TESTING POTATO COLLECTION SAMPLES FOR THE PRESENCE OF GENES FOR RESISTANCE TO PHYTOPATHOGENS BY MEANS OF DNA MARKERS
The development of phytopathogen-resistant varieties is the most reliable and economic way to reduce potato yield losses. Breeding of such varieties is possible by using genetic sources of resistance. The use of DNA markers for identification of valuable genotypes, including forms with several resis...
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| Format: | Article |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2018-03-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/1358 |
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| author | A. B. Saynakova M. S. Romanova S. N. Krasnikov O. V. Litvinchuk Ya. I. Alekseev A. V. Nikulin E. V. Terentjeva |
| author_facet | A. B. Saynakova M. S. Romanova S. N. Krasnikov O. V. Litvinchuk Ya. I. Alekseev A. V. Nikulin E. V. Terentjeva |
| author_sort | A. B. Saynakova |
| collection | DOAJ |
| description | The development of phytopathogen-resistant varieties is the most reliable and economic way to reduce potato yield losses. Breeding of such varieties is possible by using genetic sources of resistance. The use of DNA markers for identification of valuable genotypes, including forms with several resistance genes, makes it possible to significantly improve breeding efficiency. The development of a multiplex PCR technique and using it to simultaneously test varieties and breeding lines for several genes that control the resistance to viruses and nematodes is a new approach to using DNA markers. This study is aimed at screening samples from the collection of the Narym Department of Breeding and Seed Production of the Siberian Research Institute of Agriculture and Peat (the Branch of the Siberian Federal Agrobiotechnology Research Center, the Russian Academy of Sciences) using the multiplex PCR technique, for genes for resistance to Globodera rostochiensis and Globodera pallidas, potato wart disease, viruses X and Y. 40 samples were tested by means of genetic markers to identify genes for resistance to potato wart disease (Sen1), virus X (Rx), virus Y (Ryadg, Rychc, Rysto), Globodera rostochiensis (H1, Gro1-4) and Globodera pallida (Gpa2), in the genome. The sample included two varieties, three populations produced by self-pollination of the Ideal variety, and 35 individually selected potato hybrids. As a result, we identified marker NL25 (Sen1) in 19 samples; marker PVX (Rx) in 13 samples; marker RYSC3312 (Ryadg) in 10 samples; marker YES3-3A341 (Rysto) in 5 samples; markers TG 689141, 57R450, N195337 (H1) in 12 samples; marker Gro1-4-1602 (Gro1-4) in 6 samples; marker Gpa2-2452 (Gpa2) in 13 samples. In terms of economically valuable traits, sample С-31-15 is noted for high yield and quality indicators. It carries genes for resistance to potato virus X (Rx), Y (Rysto), Globodera rostochiensis (H1, Gro1-4), and Globodera pallida (Gpa2). |
| format | Article |
| id | doaj-art-06d7d65083be471d80d9e2f4c6dbc0b2 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2018-03-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-06d7d65083be471d80d9e2f4c6dbc0b22025-02-01T09:58:05ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592018-03-01221182410.18699/VJ18.326718TESTING POTATO COLLECTION SAMPLES FOR THE PRESENCE OF GENES FOR RESISTANCE TO PHYTOPATHOGENS BY MEANS OF DNA MARKERSA. B. Saynakova0M. S. Romanova1S. N. Krasnikov2O. V. Litvinchuk3Ya. I. Alekseev4A. V. Nikulin5E. V. Terentjeva6Siberian Institute of Agriculture and Peat Research – the Branch of the Siberian Federal Agricultural Biotechnology Science Center RASSiberian Institute of Agriculture and Peat Research – the Branch of the Siberian Federal Agricultural Biotechnology Science Center RASSiberian Institute of Agriculture and Peat Research – the Branch of the Siberian Federal Agricultural Biotechnology Science Center RASSiberian Institute of Agriculture and Peat Research – the Branch of the Siberian Federal Agricultural Biotechnology Science Center RASAll-Russia Research Institute of Agricultural Biotechnology; Limited Liability Company SyntolAll-Russia Research Institute of Agricultural BiotechnologyAll-Russia Research Institute of Agricultural Biotechnology; Limited Liability Company SyntolThe development of phytopathogen-resistant varieties is the most reliable and economic way to reduce potato yield losses. Breeding of such varieties is possible by using genetic sources of resistance. The use of DNA markers for identification of valuable genotypes, including forms with several resistance genes, makes it possible to significantly improve breeding efficiency. The development of a multiplex PCR technique and using it to simultaneously test varieties and breeding lines for several genes that control the resistance to viruses and nematodes is a new approach to using DNA markers. This study is aimed at screening samples from the collection of the Narym Department of Breeding and Seed Production of the Siberian Research Institute of Agriculture and Peat (the Branch of the Siberian Federal Agrobiotechnology Research Center, the Russian Academy of Sciences) using the multiplex PCR technique, for genes for resistance to Globodera rostochiensis and Globodera pallidas, potato wart disease, viruses X and Y. 40 samples were tested by means of genetic markers to identify genes for resistance to potato wart disease (Sen1), virus X (Rx), virus Y (Ryadg, Rychc, Rysto), Globodera rostochiensis (H1, Gro1-4) and Globodera pallida (Gpa2), in the genome. The sample included two varieties, three populations produced by self-pollination of the Ideal variety, and 35 individually selected potato hybrids. As a result, we identified marker NL25 (Sen1) in 19 samples; marker PVX (Rx) in 13 samples; marker RYSC3312 (Ryadg) in 10 samples; marker YES3-3A341 (Rysto) in 5 samples; markers TG 689141, 57R450, N195337 (H1) in 12 samples; marker Gro1-4-1602 (Gro1-4) in 6 samples; marker Gpa2-2452 (Gpa2) in 13 samples. In terms of economically valuable traits, sample С-31-15 is noted for high yield and quality indicators. It carries genes for resistance to potato virus X (Rx), Y (Rysto), Globodera rostochiensis (H1, Gro1-4), and Globodera pallida (Gpa2).https://vavilov.elpub.ru/jour/article/view/1358potatoesbreedingcollection samplesresistance genesdna markers |
| spellingShingle | A. B. Saynakova M. S. Romanova S. N. Krasnikov O. V. Litvinchuk Ya. I. Alekseev A. V. Nikulin E. V. Terentjeva TESTING POTATO COLLECTION SAMPLES FOR THE PRESENCE OF GENES FOR RESISTANCE TO PHYTOPATHOGENS BY MEANS OF DNA MARKERS Вавиловский журнал генетики и селекции potatoes breeding collection samples resistance genes dna markers |
| title | TESTING POTATO COLLECTION SAMPLES FOR THE PRESENCE OF GENES FOR RESISTANCE TO PHYTOPATHOGENS BY MEANS OF DNA MARKERS |
| title_full | TESTING POTATO COLLECTION SAMPLES FOR THE PRESENCE OF GENES FOR RESISTANCE TO PHYTOPATHOGENS BY MEANS OF DNA MARKERS |
| title_fullStr | TESTING POTATO COLLECTION SAMPLES FOR THE PRESENCE OF GENES FOR RESISTANCE TO PHYTOPATHOGENS BY MEANS OF DNA MARKERS |
| title_full_unstemmed | TESTING POTATO COLLECTION SAMPLES FOR THE PRESENCE OF GENES FOR RESISTANCE TO PHYTOPATHOGENS BY MEANS OF DNA MARKERS |
| title_short | TESTING POTATO COLLECTION SAMPLES FOR THE PRESENCE OF GENES FOR RESISTANCE TO PHYTOPATHOGENS BY MEANS OF DNA MARKERS |
| title_sort | testing potato collection samples for the presence of genes for resistance to phytopathogens by means of dna markers |
| topic | potatoes breeding collection samples resistance genes dna markers |
| url | https://vavilov.elpub.ru/jour/article/view/1358 |
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