Borrelia burgdorferi Tolerance-Associated Changes in Gene Expression of Murine Macrophages
Lyme disease, caused by the spirochete Borrelia burgdorferi (Bb), is the most prevalent vector-borne disease in the United States. Macrophages, key cellular players in the innate immune response, exhibit diminished functionality over time during Bb infection, potentially leading to chronic infectio...
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| Format: | Article |
| Language: | English |
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The University of Toledo
2025-02-01
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| Series: | Translation |
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| Online Access: | https://openjournals.utoledo.edu/index.php/translation/article/view/1536 |
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| author | Sophia Chen Ricki Deng Anis Hadi Sara Nandwana William Ryan John B. Presloid R. Mark Wooten |
| author_facet | Sophia Chen Ricki Deng Anis Hadi Sara Nandwana William Ryan John B. Presloid R. Mark Wooten |
| author_sort | Sophia Chen |
| collection | DOAJ |
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Lyme disease, caused by the spirochete Borrelia burgdorferi (Bb), is the most prevalent vector-borne disease in the United States. Macrophages, key cellular players in the innate immune response, exhibit diminished functionality over time during Bb infection, potentially leading to chronic infection. This study explores the transcriptional changes associated with macrophages that develop tolerance to Bb. Using RNA sequencing of murine bone marrow-derived macrophages exposed to Bb, we identified differentially expressed genes and dysregulated pathways between productively stimulated and tolerized macrophages. Key findings revealed significant downregulation of type-I interferon signaling and associated immune responses, suggesting mechanisms of immune tolerance. Additionally, connectivity analysis identified potential drug candidates for repurposing to enhance macrophage activity. Our results underscore the complexity of macrophage responses to Bb and provide a foundation for future research to develop targeted therapies aimed at modulating immune responses and improving treatment outcomes for Lyme disease patients. Ultimately, these findings offer new insights into the pathogenesis and potential treatment strategies for Lyme disease.
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| format | Article |
| id | doaj-art-060d33c83e3242bfb43bc65b2336572d |
| institution | Kabale University |
| issn | 2469-6706 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | The University of Toledo |
| record_format | Article |
| series | Translation |
| spelling | doaj-art-060d33c83e3242bfb43bc65b2336572d2025-02-05T18:17:54ZengThe University of ToledoTranslation2469-67062025-02-0113S110.46570/utjms-2025-1536Borrelia burgdorferi Tolerance-Associated Changes in Gene Expression of Murine MacrophagesSophia ChenRicki DengAnis HadiSara NandwanaWilliam Ryan0John B. PresloidR. Mark WootenUT COMLS Lyme disease, caused by the spirochete Borrelia burgdorferi (Bb), is the most prevalent vector-borne disease in the United States. Macrophages, key cellular players in the innate immune response, exhibit diminished functionality over time during Bb infection, potentially leading to chronic infection. This study explores the transcriptional changes associated with macrophages that develop tolerance to Bb. Using RNA sequencing of murine bone marrow-derived macrophages exposed to Bb, we identified differentially expressed genes and dysregulated pathways between productively stimulated and tolerized macrophages. Key findings revealed significant downregulation of type-I interferon signaling and associated immune responses, suggesting mechanisms of immune tolerance. Additionally, connectivity analysis identified potential drug candidates for repurposing to enhance macrophage activity. Our results underscore the complexity of macrophage responses to Bb and provide a foundation for future research to develop targeted therapies aimed at modulating immune responses and improving treatment outcomes for Lyme disease patients. Ultimately, these findings offer new insights into the pathogenesis and potential treatment strategies for Lyme disease. https://openjournals.utoledo.edu/index.php/translation/article/view/1536Borrelia burgdorferiLyme diseasemacrophagetolerancetranscriptional profilingdrug discovery |
| spellingShingle | Sophia Chen Ricki Deng Anis Hadi Sara Nandwana William Ryan John B. Presloid R. Mark Wooten Borrelia burgdorferi Tolerance-Associated Changes in Gene Expression of Murine Macrophages Translation Borrelia burgdorferi Lyme disease macrophage tolerance transcriptional profiling drug discovery |
| title | Borrelia burgdorferi Tolerance-Associated Changes in Gene Expression of Murine Macrophages |
| title_full | Borrelia burgdorferi Tolerance-Associated Changes in Gene Expression of Murine Macrophages |
| title_fullStr | Borrelia burgdorferi Tolerance-Associated Changes in Gene Expression of Murine Macrophages |
| title_full_unstemmed | Borrelia burgdorferi Tolerance-Associated Changes in Gene Expression of Murine Macrophages |
| title_short | Borrelia burgdorferi Tolerance-Associated Changes in Gene Expression of Murine Macrophages |
| title_sort | borrelia burgdorferi tolerance associated changes in gene expression of murine macrophages |
| topic | Borrelia burgdorferi Lyme disease macrophage tolerance transcriptional profiling drug discovery |
| url | https://openjournals.utoledo.edu/index.php/translation/article/view/1536 |
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