Nitric oxide production in murine spleen cells: role of interferons and prostaglandin E2 in the generation of cytotoxic activity
The production of nitric oxide (NO) was measured in cultures of spleen cells stimulated by lipopolysaccharide (LPS), IL-2 or LPS + IL-2. We observed that NO synthesis is increased by IFN-γ but inhibited by IFN-α/β. This is not the case when IL-2 is present in the cultures, since interferons play a m...
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| Format: | Article |
| Language: | English |
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Wiley
1996-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935196000117 |
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| author | Dominique Vaillier Richard Daculsi Norbert Gualdel |
| author_facet | Dominique Vaillier Richard Daculsi Norbert Gualdel |
| author_sort | Dominique Vaillier |
| collection | DOAJ |
| description | The production of nitric oxide (NO) was measured in cultures of spleen cells stimulated by lipopolysaccharide (LPS), IL-2 or LPS + IL-2. We observed that NO synthesis is increased by IFN-γ but inhibited by IFN-α/β. This is not the case when IL-2 is present in the cultures, since interferons play a minor role in the regulation of the NO production. When IL-2 and LPS were associated in the cultures, the IFN-α/β role seems more important than that of IFN-γ. PGE2 inhibits NO production in LPS supplemented cultures but has a slight effect in the presence of IL-2 and no effect with IL-2 + LPS. 3-isoButyl-1-methylxanthine (IBMX), an inhibitor of phosphodiesterases, induces a decrease of IFN production. In the presence of H-7, an inhibitor of protein kinase C (PKC), NO production is reduced when the cultures are supplemented by LPS or IL-2 but not when IL-2 and LPS are both added. H-7 also reduced IFN production. In the presence of NG-monomethyl-L-arginine (N-MMA), an inhibitor of NO synthesis, IFN production was increased, with no change in the cytotoxic activity. Hence, interferons regulate NO production by mouse spleen cells and, in return, NO modulates the generation of IFN. |
| format | Article |
| id | doaj-art-06ff7c47b9924016a5dafcc6a65b4a97 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1996-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-06ff7c47b9924016a5dafcc6a65b4a972025-02-03T01:30:46ZengWileyMediators of Inflammation0962-93511466-18611996-01-0151626810.1155/S0962935196000117Nitric oxide production in murine spleen cells: role of interferons and prostaglandin E2 in the generation of cytotoxic activityDominique Vaillier0Richard Daculsi1Norbert Gualdel2URA 1456 CNRS, Université de Bordeaux II, FranceURA 1456 CNRS, Université de Bordeaux II, FranceURA 1456 CNRS, Université de Bordeaux II, FranceThe production of nitric oxide (NO) was measured in cultures of spleen cells stimulated by lipopolysaccharide (LPS), IL-2 or LPS + IL-2. We observed that NO synthesis is increased by IFN-γ but inhibited by IFN-α/β. This is not the case when IL-2 is present in the cultures, since interferons play a minor role in the regulation of the NO production. When IL-2 and LPS were associated in the cultures, the IFN-α/β role seems more important than that of IFN-γ. PGE2 inhibits NO production in LPS supplemented cultures but has a slight effect in the presence of IL-2 and no effect with IL-2 + LPS. 3-isoButyl-1-methylxanthine (IBMX), an inhibitor of phosphodiesterases, induces a decrease of IFN production. In the presence of H-7, an inhibitor of protein kinase C (PKC), NO production is reduced when the cultures are supplemented by LPS or IL-2 but not when IL-2 and LPS are both added. H-7 also reduced IFN production. In the presence of NG-monomethyl-L-arginine (N-MMA), an inhibitor of NO synthesis, IFN production was increased, with no change in the cytotoxic activity. Hence, interferons regulate NO production by mouse spleen cells and, in return, NO modulates the generation of IFN.http://dx.doi.org/10.1155/S0962935196000117 |
| spellingShingle | Dominique Vaillier Richard Daculsi Norbert Gualdel Nitric oxide production in murine spleen cells: role of interferons and prostaglandin E2 in the generation of cytotoxic activity Mediators of Inflammation |
| title | Nitric oxide production in murine spleen cells: role of interferons and prostaglandin E2 in the generation of cytotoxic activity |
| title_full | Nitric oxide production in murine spleen cells: role of interferons and prostaglandin E2 in the generation of cytotoxic activity |
| title_fullStr | Nitric oxide production in murine spleen cells: role of interferons and prostaglandin E2 in the generation of cytotoxic activity |
| title_full_unstemmed | Nitric oxide production in murine spleen cells: role of interferons and prostaglandin E2 in the generation of cytotoxic activity |
| title_short | Nitric oxide production in murine spleen cells: role of interferons and prostaglandin E2 in the generation of cytotoxic activity |
| title_sort | nitric oxide production in murine spleen cells role of interferons and prostaglandin e2 in the generation of cytotoxic activity |
| url | http://dx.doi.org/10.1155/S0962935196000117 |
| work_keys_str_mv | AT dominiquevaillier nitricoxideproductioninmurinespleencellsroleofinterferonsandprostaglandine2inthegenerationofcytotoxicactivity AT richarddaculsi nitricoxideproductioninmurinespleencellsroleofinterferonsandprostaglandine2inthegenerationofcytotoxicactivity AT norbertgualdel nitricoxideproductioninmurinespleencellsroleofinterferonsandprostaglandine2inthegenerationofcytotoxicactivity |