Samples Twenty‐two serum samples. Methods Prospective longitudinal study. Two serum aliquots of 1 mL each were stored at room temperature (20‐23.3°C) and 4°C. The unrefrigerated aliquot was immediately tested for IFA titers. Both aliquots were retested on Days 5 and 10 after collection. A paired t test was used to compare IFA titers at different time points. Results There was no significant difference between IFA titers from baseline with those stored at room temperature at Days 5 (P = .741, 95% CI [−56.83, 78.65]), 10 (P = .677, 95% CI [−50.01, 75.46]), and between 5 and 10 days (P = 0.949, 95% CI [−57.50, 61.14]). There was no significant difference from baseline with those stored at 4°C for Days 5 (P = .964, 95% CI [−81.81, 85.45]), 10 (P = 0.573, 95% CI [−109.4, 62.15]), and between 5 and 10 days (P = .5, 95% CI [−102.6, 51.67]). There was no statistical difference between samples stored at room temperature and 4°C (P = .688, CI [−55.51, 37.33]) on Days 5 and 10 (P = .104, CI [−80.8, 8.07]). Conclusions and Clinical Importance Immunofluorescent antibody test titers for S. neurona are stable for up to 10 days at room temperature and 4°C.https://doi.org/10.1111/jvim.17282ambient temperatureantibodiescoldproteinprotozoastability |
| spellingShingle | Claudia Valderrama‐Martinez Andrea Packham Shichen Zheng Woutrina Smith Magdalena Plancarte Monica Aleman Effect of refrigeration, room temperature, and processing time on serum immunofluorescent antibody titers for Sarcocystis neurona Journal of Veterinary Internal Medicine ambient temperature antibodies cold protein protozoa stability |
| title | Effect of refrigeration, room temperature, and processing time on serum immunofluorescent antibody titers for Sarcocystis neurona |
| title_full | Effect of refrigeration, room temperature, and processing time on serum immunofluorescent antibody titers for Sarcocystis neurona |
| title_fullStr | Effect of refrigeration, room temperature, and processing time on serum immunofluorescent antibody titers for Sarcocystis neurona |
| title_full_unstemmed | Effect of refrigeration, room temperature, and processing time on serum immunofluorescent antibody titers for Sarcocystis neurona |
| title_short | Effect of refrigeration, room temperature, and processing time on serum immunofluorescent antibody titers for Sarcocystis neurona |
| title_sort | effect of refrigeration room temperature and processing time on serum immunofluorescent antibody titers for sarcocystis neurona |
| topic | ambient temperature antibodies cold protein protozoa stability |
| url | https://doi.org/10.1111/jvim.17282 |
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